Model bx51

Manufactured by Olympus

Sourced in Japan

The Olympus BX51 is an upright microscope designed for a wide range of applications in research and analysis. It features a sturdy and ergonomic design, providing a stable platform for precise observations. The BX51 is equipped with advanced optics and illumination systems to deliver high-quality images.

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Lab products found in correlation

Saponin, by Merck Group (2 mentions) Paraformaldehyde (pfa), by Merck Group (2 mentions) Hematoxylin, by Solarbio (2 mentions) Absolute ethanol, by Merck Group (1 mentions) Dab substrate kit, by Zhongshan Golden Bridge Biotechnology (1 mentions) Vinblastine, by Merck Group (1 mentions) Antifade mounting medium, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Model szx12, by Olympus (1 mentions) Szx12, by Olympus (1 mentions) Alexa flour 488, by Thermo Fisher Scientific (1 mentions) Anti ha antibody, by Cell Signaling Technology (1 mentions) Dab plus chromogen, by Agilent Technologies (1 mentions) Model fv1000, by Olympus (1 mentions) Dapi 4 6 diamidino 2 phenylindole, by Thermo Fisher Scientific (1 mentions) Anti laminin antibody, by Merck Group (1 mentions) Cm1850, by Leica (1 mentions) Mouse anti nestin, by Merck Group (1 mentions) Alexa fluor 488 goat anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions) Rabbit anti proteasome 20s α β, by Abcam (1 mentions)

Spelling variants of this product

System microscope Model BX51 (6 citations) Model BX51 microscope (2 citations)

33 protocols using model bx51

Immunohistochemical Analysis of Frog Gonad Development

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The primary antibodies against Vasa [20] and CYP17 were prepared in our laboratory and the anti-laminin antibody was purchased from Sigma-Aldrich.
Gonads were surgically excised from Wt and Tg frogs just after metamorphosis, fixed and frozen as previously described [21] . Frozen tissues were cut at 8-µm thickness with a Leica cryostat (Leica, CM1850) and placed on glass slides. The sections were incubated overnight at 4°C with antibodies at 1∶1,000 dilution. Following incubation with Alexa Fluor 488 goat anti-rabbit or anti-mouse secondary antibodies (Life Technologies), slides were counterstained with DAPI (4′, 6-diamidino-2-phenylindole; Life Technologies). Fluorescent signals were detected under fluorescence (OLYMPUS, model BX51) and confocal (OLYMPUS, model FV1000) microscopy. Sections were also counter-stained with Hematoxylin & Eosin and subjected to histological observations under a light microscope (OLYMPUS, model BX51).

Fujii J., Kodama M., Oike A., Matsuo Y., Min M.S., Hasebe T., Ishizuya-Oka A., Kawakami K, & Nakamura M. (2014). Involvement of Androgen Receptor in Sex Determination in an Amphibian Species. PLoS ONE, 9(5), e93655.

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Phage Control of Catheter Biofilms

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Epifluorescence observations: To assess the ability of phages to control the biofilms formed on silicone catheters, catheter sections were stained with DAPI. Briefly, 0.5 cm sections of phage-coated or non-coated catheters taken at each time points were stained with 100 μl (100 μg.mL^-1) of DAPI and placed in the dark for 10 min. The excess of dye was removed using absorbent paper, the catheter sections were placed on microscope slides and analyzed using an epifluorescence microscope (Olympus, Model BX51, Hamburg, Germany) equipped with a CCD camera (Olympus, Model DP72) with a filter-block for DAPI fluorescence (Ex 365–370; Barrier 400; Em LP 421).
Scanning electron microscopy (SEM) observations: SEM observations were performed on washed (PBS) catheter sections, which had been gradually dehydrated in absolute ethanol (Merck) solutions (15 min each in 10, 25, 40, 50, 70, 80, and 100% v/v). The catheter sections, kept in a desiccator until observed, were sputter coated with gold and observed with an S-360 scanning electron microscope (Leo, Cambridge, USA).

Melo L.D., Veiga P., Cerca N., Kropinski A.M., Almeida C., Azeredo J, & Sillankorva S. (2016). Development of a Phage Cocktail to Control Proteus mirabilis Catheter-associated Urinary Tract Infections. Frontiers in Microbiology, 7, 1024.

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Histopathological Analysis of Hind Paw Bones

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The bones of the hind paws were excised on day 29 for histological analysis. Excised bones of the ipsilateral limbs were ﬁxed in sterile Bouin's ﬂuid (1% picric acid, 9.5% formaldehyde, 5% acetic acid), decalciﬁed, sectioned, and stained with hematoxylin and eosin. Stained tissues were examined under a microscope (Model BX51, Olympus America Inc., Center Valley, PA) for histopathological changes in joints.

Annan P., Osafo N., Ossei P.P, & Abotsi W.K. (2022). Anti-Arthritic Effect of the Hydroethanolic Root Extract of Psydrax subcordata in Rats. Advances in Pharmacological and Pharmaceutical Sciences, 2022, 9748382.

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Immunohistochemical Analysis of Liver Cancer Biomarkers

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The liver cancer tissue array slides with normal tissue controls (9 AFP-negative HCC tissues/10 normal tissues, including pathological diagnosis and clinical information) were purchased (Outdo Biotech Co Ltd, Shanghai, China) and used to detect the expression of the three antigen proteins. Tissue array slides were baked for 1 h and deparaffinized with xylene, and dehydrated with ethanol. Antigen retrieval was performed by microwave heating method in citrate antigen retrieval solution for 20 min. After incubation with acid methanol for 15 min, goat serum blocking solution was used to prevent nonspecific binding of antibodies. The tissue microarrays were incubated with polyclonal NPM1 antibody, polyclonal 14-3-3zeta antibody or polyclonal MDM2 antibody (1:100 dilution) for overnight at 4 °C. The HRP Detection System (HRP streptavidin label and polyvalent biotinylated link) and DAB Substrate Kit (Zhongshan Golden Bridge Biotechnology Co Ltd) were used as detecting reagents. The sections were counterstained with hematoxylin, dehydrated, and mounted. The slides were observed by light microscopy (Model BX51; Olympus, Tokyo, Japan).

Wang T., Liu M., Zheng S.J., Bian D.D., Zhang J.Y., Yao J., Zheng Q.F., Shi A.M., Li W.H., Li L., Chen Y., Wang J.H., Duan Z.P, & Dong L. (2017). Tumor-associated autoantibodies are useful biomarkers in immunodiagnosis of α-fetoprotein-negative hepatocellular carcinoma. World Journal of Gastroenterology, 23(19), 3496-3504.

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Shrimp Histological Proliferation Assay

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There was one test solution (EM-L) and one exposure time (24 h). Shrimp were injected individually with 20 µl EM-L and paced in seawater. Shrimp injected with 20 µl of marine saline served as controls. After 24 h, shrimp were then sampled and injected with vinblastine (V1377, Sigma) to inhibit mitosis following previously described methods [25] (link). Shrimp were dissected and HPTs sampled and fixed with Davidson's fixative solution following the procedures previously described [13] (link). Details of HPTs sampling, paraffin block preparation, sectioning, staining with hematoxylin and eosin or propidium iodide, and preparation of permanent slides followed procedures previously described [25] (link), [27] (link). HPTs were observed with optical microscopy (Model BX51, Olympus, Tokyo, Japan). Optical micrographs and fluorescent photographs were both taken. The numbers of proliferating and mitotic cells were counted to establish the percentage of proliferation cells and the mitotic index.

Chen Y.Y., Chen J.C., Lin Y.C., Kitikiew S., Li H.F., Bai J.C., Tseng K.C., Lin B.W., Liu P.C., Shi Y.Z., Kuo Y.H, & Chang Y.H. (2014). Endogenous Molecules Induced by a Pathogen-Associated Molecular Pattern (PAMP) Elicit Innate Immunity in Shrimp. PLoS ONE, 9(12), e115232.

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Quantifying Mitochondrial Distribution in Hippocampus

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After following the above protocol, sections with dorsal hippocampal region were collected and separated in 12-well plate, washed with 0.1 M PBS, permeabilized with 0.25% Triton X-100, and blocked with 1.5% goat serum for 3 h at RT and then washed with 0.1 M PBS (pH 7.4) and incubated with mouse anti-COX IV (1 : 200) for 24 h at 4°C. The sections were then washed and incubated with Alexafluor-488 conjugated goat anti-mouse IgG (1 : 100) for 3 h at RT. Subsequently sections were treated with DAPI (Sigma) for 2-3 min. Finally, the sections were thoroughly rinsed thrice in distilled water, mounted onto gelatin-coated slides, and mounted in antifade mounting medium (Invitrogen). Fluorescence images were acquired on fluorescence microscope (Olympus, Model BX 51) and the mitochondrial distribution in CA1 and CA3 region was identified through intensity variation by Image J software in six random fields of 0.1 mm².

Jain K., Prasad D., Singh S.B, & Kohli E. (2015). Hypobaric Hypoxia Imbalances Mitochondrial Dynamics in Rat Brain Hippocampus. Neurology Research International, 2015, 742059.

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Caspase 3 Immunohistochemistry in Tissue

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In brief, sections were washed in 0.1 M Phosphate Buffer Saline (pH 7.4) for 30 min, and treated with 0.3% hydrogen peroxide for 30 min to inhibit endogenous peroxidase activity. Sections were washed in 0.1 M PBS, permeabilized with 0.25% Triton X-100 and blocked with 1.5% goat serum for 3 h at room temperature. They were then incubated with rabbit anti-Caspase 3 (1∶200) antibodies for 48 hours at 4°C. The sections were then washed and incubated with biotinylated goat anti-rabbit immunoglobulin (1∶100) for 3 h at room temperature, washed with 0.1 M PBS (pH 7.4) and incubated with avidin-biotin complex tagged with horse redox peroxidase enzyme prior to development with 3, 3′-diaminobenzidine (ABC kit, SantaCruz). Digital images were acquired using a light microscope (Olympus, Model BX 51, Japan) and the immunoreactive neurons for Caspase 3 were counted by Image Pro-Plus 5.1 software in six random fields of 0.1 mm².

Baitharu I., Jain V., Deep S.N., Shroff S., Sahu J.K., Naik P.K, & Ilavazhagan G. (2014). Withanolide A Prevents Neurodegeneration by Modulating Hippocampal Glutathione Biosynthesis during Hypoxia. PLoS ONE, 9(10), e105311.

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Swarming Crustacean Collection from Korean Islands

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The specimens were collected using a light trap (Holmes and O’Connor 1988 (link); Kim 1992 ) from the Dokdo and Ulleung Islands (East Sea), Korea. The collected specimens were fixed in 80% ethanol, moved to the laboratory, and stored in 95% ethanol. The specimens were identified with a stereomicroscope (Model SZX12; Olympus, Japan). Photographs of the whole body were taken with a microscope equipped with a digital camera (eXcope T500; DIXI Science, Korea) and complemented by Helicon Focus v. 7.7.5 (Helicon Soft Ltd., Kharkiv, Ukraine). The body length was measured from the anterior tip of the carapace to the posterior end of pleonite 6. The lengths of the appendages were measured along the mid-line of each appendage. The whole body was drawn using a stereomicroscope (Olympus SZX12) with a drawing tube. Later, the samples were transferred to glycerin for dissection under a stereomicroscope (Olympus SZX12). Drawing of the appendages was performed using a light microscope (Model BX51; Olympus, Japan) with a drawing tube. Type specimens were deposited at the National Institute of Biological Resources (NIBR), Incheon, Korea.

Kim S.H, & Lee T. (2024). Dimorphostylispilocorpus sp. nov. (Crustacea, Cumacea, Diastylidae), a new cumacean from Korean waters. ZooKeys, 1193, 1-18.

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Poxvirus Immunofluorescence Microscopy

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RAW cells were seeded onto 13 mm glass coverslips and infected with each poxvirus at a MOI = 10. At the respective time point of infection, the cells were fixed with 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) and permeabilized in 0.1% saponin (Sigma-Aldrich, St. Louis, MO, USA). The cells were labelled with 1:100 dilution of Anti-VACV and next, anti-mouse IgG conjugated to FITC. The stained cells were mounted onto slides using Dakocytomation (Dako, Palo Alto, CA, USA) and visualized using florescence microscope (Model BX51, Olympus, Tokyo, Japan).

Wong P.S., Sutejo R., Chen H., Ng S.H., Sugrue R.J, & Tan B.H. (2018). A System Based-Approach to Examine Cytokine Response in Poxvirus-Infected Macrophages. Viruses, 10(12), 692.

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Immunohistochemical Analysis of CAIA Mice

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Knee joints from the right hind limb, liver, spleen and kidney from WT mice with and without CAIA at day 10 were fixed in 4% paraformaldehyde and examined by IHS for GFP. Anti-GFP polyclonal rabbit (dilution 1:200) and the secondary antibody (goat anti-rabbit, Alexa Flour 488 (1:200 dilution) (Invitrogen)) were used to detect GFP. Sections were visualized under UV-light using an Olympus (Model - BX51) microscope. The presence of green fluorescence under UV-light indicated the presence of GFP expression in tissues.
Additionally, we examined for the presence of HA in the synovium from the knee joints of mice injected i.p. with AdhMAp44 and AdmMAp44 at day 10. At the time of sacrifice liver, spleen, kidney and knee joints were collected and fixed in 10% neutral-buffered formalin, processed and sections were cut. After staining with anti-HA antibody (dilution 1:1000) (Cell Signal), and the development of color using anti-Rabbit En Vision plus Polymer HRP-conjugated followed by DAB plus Chromogen (Dako), the sections were visualized by light microscopy and photographed.

Banda N.K., Mehta G., Kjaer T.R., Takahashi M., Schaack J., Morrison T.E., Thiel S., Arend W.P, & Holers V.M. (2014). Essential Role for the Lectin Pathway in Collagen Antibody-Induced Arthritis Revealed Through Use of Adenovirus Programming Complement Inhibitor MAp44 Expression. Journal of immunology (Baltimore, Md. : 1950), 193(5), 2455-2468.

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