Hypersil gold aq, by Thermo Fisher Scientific - Product details

1

HPLC-HRMS Analysis of Compounds

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HPLC analysis was
performed on a Hypersil GOLD aQ (ThermoFisher Scientific) column (100
mm × 2.1 mm, 1.9 μm) using a solvent system consisting
of A: H₂O with 0.02% AcOH and B: MeCN with 0.02% AcOH (linear
gradient from 0 to 100% B in 30 min) at a flow rate of 0.2 mL/min
at 30 °C and UV detection at 254 nm. Fractions detected using
low-resolution mass spectra (HRMS) were obtained on a LC QTOF mass
spectrometer. Conditions B: LC-HRMS analyses were performed
on a Q Exactive mass spectrometer (Thermo Fisher Scientific), equipped
with an electrospray ionization source (H-ESI II probe) coupled with
an Accela 1250 Pump (Thermo Fisher Scientific). Samples were injected
onto a Thermo Fisher Hypersil GOLD aQ chromatography column (100 mm
× 2.1 mm, 1.9 μm particle size). The flow rate was set
at 0.3 mL/min, and the mobile phase consisted of water containing
0.02% acetic acid (solvent A) and acetonitrile containing 0.02% acetic
acid (solvent B). The gradient program was as follows: 0 to 5 min,
2% B; 5 to 20 min, up to 70% B; 20 to 30 min, came back to 98% B and
2 min of equilibration. The column temperature was maintained at 30
°C, and the temperature of the autosampler was set at 4 °C.
MS analyses were performed in a full scan negative ion mode with a
scan range from 200 to 1500 m/z.

Kitoun C., Saidjalolov S., Bouquet D., Djago F., Remaury Q.B., Sargueil B., Poinot P., Etheve-Quelquejeu M, & Iannazzo L. (2023). Traceless Staudinger Ligation to Access Stable Aminoacyl- or Peptidyl-Dinucleotide. ACS Omega, 8(4), 3850-3860.

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2

Automated Vitamin D Extraction and Separation

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Example 2

Sample injection was performed with a Cohesive Technologies Aria TX-4 TFLC system using Aria OS V 1.5.1 or newer software.

The TFLC system automatically injected an aliquot of the above prepared samples into a Cohesive Technologies C8XL online extraction column (50 μm particle size, 005×50 mm, from Cohesive Technologies, Inc.) packed with large particles. The samples were loaded at a high flow rate to create turbulence inside the extraction column. This turbulence ensured optimized binding of derivatized vitamin D to the large particles in the column and the passage of excess derivatizing reagent and debris to waste.

Following loading, the sample was eluted off to the analytical column, a Thermo Hypersil Gold Aq analytical column (5 μm particle size, 50×2.1 mm), with a water/ethanol elution gradient. The HPLC gradient was applied to the analytical column, to separate vitamin D from other analytes contained in the sample. Mobile phase A was water and mobile phase B was ethanol. The HPLC gradient started with a 35% organic gradient which was ramped to 99% in approximately 65 seconds.

US11280799B2. Mass spectrometric determination of non-derivatized, non-metabolized vitamin D (2022-03-22). QUEST DIAGNOSTICS INVESTMENTS INCORPORATED [US]. Inventors: Brett Holmquist [US], Nigel Clarke [US].

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3

Automated Vitamin D Extraction and Analysis

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Example 2

Sample injection was performed with a Cohesive Technologies Aria TX-4 TFLC system using Aria OS V 1.5.1 or newer software.

The TFLC system automatically injected an aliquot of the above prepared samples into a Cohesive Technologies C8XL online extraction column (50 μm particle size, 005×50 mm, from Cohesive Technologies, Inc.) packed with large particles. The samples were loaded at a high flow rate to create turbulence inside the extraction column. This turbulence ensured optimized binding of derivatized vitamin D to the large particles in the column and the passage of excess derivatizing reagent and debris to waste.

Following loading, the sample was eluted off to the analytical column, a Thermo Hypersil Gold Aq analytical column (5 μm particle size, 50×2.1 mm), with a water/ethanol elution gradient. The HPLC gradient was applied to the analytical column, to separate vitamin D from other analytes contained in the sample. Mobile phase A was water and mobile phase B was ethanol. The HPLC gradient started with a 35% organic gradient which was ramped to 99% in approximately 65 seconds.

US11852636B2. Mass spectrometric determination of non-derivatized, non-metabolized vitamin D (2023-12-26). QUEST DIAGNOSTICS INVESTMENTS INCORPORATED [US]. Inventors: Brett Holmquist [US], Nigel Clarke [US].

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4

Passion Fruit Metabolome Analysis by UHPLC

Cited 1 time

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Passion fruit extracts were dissolved in methanol : water (0.2% formic acid), vortexed, and sonicated for 5 minutes. All chromatographic analysis was accomplished in a UHPLC Dionex Ultimate 3000 (Thermo Scientific, Sunnyvale, CA - USA) equipped with a binary pump (HP G3400RS) and a Hypersil GOLD Aq (ThermoScientific, Sunnyvale, CA, USA) 100 x 2.1 mm, 1.9 μm column at 30°C. Mobile phase A consisted of aqueous ammonium formate (0.2%) and B of acetonitrile with ammonium formate (0.2%). The initial gradient was set at 100% A switching linearly to 100% B over 8 min, then held at 100% B for 4 min before returning to 100% A in 1 min. Total run time was 13 min with 3 min postruns [20 (link)]. The identification was done with full-scan acquisition and ion extraction chromatogram (EIC) mode [M+H]⁺, and a precision of Δ_ppm < 0.001 using a mixed solution of external standards and comparable calibration curves (concentration range 0.05 to 5.00 μg/mL).

Carmona-Hernandez J.C., Taborda-Ocampo G, & González-Correa C.H. (2021). Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits. International Journal of Food Science, 2021, 8871301.

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5

Automated Vitamin D Metabolite Analysis

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Example 2

Sample injection was performed with a Cohesive Technologies Aria TX-4 TFLC system using Aria OS V 1.5.1 or newer software.

The TFLC system automatically injected an aliquot of the above prepared samples into a Cohesive Technologies C8XL online extraction column (50 μm particle size, 005×50 mm, from Cohesive Technologies, Inc.) packed with large particles. The samples were loaded at a high flow rate to create turbulence inside the extraction column. This turbulence ensured optimized binding of derivatized vitamin D metabolites to the large particles in the column and the passage of excess derivatizing reagent and debris to waste.

Following loading, the sample was eluted off to the analytical column, a Thermo Hypersil Gold Aq analytical column (5 μm particle size, 50×2.1 mm), with a water/ethanol elution gradient. The HPLC gradient was applied to the analytical column, to separate vitamin D metabolites from other analytes contained in the sample. Mobile phase A was water and mobile phase B was ethanol. The HPLC gradient started with a 35% organic gradient which was ramped to 99% in approximately 65 seconds.

US11761968B2. Mass spectrometry of steroidal compounds in multiplexed patient samples (2023-09-19). QUEST DIAGNOSTICS INVESTMENTS INCORPORATED [US]. Inventors: Brett Holmquist [US], Nigel Clarke [US].

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6

Automated Vitamin D Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 2

Sample injection was performed with a Cohesive Technologies Aria TX-4 TFLC system using Aria OS V 1.5.1 or newer software.

The TFLC system automatically injected an aliquot of the above prepared samples into a Cohesive Technologies C8XL online extraction column (50 μm particle size, 005×50 mm, from Cohesive Technologies, Inc.) packed with large particles. The samples were loaded at a high flow rate to create turbulence inside the extraction column. This turbulence ensured optimized binding of derivatized vitamin D to the large particles in the column and the passage of excess derivatizing reagent and debris to waste.

Following loading, the sample was eluted off to the analytical column, a Thermo Hypersil Gold Aq analytical column (5 μm particle size, 50×2.1 mm), with a water/ethanol elution gradient. The HPLC gradient was applied to the analytical column, to separate vitamin D from other analytes contained in the sample. Mobile phase A was water and mobile phase B was ethanol. The HPLC gradient started with a 35% organic gradient which was ramped to 99% in approximately 65 seconds.

US10753950B2. Mass spectrometric determination of non-derivatized, non-metabolized vitamin D (2020-08-25). QUEST DIAGNOSTICS INVESTMENTS INCORPORATED [US]. Inventors: Brett Holmquist [US], Nigel Clarke [US].

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7

Automated Vitamin D Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 2

Sample injection was performed with a Cohesive Technologies Aria TX-4 TFLC system using Aria OS V 1.5.1 or newer software.

The TFLC system automatically injected an aliquot of the above prepared samples into a Cohesive Technologies C8XL online extraction column (50 μm particle size, 005×50 mm, from Cohesive Technologies, Inc.) packed with large particles. The samples were loaded at a high flow rate to create turbulence inside the extraction column. This turbulence ensured optimized binding of derivatized vitamin D to the large particles in the column and the passage of excess derivatizing reagent and debris to waste.

Following loading, the sample was eluted off to the analytical column, a Thermo Hypersil Gold Aq analytical column (5 μm particle size, 50×2.1 mm), with a water/ethanol elution gradient. The HPLC gradient was applied to the analytical column, to separate vitamin D from other analytes contained in the sample. Mobile phase A was water and mobile phase B was ethanol. The HPLC gradient started with a 35% organic gradient which was ramped to 99% in approximately 65 seconds.

US11549954B2. Mass spectrometric determination of non-derivatized, non-metabolized vitamin D (2023-01-10). QUEST DIAGNOSTICS INVESTMENTS INCORPORATED [US]. Inventors: Brett Holmquist [US], Nigel Clarke [US].

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8

Automated Vitamin D Metabolite Separation

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Example 2

Sample injection was performed with a Cohesive Technologies Aria TX-4 TFLC system using Aria OS V 1.5.1 or newer software.

The TFLC system automatically injected an aliquot of the above prepared samples into a Cohesive Technologies C8XL online extraction column (50 μm particle size, 005×50 mm, from Cohesive Technologies, Inc.) packed with large particles. The samples were loaded at a high flow rate to create turbulence inside the extraction column. This turbulence ensured optimized binding of derivatized vitamin D metabolites to the large particles in the column and the passage of excess derivatizing reagent and debris to waste.

Following loading, the sample was eluted off to the analytical column, a Thermo Hypersil Gold Aq analytical column (5 μm particle size, 50×2.1 mm), with a water/ethanol elution gradient. The HPLC gradient was applied to the analytical column, to separate vitamin D metabolites from other analytes contained in the sample. Mobile phase A was water and mobile phase B was ethanol. The HPLC gradient started with a 35% organic gradient which was ramped to 99% in approximately 65 seconds.

US11105821B2. Vitamin D metabolite determination utilizing mass spectrometry following derivatization (2021-08-31). Quest Diagnostics Investments Incorporated [US]. Inventors: Brett Holmquist [US], Nigel J. Clarke [US], Richard E Reitz [US].

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9

Isocratic LC-MS Analysis of Small Molecules

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The analysis was performed with a Wadose LC isocratic pump, interfaced with a Q-Exactive Hybrid Quadrupole-Orbitrap mass spectrometer equipped with an ESI source (Thermo Fisher Scientific, Waltham, MA, USA). The MS functions were controlled by the Xcalibur data system (Thermo Fisher Scientific), whereas injection and HPLC solvent elution were monitored and controlled by our software developed on LabVIEW. The analytical column was a semi-polar Hypersil Gold aQ (50 × 1 mm, 1.9 µm, 175 Å, Thermo Fisher Scientific). The mobile phase consisted of acetonitrile (ACN)–0.1% formic acid and water–0.1% formic acid. Elution was performed with 10% and 20% ACN at a constant flow rate of 110 µL·min⁻¹. Experiments were conducted at 40 °C.

Ribette T., Leroux B., Eddhif B., Allavena A., David M., Sternberg R., Poinot P, & Geffroy-Rodier C. (2019). Primary Step Towards In Situ Detection of Chemical Biomarkers in the UNIVERSE via Liquid-Based Analytical System: Development of an Automated Online Trapping/Liquid Chromatography System. Molecules, 24(7), 1429.

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10

Automated SPE-LC-MS/MS for Sample Pretreatment

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To ensure an effective and reproducible sample pre-treatment procedure, online SPE optimization experiments focusing on the type of SPE sorbent were performed. As described under Section 2.3, the online SPE cartridges tested were the polymeric PLRP-s cartridge from Agilent, the Hypersil Hypercarb and Hypersil GOLD aQ cartridges from Thermo Fisher, and the Oasis HLB cartridge from Waters. The loading solution was composed of 0.1% (v/v) formic acid in water and methanol (gradient given in Table 1).
The automated SPE-LC-MS/MS procedure began with loading (Figure 1a) of 900 µL water sample onto the SPE cartridge for 1.1 min at a flow rate of 1 mL min⁻¹. While the sample matrix flowed to waste, the analytes were retained and concentrated on the SPE column. The analytical column was simultaneously equilibrated by the HPLC pump Then, by activating the divert valve of the column switching array (Figure 1b), the concentrated sample was flashed out of the SPE column and passed to the analytical column, where the analytes were separated and transferred for detection to the mass spectrometry equipment. At 10.0 min, the switching valve was returned to the load position to re-equilibrate both the SPE and the chromatographic column for 5.0 min. The detailed SPE conditions are presented in Table 1 along with the LC gradient program.

Belay M.H., Precht U., Mortensen P., Marengo E, & Robotti E. (2022). A Fully Automated Online SPE-LC-MS/MS Method for the Determination of 10 Pharmaceuticals in Wastewater Samples. Toxics, 10(3), 103.

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Hypersil gold aq

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26 protocols using hypersil gold aq

HPLC-HRMS Analysis of Compounds

Automated Vitamin D Extraction and Separation

Automated Vitamin D Extraction and Analysis

Passion Fruit Metabolome Analysis by UHPLC

Automated Vitamin D Metabolite Analysis

Automated Vitamin D Extraction and Analysis

Automated Vitamin D Extraction and Analysis

Automated Vitamin D Metabolite Separation

Isocratic LC-MS Analysis of Small Molecules

Automated SPE-LC-MS/MS for Sample Pretreatment

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