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4 protocols using 5 aminovaleric acid

1

Expressing and Analyzing GABA-ρ Receptors

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The subcloned human GABA-ρ1 cDNA into pcDNA1.1 (Invitrogen, San Diego, CA, USA) was generously donated by Dr. George Uh1 (National Institute for Drug Abuse, Baltimore, MD, USA). The subcloned human GABA-ρ2 cDNA into PKS (Invitrogen) was generously provided by Dr. Garry Cutting (Center for Medical Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA).
GABA, muscimol, β-alanine, 5-amino valeric acid, glycine, isoguvacine, and imidazole-4-acetic acid were purchased from Sigma-Aldrich (Sigma-Aldrich Pty Ltd., Castle Hill, NSW 1765, Australia). TACA and CACA were prepared as previously reported [3 (link)].
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2

Recombinant Expression of Human GABA Receptor

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Human ρ1 cDNA subcloned into pcDNA1.1 (Invitrogen, San Diego, CA, USA) was kindly provided by Dr. George Uh1 (National Institute for Drug Abuse, Baltimore, MD, USA).
(GABA, 5-aminovaleric acid, β-alanine, glycine, isoguvacine, 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol (THIP) also known as Gaboxadol, were purchased from Sigma-Aldrich (Sigm-Aldrich Pty Ltd, Castle Hill, NSW 1765 Australia). 1,2,5,6-Tetrahydropyridin-4-yl)methylphosphinic acid (TPMPA) [31 (link)], trans-aminocrotonic acid (TACA) and cis-aminocrotonic acid (CACA) [32 (link)], and 2-aminoethylmethane thiosulfonate (MTSEA) [33 (link)] were prepared as previously reported.
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3

Comprehensive Metabolite Profiling Protocol

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L-valine, 5-aminovaleric acid, N,O-bis-(trimethylsilyl) trifluoroacetamide (BSTFA), trimethylchlorosilane (TMCS), tert-butyl methyl ether (MTBE), N-(5-methyl-3-oxohexyl)alanine, glucoheptonic acid, homovanillic acid, dimethoxy benzoic acid, pipecolic acid, diaminopelargonic acid, imidazol lactate, glyceric acid, rhamnose, D-xylose, 3-hydroxyvaleric acid, sorbitol, mannitol, arabitol, short-chain fatty acids (SCFAs; acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids), ursodeoxycholic acid, chenodeoxycholic acid, deoxycholic acid, cholic acid, acetonitrile (ACN), formic acid, and methanol (MeOH) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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4

Modification of Montmorillonite Clay

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The detailed procedure for the modification of Na-MMT clay is described elsewhere57 ,58 (link). In brief, the 5-aminovaleric acid solution was added to preheated (60 °C) MMT suspension, and the mixture solution was kept for stirring. After one hour, the obtained slurry was centrifuged and washed to remove chloride ions, then dried at 70 °C, ground, and sieved to obtain a fine powder. Na-MMT clay was procured from Clay Minerals Respiratory at the University of Missouri, Columbia, and 5-aminovaleric acid was obtained from Sigma-Aldrich.
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