Anti p21

N-acetyl cysteine (NAC), oxamate and cisplatin were purchased from Sigma-Aldrich (MO, USA); for animal experiments cisplatin was obtained from Teva Pharmaceutical (NC, USA). The following antibodies were used in this study: anti-p53 DO-1(Santa Cruz Biotechnology, CA, USA), anti-phospho-P53 (Cell Signaling, MA, USA), anti-p21 (EMD Millipore, Billerica, MA, USA), p-histone γ H2AX S139 (Cell signaling, MA, USA) and $\beta$ -actin (Sigma, MO, USA).

N-acetyl cysteine (NAC) were purchased from Sigma-Aldrich (St. Louis, MO). Carboplatin (CBP) (SAGENT Pharmaceuticals, Schaumburg, IL), cisplatin (CDDP) (Teva Pharmaceuticals, Sellersville, PA) and doxorubicin (Pfizer Inc, New York, NY) were obtained from the institutional inpatient pharmacy. The following antibodies were used in this study: anti-p53 DO-1(Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-phospho- P53 (Cell Signaling, Danvers, MA, USA), anti-p21 (EMD Millipore, Billerica, MA, USA), anti-PARP (Cell Signaling, Danvers, MA, USA), ATM (Santa Cruz Biotechnology, Santa Cruz, CA, USA), p-ATM (EMD Millipore, Billerica, MA, USA), p-Histone H2AX S139 (Cell signaling, Danvers, MA, USA), LDHa, LDHb (Abcam, Cambridge, MA, USA) and β-actin (Sigma, St. Louis, MO, USA).

For immunostaining, tissues were fixed in Zn-formalin for 24 hr and embedded in paraffin. Sections were deparaffinized, rehydrated, and prepared by antigen retrieval for 6 min each, and then blocked in 5% donkey serum for 1 hr at room temperature, incubated with either Rabbit anti-BNIP3 antibody at 1:100 (A5683, Abclonal), Rabbit anti-NF-kB-p65 1:400 (8242S, Cell Signaling Technology), Rabbit anti-Phospho-c-jun 1:200 (3270S, Cell Signaling Technology), Rat anti-ki67 1:100 (14-5698-82, ebiosciences), Rabbit anti-pRB-Ser807/811 1:400 (8516, Cell Signaling Technology), anti-p21 1:1000 (ZRB1141, Sigma), Rat anti-Phospho H3 1:1000 (H9908, Sigma), or Goat anti-GFP (ab6673, Abcam) at 1:250 overnight at 4°C, washed, incubated with secondary antibody AF594-anti-rabbit (A-21207, Invitrogen), Alexa594 anti-rat (A-21209, Invitrogen), and Alexa488 anti-goat (11055, Invitrogen) antibodies at 1:200 for 1 hr at room temperature, counterstained with DAPI, washed, and mounted. Slides were visualized and imaged using an Olympus IX71 inverted multicolor fluorescent microscope and a DP71 camera using ×400 magnification. MFI was calculated by measuring average intensity over a fixed threshold for all images. NF-kB staining was imaged via Zeiss LSM880 confocal microscope using ×63 oil-immersion objective, and intensity was measured on 0.8 µm z-sections.