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Eosin y disodium salt

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Eosin Y disodium salt is a pink crystalline powder that is widely used as a fluorescent dye and staining agent in various laboratory applications. It is a water-soluble synthetic compound that belongs to the group of fluorescein dyes. The primary function of Eosin Y disodium salt is to provide a distinctive color and fluorescent properties for the visualization and identification of biological samples during microscopic analysis and other laboratory procedures.

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Lab products found in correlation

N vinylcaprolactam, by Merck Group (3 mentions) Methacrylic anhydride, by Merck Group (3 mentions) Gelatin from porcine skin, by Merck Group (2 mentions) 1 hydroxybenzotriazole hydrate, by Merck Group (2 mentions) Dopamine hydrochloride, by Merck Group (2 mentions) Collagenase type 2, by Worthington (2 mentions) Mayer s hematoxylin, by Merck Group (2 mentions) Triethanolamine, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Penicillin streptomycin pen strep, by Thermo Fisher Scientific (2 mentions) Live dead viability kit, by Thermo Fisher Scientific (2 mentions) Mayer s hemalum solution, by Merck Group (2 mentions) Adenosine 5 triphosphate disodium salt hydrate, by Merck Group (2 mentions) Gelma, by Merck Group (1 mentions) Malachite green, by Merck Group (1 mentions) Ethylene carbonate, by Merck Group (1 mentions) Vinyl ethylene carbonate, by Merck Group (1 mentions) Cresol red, by Merck Group (1 mentions) Thymol blue, by Merck Group (1 mentions) Propylene carbonate, by Merck Group (1 mentions)

15 protocols using eosin y disodium salt

1

Ginger-Loaded GelMA Hydrogel Fabrication

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The ginger(fraction)-loaded GelMA(sol) was fabricated using freeze-dried GelMA, triethanolamine (TEA; Sigma-Aldrich, St. Louis, MO, USA), N-vinylcaprolactam (VC; Sigma-Aldrich, St. Louis, MO, USA), eosin Y disodium salt (Sigma-Aldrich, St. Louis, MO, USA), and the ginger(fraction). It finally comprised 5 w/v% GelMA, 1.5 w/v% TEA, 1 w/v% VC, 0.5 mM eosin Y disodium salt, and different concentrations (0, 1, and 4 w/v%) of ginger(fraction). Next, 25 µL of the ginger(fraction)-loaded GelMA mixture was coated on a 1 cm2 area of the TNC-modified surface. It was then photo-crosslinked by curing for 100 s using an LED light-curing unit (C02, Premium Plus, Bournemouth, UK) for polymerization into the hydrogel. The ginger(fraction)-loaded GelMA hydrogel (GH) on the TNC surface was freeze-dried at −80 °C for 3 days using a lyophilizer. The properties of GH−TNC surfaces were compared with that of the non-modified Ti surface. The groups were named according to the concentration (w/v%) of ginger(fraction) in the hydrogel, as shown in Figure 1.
Kim S.Y., Choi A.J., Park J.E., Jang Y.S, & Lee M.H. (2022). Antibacterial Activity and Biocompatibility with the Concentration of Ginger Fraction in Biodegradable Gelatin Methacryloyl (GelMA) Hydrogel Coating for Medical Implants. Polymers, 14(23), 5317.
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2

Polymer Solutions for Energy Storage

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TBPH containing 40 wt.% in water was purchased from Acros and concentrated through rotary evaporation under 70 bar, 40 °C to get higher concentration (50 wt.%). Microcrystalline cellulose (MCC) with the size 20 µm used in this study was purchased from Sigma Aldrich. Propylene carbonate (3, 99.7% purity), vinyl ethylene carbonate (VEC, 99% purity), ethylene carbonate (EC, 98% purity), and butylene carbonate (BC, 98% purity) were obtained from Sigma Aldrich. Dimethyl sulfoxide was supplied from Sigma Aldrich. PEG with molecular weight in the range as follows: PEG 950–1050, PEG 35,000, PEO 200,000, PEO 400,000, PEO 1,000,000 were provided from Sigma Aldrich. Methylene blue, malachite green, cresol red, thymol blue, eosin Y disodium salt and methylene orange were obtained from Sigma Aldrich.
Nguyen M.N., Kragl U., Barke I., Lange R., Lund H., Frank M., Springer A., Aladin V., Corzilius B, & Hollmann D. (2020). Coagulation using organic carbonates opens up a sustainable route towards regenerated cellulose films. Communications Chemistry, 3, 116.
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3

Photopolymerization of PEGDMA Hydrogels

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Poly(ethylene glycol) dimethacrylate (PEGDMA, Mn ≈1000g mol-1), dithiothreitol (DTT), eosin Y disodium salt, monobasic potassium phosphate (KH2PO4, 99%) and sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O, 99%) were purchased from Sigma-Aldrich.
Zhu H., Yang X., Genin G.M., Lu T.J., Xu F, & Lin M. (2018). The Relationship between Thiol-acrylate Photopolymerization Kinetics and Hydrogel Mechanics: An Improved Model Incorporating Photobleaching and Thiol-Michael Addition. Journal of the mechanical behavior of biomedical materials, 88, 160-169.
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4

Gelatin-Based Biomaterial Engineering

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Gelatin from porcine skin, methacrylic anhydride (MA), dopamine hydrochloride, (Benzotriazol-1-yloxy)tris(dimethylamino)phosphonium hexafluorophosphate (BOP), 1-Hydroxybenzotriazole hydrate (HOBt), Eosin Y disodium salt, Triethanolamine (TEA), N-Vinylcaprolactam (VC), hematoxylin-eosin y staining (H&E) solutions, Iron(III) chloride, and 3-(Trimethoxysilyl)propyl methacrylate (TMSPMA) were purchased from Sigma-Aldrich. Collagenase type II was purchased from Worthington Biochemical Co. Dulbecco's modified Eagle medium (DMEM) was purchased from Cellgro (Manassas, VA), Fetal Bovine Serum (FBS) and Dulbecco's phosphate-buffered saline (DPBS) were obtained from HyClone (Logan, UT). Live/Dead viability kit, and penicillin/streptomycin (Pen-Strep) were purchased from Invitrogen (San Diego, CA), and fresh human whole blood was purchased from ZenBio.
Baghdasarian S., Saleh B., Baidya A., Kim H., Ghovvati M., Sani E.S., Haghniaz R., Madhu S., Kanelli M., Noshadi I, & Annabi N. (2021). Engineering a naturally derived hemostatic sealant for sealing internal organs. Materials Today Bio, 13, 100199.
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5

Fabrication and Characterization of PLGA-based Biomaterials

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PLGA (50:50 DLG 5E) was purchased from Lakeshore Biomaterials (Birmingham, AL, USA). Clindamycin HCL was a generous gift from Samjin Pharmaceutical Company. PEI (MW 1.8 kDa), poly(vinyl alcohol) (PVA), 2,2,2-tribromoethanol and tert-amyl alcohol (2-methyl-2-butanol) (Avertin anesthesia component), Mayer’s hematoxylin, eosin-Y disodium salt, Nile red, tetrazolium dye 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethylsulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). BactoTM tryptic soy broth (TSB) medium was purchased from BD Biosciences (Sparks, MD, USA). The LIVE/DEAD® BaclightTM bacterial viability kit (Molecular Probes) was purchased from Life Technologies (Eugene, OR, USA). The Roswell Park Memorial Institute (RPMI) 1640 medium, trypsin, fetal bovine serum (FBS), and penicillin-streptomycin were purchased from Hyclone, Thermo Fisher Scientific Inc. (Waltham, MA, USA). Phosphate buffered saline (PBS; 20×) was purchased from Biosesang (Seoul, Korea). All other reagents and solvents used were of the highest analytical grade.
Hasan N., Cao J., Lee J., Hlaing S.P., Oshi M.A., Naeem M., Ki M.H., Lee B.L., Jung Y, & Yoo J.W. (2019). Bacteria-Targeted Clindamycin Loaded Polymeric Nanoparticles: Effect of Surface Charge on Nanoparticle Adhesion to MRSA, Antibacterial Activity, and Wound Healing. Pharmaceutics, 11(5), 236.
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6

Antimicrobial Hydrogel Wound Dressing

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Pluronic F127 (PL), alginate (AL), sodium nitrite, Mayer’s hematoxylin, eosin-Y disodium salt, 2,2,2-tribromoethanol, tert-amyl alcohol (2-methyl-2-butanol) (Avertin anesthesia component), and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glutathione (GSH) was purchased from Wako Pure Chemical (Osaka, Japan). Methicillin-resistant Staphylococcus aureus 3089 (MRSA) and multidrug-resistant Pseudomonas aeruginosa 2200 (MRPA) were purchased from the Korea National Research Resource Bank (KNRRB, Seoul, Korea). BactoTM tryptic soy broth (TSB) and DifcoTM Luria Bertani (LB) media were purchased from BD Biosciences (Sparks, MD, USA). Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), and penicillin–streptomycin were purchased from Hyclone (Thermo Fisher Scientifc Inc., Waltham, MA, USA). Masson’s trichrome stain (connective tissue stain) was purchased from Abcam (Cambridge, MA, USA). All other reagents and solvents were of analytical grade.
Cao J., Su M., Hasan N., Lee J., Kwak D., Kim D.Y., Kim K., Lee E.H., Jung J.H, & Yoo J.W. (2020). Nitric Oxide-Releasing Thermoresponsive Pluronic F127/Alginate Hydrogel for Enhanced Antibacterial Activity and Accelerated Healing of Infected Wounds. Pharmaceutics, 12(10), 926.
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7

Visualization of Sporulated Yeast Cells

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Bright field and fluorescence microscopy were performed using a Leica DM4000B microscope equipped with a Leica DFC450 C digital CCD camera. Calcofluor White and Eosin Y staining was performed on cells that were sporulated in liquid SPM for 24 h as previously described (Lin et al. 2013 (link)). Cells were first harvested and washed in 1 ml McIlvaine’s buffer (0.2 M Na2HPO4/0.1 M citric acid, pH = 6.0). Staining was then performed using 30 µl Eosin Y disodium salt (5 mg/ml, Sigma) in 500 µl McIlvaine’s buffer for 10 min at room temperature in the dark. Cells were washed twice in McIlvaine’s buffer and resuspended in 200 µl McIlvaine’s buffer containing 1 µl of 1 mg/ml Calcofluor White solution (Sigma). Fluorescence of Calcofluor White and Eosin Y was examined using the DAPI and FITC filters, respectively. At least 200 cells were counted for the presence of internalized Eosin Y staining.
Trainor B.M., Ciccaglione K., Czymek M, & Law M.J. (2021). Distinct requirements for the COMPASS core subunits Set1, Swd1, and Swd3 during meiosis in the budding yeast Saccharomyces cerevisiae. G3: Genes|Genomes|Genetics, 11(11), jkab283.
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8

Fabrication of PDMS-based Photovoltaic Devices

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TiO2 paste (SC-HT040, particle size: 15–20 nm) was purchased from Sharechem Co. (Hwaseong, Korea). Chloroplatinic acid hexahydrate (>37.5%, Pt base), and eosin Y disodium salt (>85%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). I2, KI, 2-propanol (IPA, 99.8%), and ethanol (95.0%) were obtained from Samchun Chemicals (Seoul, Korea). A poly(dimethylsiloxane), PDMS, elastomer kit (Sylgard 184 silicone elastomer base and curing agent) was purchased from Dow Corning Corp (Midland, TX, USA). PDMS was made by mixing the base and curing agent in a ratio of 10:1 by weight, degassing, and curing in an oven at 60 °C.
Kim J.H., Park S.Y., Lim D.H., Lim S.Y., Choi J, & Koo H.J. (2021). Eco-Friendly Dye-Sensitized Solar Cells Based on Water-Electrolytes and Chlorophyll. Materials, 14(9), 2150.
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9

Hematoxylin-Eosin Staining and Immunofluorescence

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For Hematoxylin-Eosin (H&E) staining, reagents included neutral buffered formalin solution 10%, phosphate buffered saline (PBS), Gill’s hematoxylin No. 3 solution, hydrochloric acid 37% (HCl), absolute ethanol, Eosin Y disodium salt, acetic acid 99.7%, xylene substitute, and Eukitt® quick-hardening mounting medium; all were obtained from Sigma Aldrich (Burlington, MA).
For immunofluorescence, reagents included methanol obtained from EMD Millipore (Darmstadt, Germany), paraformaldehyde (4% formaldehyde prepared in PBS) and Streptavidin alexa flour 488 conjugate obtained from Thermo Fisher Scientific (Waltham, MA), and goat serum, 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI), and flouromount aqueous mounting medium; all obtained from Sigma Aldrich (Burlington, MA). Primary antibodies included anti-CD68 (ab125121), anti-iNOS (ab15323), and anti-αSMA (ab5694). Secondary antibodies included biotinylated goat antirabbit IgG (ab6720) and goat antirabbit IgG alexa flour 488 (ab150077). All antibodies and an endogenous biotin/avidin blocking kit (ab64212) were obtained from Abcam (Cambridge, MA).
Oliver A.A., Guillory RJ I.I., Flom K.L., Morath L.M., Kolesar T.M., Mostaed E., Sikora-Jasinska M., Drelich J.W, & Goldman J. (2020). Analysis of vascular inflammation against bioresorbable Zn-Ag based alloys. ACS applied bio materials, 3(10), 6779-6789.
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10

Synthesis and Characterization of Polymeric Biomaterials

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4,4-azobis(4-cyanopentanoic acid) (ABCPA), poly(ethylene glycol) methyl ether (Mw 5000 g/mol) (mPEG), N,N’-Dicyclohexylcarbodiimide (DCC), 4-dimethylaminopyridine (DMAP), and p-toluenesulfonic acid, L-lactide, N-(2-hydroxypropyl)methacrylamide (HPMAm), Tin(II) 2-ethylhexanoate (SnOct2), and 4-methoxyphenol were purchased from Sigma-Aldrich. All solvents: tetrahydrofuran (THF), dichloromethane (DCM), dimethylformamide (DMF), acetonitrile (ACN), and acetone were provided by Sigma-Aldrich or Fisher Chemical. Nuclear magnetic resonance (NMR) solvents: chloroform-d (CDCl3), deuterated dimethyl sulfoxide (DMSO-d6) and deuterium oxide (D2O) were purchased from Cambridge Isotope Laboratories, Inc. Gelatin from porcine skin (Gel strength 300, type A), methacrylic anhydride, hyaluronic acid sodium salt from Streptococcus equi, glycidyl methacrylate (GM), Eosin Y disodium salt, triethanolamine (TEA) and N-vinylcaprolactam (VC), Triton X-100 were all purchased from Sigma-Aldrich.
Zhu X., Gibbons J., Garcia-Rivera J., Casadevall A, & Williamson P.R. (2001). Laccase of Cryptococcus neoformans Is a Cell Wall-Associated Virulence Factor. Infection and Immunity, 69(9), 5589-5596.
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