Tissues were prepared, deparaffinized, and rehydrated in the same fashion as in immunohistochemistry staining. We utilized the Colloidal Iron Stain Kit (Mucin Stain) from Abcam (ab150664) and followed company protocol. Briefly, tissues were pretreated with acetic acid solution, treated with Colloidal Iron Solution for 30 minutes, and rinsed in acetic acid solution. Tissues were then stained with Iron Stain Solution for 10 minutes to visualize mucin (blue) and rinsed in distilled water. Finally, tissues were stained for 30 seconds with Van Gieson’s solution to identify collagen (red), dehydrated with 100% alcohol, and mounted in synthetic resin. Protein staining quantification was measured by area fraction and mean fluorescence intensity of the dermis and epidermis in one 20x objective magnification field using NIS-Elements software.
Mucin stain
Manufactured by Abcam
Sourced in Australia
Mucin Stain is a laboratory reagent used to detect the presence of mucins, a class of glycoproteins, in tissue samples. It is a histological staining technique that helps visualize the distribution and localization of mucins within cells and tissues.
Automatically generated - may contain errors
3 protocols using mucin stain
1
Colloidal Iron Staining for Mucin
2
Mucin Depletion in DSS-Induced Colitis
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DSS-induced alterations in goblet cells and subsequent depletion in synthesis and secretion of mucin glycoprotein (MUC2) was analysed by Alcian blue staining (ab150662 Alcian Blue, pH 2.5 (Mucin Stain), Abcam, Australia) following the manufacturer’s instructions. Briefly, paraffin-embedded colon sections (n = 4/group) were stained with Alcian blue, staining the acidic sulphated mucin blue and the counterstained with Safranin O, staining the nuclei red following the method previously described [30 (link)]. Computer-assisted image analysis was performed with a Leica DM500 microscope (Leica Microsystems, Wet-zlar, Germany) and Leica ICC50 W camera (Leica Microsys-tems, Wetzlar, Germany). The staining intensity (IOD) was assessed using Image Pro Plus 7.0 (Media Cybernetics, Inc., Rockville, MD, USA) and used for comparison among groups [31 (link)].
3
Alcian Blue Staining of Goblet Cells
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DSS-induced alterations in goblet cells, and subsequent depletion in synthesis and secretion of mucin glycoprotein, were analysed by Alcian blue staining (ab150662 Alcian Blue, pH 2.5 (Mucin Stain), Abcam, Australia) following the manufacturer’s instructions. The staining intensity (IOD) was assessed using Image Pro Plus 7.0 (Media Cybernetics, Inc., Rockville, MD, USA) and used for comparison among groups [34 (link)].
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