Mouse tslp elisa kit

A total of 15 female BALB/c mice (6–8 weeks old; weighing 18±2 g) were purchased from Shanghai SLAC Laboratory Animal Center (Shanghai, China). The animals were housed under specific pathogen-free conditions under a 12-h light/dark cycle with a constant temperature of 20°C and humidity of 55%. No dietary restriction was applied. All procedures were performed in accordance with the guidelines of the Institutional Animal Care and Use Committee of Sun Yat-sen University (Guangzhou, China). Purified HDM whole-body extract was purchased from Greer Laboratories, Inc. (Lenoir, NC, USA) and dissolved in saline for intranasal instillation. TAK242 powder was purchased from Takeda Pharmaceutical Company (Osaka, Japan) and dissolved in dimethyl sulfoxide (DMSO) prior to use. A mouse TSLP ELISA kit was purchased from R&D Systems, Inc. (cat. no. MTLP00; Minneapolis, MN, USA), and IL-4, IL-13 and interferon (IFN)-γ ELISA kits were purchased from eBioscience; Thermo Fisher Scientific, Inc. (cat. nos. BMS613, BMS6015, and BMS606, respectively; Waltham, MA, USA). The present study was approved by the Ethics Committee of the Third Affiliated Hospital of Sun Yat-Sen University.

The murine keratinocyte cell line PAM212, which was derived from BALB/c mouse skin, was kindly bestowed by Dr. Yuspa (National Institutes of Health, NCI, USA).
Main reagents: RPMI-1640 culture medium (Gibco, 11875093), FBS (Gibco, 12483020), 0.25% trypsin-EDTA (Gibco, 25200114), m-xylene (sigma, 95670), 1,2,4-trimethylbenzene (sigma, 45996), formaldehyde solution (sigma, 252549), TPA (sigma, P1585), PM2.5 (bestowed by the Department of Environmental Health, School of Public Health, Fudan University), mouse TSLP ELISA kit (R&D, MTLP00), reverse transcription kit PrimeScriptTM (Takara, RR037A), and GoTap qPCR master mix (promega, A6002).