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Orca er c4742 80 12ag camera

Manufactured by Hamamatsu Photonics

The ORCA-ER C4742-80-12AG is a high-performance digital camera designed for microscopy and imaging applications. It features a 1.3-megapixel CCD sensor with 12-bit depth, enabling high-resolution image capture. The camera offers a fast frame rate and low noise, making it suitable for a variety of scientific and industrial uses.

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2 protocols using orca er c4742 80 12ag camera

1

Microscopic Analysis of DARS2 Localization

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SMR14334/pBR322-DARS2 and SMR14334/pBR322 cells were deposited on a 1% minimal poor medium agarose pad. Microscope analysis was done using an AxioImager Z1 microscope (Carl Zeiss MicroImaging, Inc) equipped with a 100× /1,4 DIC objective and a Hamamatsu ORCA-ER C4742-80-12AG camera. Fluorescence images were acquired using a GFP filter cube (41017 Endow GFP Bandpass Emission) and using an exposure time of 500 ms. The microscope pictures were processed and analyzed with Volocity (PerkinElmer), ImageJ and Adobe Illustrator software. Levels of intensity were set in ImageJ and propagated to all pictures.
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2

PNA-peptide Microscopy Analysis of Bacterial Cells

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Strain ALO4223 was grown exponentially in AB medium, diluted to OD450=0.05 and treated with PNA-peptide (8 μM) for 2 h. Fluorescence microscopy analysis was performed using an AxioImager Z1 microscope (Carl Zeiss MicroImaging, Inc), with a × 100 objective and a Hamamatsu ORCA-ER C4742-80-12AG camera, as described previously.20 (link) The images were analysed, processed and intensity adjusted using Image J Software.23 (link) Note that foci co-localization and resolution limitations may lead to underestimation of especially the number of oriC copies.
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