The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 h. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and FCS Express software (De Novo, Glendale, CA, USA).
Facscanto 2 low cytometer
Manufactured by BD
Sourced in United States
The FACSCanto II is a low-cytometry instrument designed for multicolor flow cytometry analysis. It is capable of detecting up to 8 fluorescent parameters simultaneously. The core function of the FACSCanto II is to provide researchers with a reliable and efficient tool for analyzing complex cellular samples.
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Lab products found in correlation
2 protocols using facscanto 2 low cytometer
1
Apoptosis Assay of Colon Cancer Cells
2
Annexin V Apoptosis Assay for Oral Cancer
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The apoptosis of oral cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions. Briefly, HSC-3, SAS, and OECM-1 cells (1 × 105) were treated with 0, 10 and 20 mg/mL concentrations of AGA for 72 h. Thereafter, cells were harvested and stained with PE Annexin V/7-AAD for 15 min. The stained cells were analyzed using FACSCanto II low cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and FCS Express software (De Novo, Glendale, CA, USA).
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