Hematoxylin and Eosin (H–E), Alizarin Red, RIPA buffer, and dexamethasone were purchased from FUJIFILM Wako Chemicals (Tokyo, Japan). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Nacalai Tesque Inc. (Kyoto, Japan). NucleoSpin® RNA was purchased from Macherey-Nagel (Düren, Germany). iScript cDNA-Supermix, SsoFast EvaGreen-Supermix, and TGX precast gel were obtained from Bio-Rad Laboratories (Hercules, CA, USA). Ascorbic acid and DAPI were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Polyvinylidenedifluoride (PVDF) Blocking Reagent® and Can Get Signal® Solution-1 and -2 were obtained from Toyobo Co. Ltd. (Tokyo, Japan). ECLTM Prime Western Blotting Detection Reagent was purchased from Cytiva (Tokyo, Japan).
Can get signal solution 1 and 2
Manufactured by Toyobo
Sourced in Japan
Can Get Signal® Solution-1 and -2 are laboratory products designed for signal detection and amplification. Solution-1 and Solution-2 work together to enhance signal intensity in various analytical techniques. The exact function and intended use of these products are not provided in a detailed, unbiased, and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Dexamethasone (dex), by Fujifilm (1 mentions)
Alizarin red, by Fujifilm (1 mentions)
Dulbecco s modified eagle s medium, by Nacalai Tesque (1 mentions)
Iscript cdna supermix, by Bio-Rad (1 mentions)
Polyvinylidenedifluoride pvdf blocking reagent, by Toyobo (1 mentions)
Tgx precast gel, by Bio-Rad (1 mentions)
Ripa buffer, by Fujifilm (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Nucleospin rna, by Macherey-Nagel (1 mentions)
Ssofast evagreen supermix, by Bio-Rad (1 mentions)
Ecl chemiluminescence, by GE Healthcare (1 mentions)
Blocking reagent for can get signal, by Toyobo (1 mentions)
Nitrocellulose membrane, by Bio-Rad (1 mentions)
2 protocols using can get signal solution 1 and 2
1
Molecular and Cellular Analysis of Bone Cell Differentiation
2
Amyloid-beta Oligomer Inhibition Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Aβ42 solution (10 μM) was incubated with or without each compound (DDC and 1–3, 10 or 50 μM) at 37 °C for 1 h. 1 μL (45 ng) of each Aβ sample was applied to the nitrocellulose membrane (0.2 μm pore size, Biorad), and the membrane was blocked with Blocking Reagent for Can Get Signal (TOYOBO, Tokyo, Japan). The membrane was then probed with A11 antibody39 (link) at 1 μg mL−1 (Invitrogen, Carlsbad, CA), followed by treatment with the secondary antibody (anti-rabbit IgG or anti-mouse IgG). Development was performed with ECL chemiluminescence (GE Healthcare). The first and second antibodies were diluted with Can Get Signal Solution 1 and 2 (TOYOBO), respectively. Phosphatase buffered saline plus potassium (PBS-K; 1.5 mM KH2PO4, 8.1 mM Na2HPO4, 140 mM NaCl, 2.7 mM KCl, pH 7.4) including 0.5% Tween-20 was used as a washing buffer.
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