Hnf1α

Manufactured by Santa Cruz Biotechnology

Sourced in United States

HNF1α is a transcription factor that regulates the expression of various genes involved in liver development and function. It plays a critical role in the control of metabolic pathways and is essential for the proper functioning of hepatocytes.

Automatically generated - may contain errors

Lab products found in correlation

5 protocols using hnf1α

Investigating Neuroinflammatory Mechanisms

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals, including anti-CT19 amyloid antibody and 10% neutral buffered formalin solution used in this study, were from Sigma Chemical (St. Louis, MO, USA). Specific antibodies to MAPKs, phospho-MAPKs, total tau, p-tau-T181, p-tau-S396, β-amyloid, TNF-α, MCP-1, GFAP, cleaved caspase-3, or IL-6 were purchased from Cell Signaling (Danver, MA, USA). Specific antibody to GSK-3β, p-GSK-3β-S9, CDK5, p-tau-T231, iNOS, 3-NT, CYP2E1, or NeuN was from Abcam (Cambridge, MA, USA). Antibody against p-CDK5-Tyr15, cleaved caspase-3, Bax, GAPDH, NeuN, GFAP, HSP90, HNF-1α, BNIP3, and secondary antibodies conjugated with horse radish peroxidase were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Recombinant HIV-1 Tat and gp120 (B.MN D11 strain) proteins were provided by the NIAID AIDS Reagent Program (National Institutes of Health, Bethesda, MD, USA). Neuro-2a cells were purchased from the ATCC (American Type Culture Cells (Manassas, VA). Other materials not described here were the highest grades available and/or the same, as recently described [21 (link), 22 (link)].

Cho Y.E., Lee M.H, & Song B.J. (2017). Neuronal Cell Death and Degeneration through Increased Nitroxidative Stress and Tau Phosphorylation in HIV-1 Transgenic Rats. PLoS ONE, 12(1), e0169945.

+ Open protocol

+ Expand

Protein Detection Antibodies in Hepatocyte Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Various proteins were detected by the following antibodies: ISG20 (Abcam, Cambridge, UK, 1:2000), HBV core protein (B0586, Dako, Glostrup, Denmark, 1:2000), HBsAg (ab9193, Abcam, or Dako, 1:2000), hepatocyte nuclear factor 1α (HNF1α) (sc‐393668, 1:1000, Santa Cruz Biotechnology, Dallas, TX, USA), FLAG‐M2 (A5316, 1:2000, Sigma), CEBPα (sc‐365318, 1:1000, Santa Cruz Biotechnology), HNF4α (sc‐374229, 1:2000, Santa Cruz Biotechnology), PGC‐1α (sc‐518025, 1:2000, Santa Cruz Biotechnology), and actin (A5316, 1:5000, Sigma).

Park Y.K., Lee S.Y., Lee A.R., Kim K., Kim K., Kim K, & Choi B. (2020). Antiviral activity of interferon‐stimulated gene 20, as a putative repressor binding to hepatitis B virus enhancer II and core promoter. Journal of Gastroenterology and Hepatology, 35(8), 1426-1436.

+ Open protocol

+ Expand

Protein Expression Analysis Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total membranes, protein and nuclear extracts were prepared and protein concentrations were determined as described previously [6 (link), 7 (link)]. The dilution of primary antibodies were as follows: CYP7B1 (1:2000), CYP8B1 (1:2000), CYP27A1 (1:2000), CYP3A4 (1:4000), UGT2B (1:4000), SULT2A1 (1:2000), GSTA1 (1:1000), GSTM2 (1:1000), OSTα (1:4000), OCT1 (1:2000), ABCG5 (1:3000), ABCG8 (1:2000), VDR (1:1000), RARα (1:1600), HNF1α (1:2000), HNF4α (1:2000), and LXR (1:1000) (Santa Cruz Biotechnology, Santa Cruz, CA), CYP2B6 (1:2000) (OriGene, Rockville, MD), GSTA2 (1:4000) (GeneTex, Irvine, CA), GSTA3 (1:1000), GSTA4 (1:1000), GSTM1 (1:1000), GSTM3 (1:1000), GSM4 (1:1000), PPARα (1:1000), and AHR (1:1000) (Proteintech Group, Chicago, IL, USA), OSTβ (1:500) (Sigma-Aldrich Chemical Co., St Louis, MO), ABCG2 (1:2000), FXR (1:10,000), SHP (1:1000), HNF3β (1:10,000), and NRF2 (1:10,000) (Abcam, Cambridge, MA). GAPDH (1:40,000) (Abcam) and SH-PTP1 (1:1600) (Santa Cruz) were used for normalizing data.

Chai J., Feng X., Zhang L., Chen S., Cheng Y., He X., Yang Y., He Y., Wang H., Wang R, & Chen W. (2015). Hepatic Expression of Detoxification Enzymes Is Decreased in Human Obstructive Cholestasis Due to Gallstone Biliary Obstruction. PLoS ONE, 10(3), e0120055.

+ Open protocol

+ Expand

Western Blot Analysis of Nutrient Transporters

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For western blot analysis, cultured Caco-2 cells, mouse jejunal tissues, and organoids were homogenized in RIPA buffer (Sigma) in the presence of a complete protease inhibitor cocktail (Roche, Nutley, NJ). Sixty micrograms of total protein (60μg) was loaded into an individual lane of a 4–12% mini gel (Invitrogen) and transferred to PVDF membrane. After transfer, the membrane was probed with primary antibodies [hSVCT-1 (1:1000 dilution; Abgent, CA: Cat # AP12718a), mSVCT-1 (Cat # Sc 9921), hSVCT-2 (Cat # Sc 30114), mSVCT-2 (Cat # Sc 9926), HNF1α (Cat # Sc 6547) and Sp1 (Cat # Sc 14027) 1:200 dilutions; Santa Cruz biotechnology Inc.]. The specificity of the antibodies has been previously determined from protein samples isolated from various in vitro and in vivo preparations [16 (link), 51 (link), 58 (link)–60 (link)]. Anti-β-actin mouse monoclonal antibody (in 1:3000 dilution; Santa Cruz Biotechnology, Cat # Sc 47778) was used as an internal control. The corresponding secondary antibodies (LI-COR Biosciences) in 1:30,000 dilutions were used as described previously [16 (link), 51 (link)]. Relative protein expression was quantified by normalizing the signal intensity against β-actin using application software in the Odyssey Infrared imaging suite (version 3, LI-COR Biosciences).

Subramanian V.S., Sabui S., Moradi H., Marchant J.S, & Said H.M. (2017). Inhibition of intestinal ascorbic acid uptake by lipopolysaccharide is mediated via transcriptional mechanism(s). Biochimica et biophysica acta, 1860(2), 556-565.

+ Open protocol

+ Expand

Western Blot Analysis of Liver Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-cell lysates were obtained with a boiling lysis buffer (1% SDS, 1mM Tris HCL, and 8mM Sodium orthovanadate). Liver samples were washed with PBS 1X and then grinded in lysis buffer (10 mM HEPES, 0.2 mM EDTA, 10 mM KCL, 1.5 mM MgCl₂, 150 mM NaCl, 0.01 mM DTT, 0.2% NP40 and 40U RNase out). Lysates were centrifuged at 10,000 g for 5 min at 4 °C. 40 µg of protein extracts were resolved on a 12% SDS-page gel and transferred on a nitrocellulose membrane. Membranes were blocked in 5% skim milk (2.5% for SigR1 antibody), then incubated overnight at 4 °C with specific primary antibodies: SigR1 (1/400; Covalab), ERα (1/400; H184 Santa Cruz), HNF1α (1/500; Santa Cruz). The bound antibodies were detected (Fusion, Vilbert-Lourmat, France) using corresponding horseradish peroxidase-conjugated secondary antibodies and chemiluminescence detection kit (Biorad, Marnes-la-Coquette, France). Quantification of the signals was performed by densitometry using the ImageJ software.

Villemain L., Prigent S., Abou-Lovergne A., Pelletier L., Chiral M., Pontoglio M., Foufelle F., Caruso S., Pineau R., Rebouissou S., Chevet E., Zucman-Rossi J, & Combettes L. (2020). Sigma 1 Receptor Is Overexpressed in Hepatocellular Adenoma: Involvement of ERα and HNF1α. Cancers, 12(8), 2213.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!