Sirna p38

Alpha-mangostin (99% purity), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), N-acetyl-L-cysteine (NAC), and 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazol-carbocyanine iodide (JC-1), were purchased from Sigma (St. Louis, MO, USA). Z-VAD, a pan-caspase inhibitor, was obtained from BioVision (Mountain View, CA, USA). The kinase inhibitors, PD98059 to MEK, SB203580 to p38, and SP600125 to JNK, were bought from Calbiochem (San Diego, CA). The antibodies against phosphory-ERK, phosphory-p38, phosphory-JNK, ERK, p38, JNK, phosphory-MKK3/6, MKK3/6, Bcl-2, Bax, cytochrome C, COX4, α-tubulin, β-actin, siRNA-ERK, siRNA-JNK and siRNA-p38, siRNA-ASK1, siRNA-MKK3/6 were purchased from Santa Cruz Biotechnology (Dallas, Texas). The antibodies against cleaved-caspase-3, cleaved-caspase-9, cleaved-poly-ADP-ribose polymerase (PARP), phosphory-ASK1 and ASK1 were purchased from Cell Signaling (Danvers, MA, USA). Horseradish peroxidase-labeled anti-mouse and anti-rabbit secondary antibodies were bought from Promega (Madison, WI, USA).

Timosaponin AIII (TSAIII; CFN98151) was obtained from ChemFaces (Wuhan, Hubei, PRC). The DMSO, MTT and isopropanol were obtained from Merck KGaA (Darmstadt, Germany). Primary antibodies against uPA, total-ERK, phospho-JNK, total-JNK, phospho-p38MAPK, total-p38MAPK, uPA, GAPDH and siRNA-p38 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The antibody of phospho-ERK, SOX2, Nanog, OCT4 and CD49f were purchased from Cell Signaling Technology (Danvers, MA, USA).