Hepatic triglycerides (TGs) were quantified using Triglyceride Reagent and Triglyceride Standards (Pointe Scientific). Serum alanine aminotransferase (ALT) and aspartate aminotransferase levels (AST) were quantified using ALT Reagent, AST Reagent and Catatrol I and II (Catachem). For histology, liver tissue was fixed in 10% buffered formalin, and stained with H&E or Masson’s trichrome and evaluated by a board-certified pathologist (3 (link), 24 (link)).
Triglyceride standard
Manufactured by Horiba
The Triglyceride Standards are a set of reference materials used to calibrate and verify the performance of analytical instruments that measure triglyceride levels in biological samples. These standards provide a consistent and reliable basis for ensuring the accuracy and precision of triglyceride quantification.
Automatically generated - may contain errors
Lab products found in correlation
Triglyceride reagent, by Horiba (4 mentions)
Triglycerides reagent, by Thermo Fisher Scientific (2 mentions)
D12492, by LabDiet (1 mentions)
4 hne, by Cell Biolabs (1 mentions)
Infinity triglyceride reagent, by Thermo Fisher Scientific (1 mentions)
Tissuelyser 2, by Qiagen (1 mentions)
Spectramax m series multi mode microplate reader, by Molecular Devices (1 mentions)
7 protocols using triglyceride standard
1
Quantification of Hepatic Lipids and Enzymes
2
Quantifying Hepatic Triglycerides and Liver Function
3
Hepatic Triglyceride and ALT Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hepatic triglycerides were quantified using Triglyceride Reagent and Triglyceride Standards (Pointe Scientific) as previously described13 (link),22 (link)–24 (link),26 (link). Serum alanine transaminase (ALT) levels were quantified using ALT Reagent and Catatrol I and II (Catachem). For histology, liver tissue was fixed in 10% buffered formalin, and stained with H&E13 (link),22 (link),23 ,26 (link),27 (link).
4
High-Fat Diet Induced Metabolic Dysregulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Diets: Mice were fed either a high-fat diet (HFD; Research Diets #D12492) or a chow diet (Chow; LAB Diet #5010). Glucose dysmetabolism: Fasting glucose and insulin and glucose tolerance testing were quantified after an overnight fast. Liver: Hepatic triglycerides were quantified using Triglyceride Reagent and Triglyceride Standards (Pointe Scientific); serum alanine transaminase (ALT) levels were quantified using ALT Reagent and Catatrol I and II (Catachem); lipid peroxidation was quantified using 4-hydroxynonenal (4-HNE) enzyme-linked immunosorbent assay (ELISA) reagents (Cell Biolabs)—all according to the manufacturer's instructions.
5
Serum and Fecal Triglycerides Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum TAG levels were measured using the Triglycerides Reagent (Thermo Scientific, #TR22421) and Triglyceride Standard (Pointe Scientific, #T7531STD). In brief, 2.5 μl of the standard or serum sample and 250 μl of the reagent were added to each microplate well. After incubating at 37 °C for 10 min, absorbance at 500 nm was measured using a Tecan plate reader.
For fecal TAG, feces were collected and homogenized in H2O (1/24, w/v). Chloroform/methanol (2/1, v/v) was then added to extract TAG. 300ul of the bottom chloroform layer containing TAG was transferred to a new tube and dried under nitrogen. The TAG content was measured using Triglycerides Reagent as described above.
For fecal TAG, feces were collected and homogenized in H2O (1/24, w/v). Chloroform/methanol (2/1, v/v) was then added to extract TAG. 300ul of the bottom chloroform layer containing TAG was transferred to a new tube and dried under nitrogen. The TAG content was measured using Triglycerides Reagent as described above.
6
Tissue Triglyceride Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissue TAG levels were measured using Triglycerides Reagent (Thermo Fisher Scientific, TR22421) and Triglyceride Standard (Pointe Scientific, T7531STD) according to a modified protocol (53 (link)). In brief, tissue samples were homogenized in PBS (1/30, w/v). Then, 2.5 μL of the standard or sample homogenization and 250 μL of the reagent were added to each microplate well. After incubation at 37°C for 10 minutes, absorbances at 500 nm were measured using a plate reader from Tecan.
7
Quantification of Hepatic Triglycerides
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To determine hepatic triglyceride content, 50 mg of frozen liver tissue from each animal was weighed, on ice, and then homogenized independently in 2 mL Eppendorf™ Snap-Cap Microcentrifuge Safe-Lock™ Tubes (05-402-8; Thermo Fisher Scientific) with a 5 mm stainless steel bead (69989; Qiagen) and 1 mL of isopropanol alcohol (I9526; Sigma). Samples were lysed using a TissueLyser II (85300; Qiagen) for 2 min and then incubated on ice for 1 h, vortexing two to three times during the incubation. After cold incubation, samples were centrifuged at 10,000 rpm × 15 min at 4°C, and then, the supernatant was transferred to a new tube. Triglyceride concentration was determined using the Infinity™ Triglyceride reagent (TR22421; Thermo Fisher Scientific) and Triglyceride standard (T7531-STD; Pointe Scientific) according to the manufacturer's instructions. Absorbance was determined using a SpectraMax ® M Series Multi-Mode Microplate Reader (Molecular Devices) and SoftMax Pro6 Software, V1.0 (Molecular Devices).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!