The 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2, 4, 6-Tris(2-pyridyl)-s-triazine (TPTZ), 2, 6-di-tert-butyl-4-methylphenol (BHT), vitamin C (Vc), were obtained from Solarbio Life sciences (Beijing, China). All chemical reagents including methanol, ethyl acetate (EtOAc), petroleum ether (PE), Dichloromethane (DCM), and n-butanol (n-BuOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). Folin-Ciocalteu, pyrogallol, and other analytical grade chemicals were purchased from Aladdin (Shanghai, China). Myricetin, dihydroMyricetin, quercetin, resveratrol, apigenin, baicalein, hesperitin, gallic acid, rutin, luteolin, epicatechin, epicatechin gallate, and other standards for HPLC were purchased from Yuanye Bio-Technology (Shanghai, China). Cellulase (3, 000 μ/g) and pectinase (40, 000 μ/g) were purchased from Xiya reagent (Shandong, China). α-Glucosidase (G5003) and acetylcholinesterase (AChE, C3389) were purchased from Sigma-Aldrich (St. Louis, USA). p-Nitrophenol nitrophenol α-D-glycopyranoside (pNPG), acarbose, galantamine, and cisplatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The HepG2, MCF-7, Raw264.7, and A549 cells were purchased from Conservation genetics CAS Kunming cell bank.
Resveratrol
Manufactured by Yuanye Bio-Technology
Sourced in China
Resveratrol is a natural polyphenol compound found in various plants, including grapes, berries, and peanuts. It is used in laboratory research applications as a chemical standard for analysis and assays.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Yuanye Bio-Technology (4 mentions)
Quercetin, by Yuanye Bio-Technology (4 mentions)
Luteolin, by Yuanye Bio-Technology (3 mentions)
Baicalein, by Yuanye Bio-Technology (2 mentions)
Myricetin, by Yuanye Bio-Technology (2 mentions)
Rutin, by Yuanye Bio-Technology (2 mentions)
Ellagic acid, by Yuanye Bio-Technology (2 mentions)
Chlorogenic acid, by Yuanye Bio-Technology (2 mentions)
P coumaric acid, by Yuanye Bio-Technology (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Piceatannol, by Yuanye Bio-Technology (2 mentions)
Cisplatin, by Merck Group (1 mentions)
Methanol, by Sinopharm (1 mentions)
Dihydromyricetin, by Yuanye Bio-Technology (1 mentions)
Epicatechin gallate, by Yuanye Bio-Technology (1 mentions)
Galantamine, by Merck Group (1 mentions)
Hesperitin, by Yuanye Bio-Technology (1 mentions)
Epicatechin, by Yuanye Bio-Technology (1 mentions)
N butanol n buoh, by Sinopharm (1 mentions)
Chromatographic acetonitrile, by Merck Group (1 mentions)
13 protocols using resveratrol
1
Bioactive Compounds Extraction and Evaluation
2
Neuroprotective Effects of Natural Compounds
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Nutrients including L-tyrosine(CAS No.: 60-18-4), Vitamin B1(CAS No.: 67-03-8), Vitamin B2(CAS No.: 83-88-5), Vitamin B5(CAS No.: 137-08-6), Vitamin B6(CAS No.: 58-56-0), Vitamin B12(CAS No.: 68-19-9), Quercetin(CAS No.: 117-39-5), Resveratrol(CAS No.: 501-36-0), Tea Polyphenols(CAS No.: 84650-60-2), Genistein(CAS No.: 446-72-0), Baicalein(CAS No.: 491-67-8), Luteolin(CAS No.: 491-70-3), Proanthocyanidin(CAS No.: 4852-22-6) and Naringin(CAS No.: 10236-47-2) are all purchased from Yuanye Biotechnology (Shanghai, China); CCK8 kit and ROS kit are purchased from Beyotime Biotechnology (Shanghai, China).
Instruments includes Artificial climate box (ZRQ-400); Morris water maze (JLBehav-MWMG); Shuttle box (JLBehav-STG-4); Fluorescent dye 2′, 7′-Dichlorodihydrofluorescein diacetate (DCFH-DA, Sigma, USA).
Instruments includes Artificial climate box (ZRQ-400); Morris water maze (JLBehav-MWMG); Shuttle box (JLBehav-STG-4); Fluorescent dye 2′, 7′-Dichlorodihydrofluorescein diacetate (DCFH-DA, Sigma, USA).
3
Curcumin-Resveratrol Nanoparticle Synthesis
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The Cur-RV NPs were synthesized using emulsification and evaporation method as previously described (Jin et al., 2017 (link)) with a little modification. Briefly, 20 mg of Curcumin ((Sigma, United States), 20 mg of resveratrol (Shanghai yuanye Bio-Technology Co., Ltd) and 160 mg PLGA-PEG (lactide:glycolide 50:50, sigma, United States) were co-dissolved in 5 ml of dichloromethane (Tianjin Damao Chemical Reagent Factory, China) as oil phase (O), 20 ml of PVA (1%, w/w, from sigma, United States) was as external water phase (W). Firstly, the O phase was ultrasonic for 40 s on ice bath to form the first emulsification. Then, the first emulsification was dropped in W phase and ultrasonicated for another 40 s to form the second emulsification. After that, the second emulsification was added to 100 ml water and stirred for 6 h for organic regent evaporation and nanoparticle hardening. Finally, the nanoparticles were harvested by centrifuging at 12,000 rpm for 20 min and washed 3 times using ultrapure water. The harvested NPs were lyophilized for 48 h for storage in powdered form.
4
Pomegranate Peel Bioactive Compounds
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Pomegranate peel was purchased from Hebei Kang’an Biotech. Co. (Anguo, China). p-Nitrophenyl-α-D -glucopyranoside (pNPG) and α-glucosidase (100 U/mg) were obtained from Sigma-Aldrich (Shanghai, China). Acarbose, ellagic acid (EA), and the other compounds including nicotinic acid, isoguanosine, sclareol glycol, triptolide, sclareolide, p-hydroxybenzoic acid, kaempferol, gallic acid, quercetin, and resveratrol were purchased from Yuanye Biotech. Co. (Shanghai, China). All other chemicals used in this study were analytical grade.
5
Bioactive Compounds Extraction and Analysis from Pleioblastus amarus
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The Pleioblastus amarus (Keng) was purchased in Yibin City, Sichuan Province. Fresh shoot shells were dried, crushed, and passed through a 60-mesh sieve. Gallic acid, rutin, p-Coumaric acid, chlorogenic acid, quercetin, catechin, and resveratrol (all High-Performance Liquid Chromatography (HPLC) grade), ABTS, DPPH, 2,4,6-tri (2-pyridyl)–triazine (TPTZ), and Trolox (≥97%) were purchased from Yuanye Biotechnology Co., Ltd., (Shanghai, China). The methanol (HPLC grade, Licrosolv, 99.9%) and acetonitrile (HPLC grade, Licrosolv, 99.9%) were purchased from Semerfeld Technology Co, Ltd. (Shanghai, China), while all other chemicals (analytical grade) were obtained from the Kelong Chemical Reagent Factory (Chengdu, China).
6
Pathogenic R. solanacearum Strain Culture
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The test R. solanacearum strain (Biovar3, Phylotype I) was obtained from Laboratory of Natural Product Pesticide of Southwest university, which was identified to be a highly pathogenic strain. The strain was cultured on NA agar medium (yeast extract 1.0 g, beef extract 3.0 g, peptone 5.0 g, glucose 10.0 g, agar 20.0 g, deionized water 1000 mL, pH 7.0) at 30 °C for 48 h. Single colonies were picked and transferred into NB liquid medium (NA medium without agar) for 12–16 h. The cells were harvested, by centrifugation, at an optical density at 600 nm (OD600) of 1.0 and stored at −80 °C before use.
Resveratrol and coumarin used in this study were purchased from Shanghai Yuanye Bio-technology (Shanghai, China) and had ≥98% purity (by HPLC analysis). Stock solutions (20 mg/mL Resveratrol or coumarin) were prepared in dimethyl sulfoxide (DMSO, Sigma-Aldrich, St. Louis, MO, USA) and filter-sterilized through a bacterial filter before use.
Resveratrol and coumarin used in this study were purchased from Shanghai Yuanye Bio-technology (Shanghai, China) and had ≥98% purity (by HPLC analysis). Stock solutions (20 mg/mL Resveratrol or coumarin) were prepared in dimethyl sulfoxide (DMSO, Sigma-Aldrich, St. Louis, MO, USA) and filter-sterilized through a bacterial filter before use.
7
Quantification of Bioactive Compounds
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Standards of kaempferol, myricetin, chlorogenic acid, caffeic acid, p-coumaric acid, resveratrol, luteolin, gallic acid, and ellagic acid were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). The purity of all standards was required to be greater than or equal to 98%. Potassium oxonate, xanthine oxidase and 2,6-dihydroxypurine were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Xanthine sodium salt (XSS) and allopurinol (APL) were purchased from Sigma Aldrich Inc. (St. Louis, MO, USA). A uric acid (UA) test kit, xanthine oxidase (XOD) assay kit (Colorimetric method), urea assay kit (BUN), and creatinine (Cr) assay kit (sarcosine oxidase) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).
8
Recombinant Protein Expression in E. coli
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E. coli BL21(DE3) cells were purchased from Beijing TransGen Biotech Co., Ltd. (Beijing, China). The Mut Express II Fast Mutagenesis Kit V2 was purchased from Nanjing Vazyme Biotech Co., Ltd. (Nanjing, China). Resveratrol and piceatannol were purchased from Yuanye Biotech Co. Ltd. (Shanghai, China). The Ni-NTA SefinoseTM Resin 6FF (Settled Resin) was purchased from Sangon Co., Ltd. (Shanghai, China). Unless otherwise specified, all chemicals were of analytical grade.
9
Antibiotic Susceptibility Testing with Resveratrol
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Resveratrol was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China) and dissolved in dimethyl sulfoxide (DMSO, ≤2.5% [vol/vol]) (Sigma-Aldrich, Saint Louis, USA). All antibiotics used in this study, including colistin, aztreonam, ceftazidime, cefepime, imipenem, ciprofloxacin, levofloxacin, gentamicin, tobramycin, and amikacin, were purchased from Wenzhou Kangtai Biological Technology Co., Ltd. (Zhejiang, China). Solvents and diluents used to prepare the antibiotic solution complied with the Clinical and Laboratory Standards Institute (CLSI 2020).
10
Resveratrol Cytotoxicity Evaluation in ARPE-19 Cells
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Cell viability was determined using the MTS assay. ARPE-19 cells (5 × 103 cells/well) were seeded in 96-well plates. Resveratrol (10 μg/mL) was purchased from Shanghai Yuanye Bio-Technology and immersed in DMSO. Media containing different concentrations (0 μg/mL, 4 μg/mL, 2 μg/mL, 1 μg/mL, 0.5 μg/mL, 0.25 μg/mL, 0.125 μg/mL, 0.0625 μg/mL, and 0.03125 μg/mL) of the Resveratrol extract and 0.5% DMSO were added and incubated for 24 h. Every dosage was treated using six wells for replicates. Subsequently, a 20 μL mixture of MTS/PMS ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt) (Promega, Madison, WI, USA) 2 mL/phenazine methosulfate 100μL (Sigma-Aldrich, St. Louis, MO, USA) was added and incubated for an additional 2 h. The absorbance was read at 490 nm in the microplate reader SpectraMax® ABS plus (Molecular Devices, San Jose, CA, USA).
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