Olaparib azd2281

A2780 parental (PAR) and A2780 cisplatin resistance daughter clone (CR) cells were provided by Dr Seftor (Northwestern University, Chicago). All the cells lines were authenticated by short tandem repeat (STR) profiling using DNA sequencing. This analysis was performed at the University of Colorado which has extensive experience in evaluation of gynecological cell lines.18 A minimum of 95% of similarity between a reference profile and our cell lines was observed. Cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium, supplemented with 5% fetal bovine serum (FBS), 10mM Hepes and antibiotics (penicillin 100 U/mL, streptomycin 0.1 mg/mL and amphotericin B 0.25 μg/mL) and maintained at 37°C in a humidified incubator with 5% CO₂. Phenformin Hydrochloride and Metformin Hydrochloride were bought from Toronto Research Chemicals. Olaparib (AZD2281) was obtained from AdooQ Bioscience.

To determine 50% inhibitory concentration (IC₅₀) values of cisplatin (cis-dichlorodiammine platinum (II); Sigma-Aldrich), olaparib (AZD2281; Adooq Bioscience, Irvine, CA, USA), and rARL6IP5, we measured the cell proliferation rate using EZ-cytox cell viability kits (DoGenBio Co., Ltd.). IC₅₀ values were analyzed using GraphPad Prism Version 5.0 (San Diego, CA, USA).