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Blimp1 pe

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Blimp1-PE is a fluorescence-labeled antibody reagent used for the detection of Blimp1, a transcriptional repressor involved in the terminal differentiation of B-cells. It is designed for use in flow cytometry applications.

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Lab products found in correlation

Cd3 bv711, by BD (1 mentions) Cd14 bv711, by BD (1 mentions) Igd fitc ia6 2, by BD (1 mentions) Cd38 v450, by BD (1 mentions) Cd138 apc, by Miltenyi Biotec (1 mentions) Cxcr4 pe, by BioLegend (1 mentions) Cxcr3 pe, by BioLegend (1 mentions) Bcma pe, by BioLegend (1 mentions) Cd19 pe cy7, by BD (1 mentions) Facsaria 2, by BD (1 mentions) Ifn γ pe cf594, by BD (1 mentions) Il 2 percp cy5, by BD (1 mentions) Perforin pe cf594, by BD (1 mentions) Fixable viability dye efluor 450, by Thermo Fisher Scientific (1 mentions) Golgiplug, by BD (1 mentions)

2 protocols using blimp1 pe

1

Multiparametric Phenotyping of Human Blood Cells

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Cells were isolated from peripheral blood using Ficoll density gradient centrifugation and perioheral blood mononuclear cells (PBMC) were stained with the following anti-human antibody staining reagents: CD3-BV711 (clone: HIT3a, BD Biosciences); CD3-PE-Cy5.5 (clone: 7D6, Fisher Scientific); CD14-BV711 (clone: M5E2, BD Biosciences); CD14-PE-Cy5.5 (clone: TuK4, Thermo Fisher Scientific); IgD-FITC (IA6-2, BD Biosciences); CD19-PE-Cy7(clone: SJ25C1, BD Biosciences); CD27-APC-eFluor780 (clone: O323, Fisher Scientific); CD38-V450 (clone: HIT2, BD Biosciences); CD138-APC (clone: 44F9, Miltenyi Biotec); CXCR4-PE (clone: 12G5, BioLegend); CXCR4-BV711 (clone:12G5, BD Biosciences); CXCR3-PE(G025H7, BioLegend); Blimp1-PE (6D3, BD Biosciences); BCMA-PE (19F2, BioLegend), IL-6R-PE (M5, BD Biosciences). Approximately, 1 × 103 to 5 × 103 were collected for each cell population by using FACSAria II (BD Biosciences).
Chen W., Hong S.H., Jenks S.A., Anam F.A., Tipton C.M., Woodruff M.C., Hom J.R., Cashman K.S., Faliti C.E., Wang X., Kyu S., Wei C., Scharer C.D., Mi T., Hicks S., Hartson L., Nguyen D.C., Khosroshahi A., Lee S., Wang Y., Bugrovsky R., Ishii Y., Lee F.E, & Sanz I. (2023). SLE Antibody-Secreting Cells Are Characterized by Enhanced Peripheral Maturation and Survival Programs. Research Square.
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2

Polyfunctional T-cell Analysis

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PBMCs were stimulated with anti-CD3/CD28 (2 and 5 μg/mL), plus Golgiplug (BD Pharmingen) for 5 h before intracellular staining Blimp-1-PE, IFN-γ-PE-CF594, and IL-2-PerCp-Cy5.5 (BD Pharmingen). For perforin study, perforin-PE-CF594 (BD Pharmingen) was used. A Fixable Viability Dye eFluor 450 (eBioscience) was used to assess cell viability.
Zhu L., Kong Y., Zhang J., Claxton D.F., Ehmann W.C., Rybka W.B., Palmisiano N.D., Wang M., Jia B., Bayerl M., Schell T.D., Hohl R.J., Zeng H, & Zheng H. (2017). Blimp-1 impairs T cell function via upregulation of TIGIT and PD-1 in patients with acute myeloid leukemia. Journal of Hematology & Oncology, 10, 124.
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