The analytical samples and protocols used in this study were approved by the Ethics Committee of Wuhan Children’s Hospital and Wuhan Maternal and Child Health Hospital (Approval Code: WHCH2020003, Approval Date: 4 February 2020). A written statement that the formal consent of the parent/guardian has been obtained and that the parent/guardian is informed that the study is anonymous. All experiments involving SARS-CoV-2 strains were conducted in a biosafety level 2 (BSL2) laboratory, were approved by the Institute of Microbiology, Chinese Academy of Sciences (IMCAS), and complied with all relevant ethical regulations regarding human research.
The HEK-293T cells line was provided by ATCC CRL-11268. The 293T-ACE2 cell line, the pLenti-GFP lentiviral reporter, plasmids psPAX2, and codon-optimized cDNA encoding SARS-CoV-2 S glycoprotein (QHU36824.1) were obtained from Dr. Zhao Zhendong, Institute of Pathogenic Biology, Chinese Academy of Medical Sciences [15 (link)].
The HEK-293T cells line was provided by ATCC CRL-11268. The 293T-ACE2 cell line, the pLenti-GFP lentiviral reporter, plasmids psPAX2, and codon-optimized cDNA encoding SARS-CoV-2 S glycoprotein (QHU36824.1) were obtained from Dr. Zhao Zhendong, Institute of Pathogenic Biology, Chinese Academy of Medical Sciences [15 (link)].