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Mouse adiponectin elisa kit

Manufactured by Merck Group
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The Mouse Adiponectin ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of mouse adiponectin levels in serum, plasma, and cell culture supernatants. The kit utilizes a specific antibody coated on a microplate to capture the target analyte, and a detection antibody conjugated with an enzyme for signal generation and quantification.

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Lab products found in correlation

Mouse leptin elisa kit, by Merck Group (7 mentions) Rat mouse insulin elisa kit, by Merck Group (4 mentions) Triglyceride assay kit, by Abcam (2 mentions) Mouse rat leptin quantikine elisa, by R&D Systems (2 mentions) Infinite m200 i control, by Tecan (2 mentions) Nesfatin 1 1 82aa rat eia kit, by Phoenix Pharmaceuticals (2 mentions) Nefa fs kit, by DiaSys (2 mentions) Milliplex map mouse adipokine magnetic bead panel, by Merck Group (1 mentions) Dimension xpand plus, by Siemens (1 mentions) Mouse insulin elisa kit, by Merck Group (1 mentions) Nefa c kit, by Fujifilm (1 mentions) Hr series nefa hr 2 kit, by Fujifilm (1 mentions) Free glycerol reagent, by Merck Group (1 mentions) Insulin elisa kit, by Mercodia (1 mentions) Dri chem 3500, by Fujifilm (1 mentions) Triglyceride colorimetric assay kit, by Cayman Chemical (1 mentions) Mouse insulin elisa, by Mercodia (1 mentions) Onetouch ultra blood glucose meter, by LifeScan (1 mentions) Nefa hr kit, by Fujifilm (1 mentions) Infinity triglyceride kit, by Thermo Fisher Scientific (1 mentions)

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Mouse adiponectin ELISA Adiponectin ELISA kit Mouse ELISA kits Adiponectin ELISAs

16 protocols using mouse adiponectin elisa kit

1

Serum Adiponectin Measurement via ELISA

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Serum adiponectin levels were evaluated using the Mouse Adiponectin ELISA Kit (Millipore, Billerica, MA). Serum samples were tested in triplicate.
IGLESIAS D.A., ZHANG Q., CELESTINO J., SUN C.C., YATES M.S., SCHMANDT R.E, & LU K.H. (2016). Lean body weight and metformin are insufficient to prevent endometrial hyperplasia in mice harboring inactivating mutations in PTEN. Oncology, 92(2), 109-114.
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2

Measurement of Metabolic Biomarkers in Mice

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Collected serum samples were sent to Hematology & Biochemistry Clinical Laboratory, Veterinary Laboratory Service Unit, Faculty of Veterinary Medicine, University Putra Malaysia, for measurement of fasting blood glucose, total cholesterol, triglycerides, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) using Dimension Xpand Plus clinical chemistry system (SIEMENS Healthcare Diagnostics Inc.).
Levels of different adipokines (TNF-α, monocyte chemotactic protein 1 (MCP-1), leptin, insulin, interleukin-6 (IL-6) and resistin) in mouse sera were measured using the MILLIPLEX MAP Mouse Adipokine Magnetic Bead Panel (Millipore, Billerica, MA). Serum adiponectin level was measured by commercially available mouse adiponectin ELISA kit, (Millipore, Billerica, MA) according to manufacturer’s protocol.
Khalilpourfarshbafi M., Devi Murugan D., Abdul Sattar M.Z., Sucedaram Y, & Abdullah N.A. (2019). Withaferin A inhibits adipogenesis in 3T3-F442A cell line, improves insulin sensitivity and promotes weight loss in high fat diet-induced obese mice. PLoS ONE, 14(6), e0218792.
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3

Plasma Adiponectin, Leptin, and Liver Triglycerides

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Plasma adiponectin was measured by Mouse adiponectin ELISA kit (Millipore, EZMADP-60K). Leptin was measured by Mouse leptin ELISA kit (Millipore, EZML-82K). Liver triglycerides were measured by Triglyceride Assay Kit (Abcam, ab65336).
Mancini G., Pirruccio K., Yang X., Blücher M., Rodeheffer M, & Horvath T.L. (2019). Mitofusin 2 in Mature Adipocytes Controls Adiposity and Body Weight. Cell reports, 26(11), 2849-2858.e4.
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4

Serum Biomarker Quantification Protocol

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Serum insulin levels were measured by a mouse insulin ELISA kit (Millipore). Serum adiponectin was determined by a mouse adiponectin ELISA kit (Millipore). Serum triglycerides were determined by a kit from Sigma. Serum NEFAs were measured using NEFA C kit (Wako Chemical, Richmond, VA).
Tang P., Virtue S., Goie J.Y., Png C.W., Guo J., Li Y., Jiao H., Chua Y.L., Campbell M., Moreno-Navarrete J.M., Shabbir A., Fernández-Real J.M., Gasser S., Kemeny D.M., Yang H., Vidal-Puig A, & Zhang Y. (2021). Regulation of adipogenic differentiation and adipose tissue inflammation by interferon regulatory factor 3. Cell Death and Differentiation, 28(11), 3022-3035.
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5

Comprehensive Metabolic Profiling in Mice

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Animals were euthanized, and tissues were dissected and flash-frozen in liquid nitrogen. Serum insulin (insulin ELISA kit, Mercodia), leptin (mouse leptin ELISA kit, Millipore), adiponectin (mouse adiponectin ELISA kit, Millipore), NEFAs (HR Series NEFA-HR(2) kit, Wako Diagnostics), and glycerol (free glycerol reagent, Sigma) were measured using commercially available kits. Triglyceride and total cholesterol concentrations were determined by the Van-derbilt Hormone Assay and Analytical Services Core (Vanderbilt University, Nashville, TN). NE, DHPG, serotonin, and 5-HIAA content were measured using high-performance liquid chromatography (HPLC) methods by the Neurochemistry Core Laboratory at the Vanderbilt Brain Institute.
Felsted J.A., Chien C.H., Wang D., Panessiti M., Ameroso D., Greenberg A., Feng G., Kong D, & Rios M. (2017). Alpha2delta-1 in SF1+ Neurons of the Ventromedial Hypothalamus Is an Essential Regulator of Glucose and Lipid Homeostasis. Cell reports, 21(10), 2737-2747.
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6

Oral Glucose Tolerance Test in Mice

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The oral glucose tolerance test (OGTT) was performed at week 20 (after THC treatment for 12 weeks). Mice were fasted for 6 h (morning fast) and orally gavage 1 g/kg glucose. Blood glucose was measured by a OneTouch ultra blood glucose meter (LifeScan, Inc., Milpitas, CA, USA) at 0, 30, 60, 90, and 120 min after administration. The areas under the curve (AUC) of blood glucose levels in each groups were calculated. Fasting serum levels of GOT, GPT, TG, TCHO, and cholesterol/HDL-C were determined using a blood biochemistry analyzer (Fujifilm Dri-Chem 3500s; Fujifilm). Serum insulin (Mercodia Mouse Insulin Elisa, Uppsala, Sweden) was measured by using commercially available ELISA kit. For detection of serum adiponectin (full-length), the serum samples were diluted (1:1000) and assayed by a Mouse Adiponectin ELISA kit according to the manufacturer’s instructions (Millipore; Temecula, CA, USA). The dynamic range of the Mouse Adiponectin ELISA kit is 1–50 ng/mL, and its sensitivity is 0.2 ng/mL. Hepatic TG content was measured by using a Triglyceride Colorimetric Assay Kit (Cayman, Ann Arbor, MI, USA), and the results were expressed as mg TG per gram of liver tissue.
Tsai Y.Z., Tsai M.L., Hsu L.Y., Ho C.T, & Lai C.S. (2021). Tetrahydrocurcumin Upregulates the Adiponectin-AdipoR Pathway and Improves Insulin Signaling and Pancreatic β-Cell Function in High-Fat Diet/Streptozotocin-Induced Diabetic Obese Mice. Nutrients, 13(12), 4552.
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7

Plasma Adiponectin, Leptin, and Liver Triglycerides

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Plasma adiponectin was measured by Mouse adiponectin ELISA kit (Millipore, EZMADP-60K). Leptin was measured by Mouse leptin ELISA kit (Millipore, EZML-82K). Liver triglycerides were measured by Triglyceride Assay Kit (Abcam, ab65336).
Mancini G., Pirruccio K., Yang X., Blücher M., Rodeheffer M, & Horvath T.L. (2019). Mitofusin 2 in Mature Adipocytes Controls Adiposity and Body Weight. Cell reports, 26(11), 2849-2858.e4.
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8

Metabolic Biomarker Measurements

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Insulin were measured by ELISA kit from Alpco, Leptin were measured by ELISA kit from Crystal Chem. Adiponectin was measured with mouse adiponectin ELISA kit (Millipore, Bedford, MA). Serum or plasma triglyceride and cholesterol were measured with an Infinity Triglyceride Kit and Infinity Cholesterol Kit (Thermo Electron Corporation, Vantaa, Finland), respectively. Serum NEFAs were measured with a NEFA-HR kit (Wako Diagnostics, Mountain View, CA). Blood glucose were measured by using Bayer Contour glucometers.
Zhang Z., Funcke J.B., Zi Z., Zhao S., Straub L., Zhu Y., Zhu Q., Crewe C., An Y.A., Chen S., Li N., Wang M.Y., Gaben A.L., Lee C., Gautron L., Engelking L.J., Raj P., Deng Y., Gordillo R., Kusminski C.M, & Scherer P.E. (2021). Adipocyte iron levels impinge on a fat-gut cross-talk to regulate intestinal lipid absorption and mediate protection from obesity. Cell metabolism, 33(8), 1624-1639.e9.
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9

Serum Biomarker Measurement in Mice

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Blood collected from decapitated mice was centrifuged to obtain serum. The serum was transferred to clean vials and stored at -80°C until the day of assay. The corticosterone, leptin, adiponectin, insulin and glucose levels were measured using commercially available kits (Corticosterone EIA kit, Enzo Life Sciences, NY, USA; Mouse leptin ELISA kit, Mouse adiponectin ELISA kit, and Rat/Mouse Insulin ELISA kit, Millipore, Billerica, MA, USA; Glucose assay kit, Calbiochem, San Diego, CA, USA), according to the manufacturer's instructions.
Janštová Ž., Burkuš J., Kubandová J., Fabian D., Koppel J, & Čikoš Š. (2017). The effect of maternal stress on blastocyst quality depends on maternal physiological status. General physiology and biophysics, 36(1).
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10

Quantifying Serum Biomarkers in Mice

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The sera from mice were collected and stored at −80°C and used to quantify the concentration of Nucleobindin-2(Nucb2) in serum using the CUSABIO Mouse Nucb2 Elisa kit (CSB-EL016146MO). ELISAs were read on the Tecan Infinite M200 i-control. Nesfatin-1 was measured using nesfatin-1 (1-82aa) (rat) EIA kit (EK-003-22; Phoenix Pharmaceuticals; Burlingame, CA, USA) with a detectable range of 0.1-1000ng/ml. Adiponectin was measured using Mouse Adiponectin ELISA kit (EMD Millipore; St. Charles, Missouri, USA). Leptin was measured using Mouse/Rat Leptin Quantikine ELISA (R&D Systems; Minneapolis, MN, USA).
Ravussin A., Youm Y.H., Sander J., Ryu S., Nguyen K., Varela L., Suhlman G.I., Sidorov S., Horvath T.L., Schultze J.L, & Dixit V.D. (2018). Loss of Nucleobindin-2 Causes Insulin Resistance in Obesity without Impacting Satiety or Adiposity. Cell reports, 24(5), 1085-1092.e6.
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