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Cxcl16 antibody

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The CXCL16 antibody is a laboratory reagent used for detecting and quantifying the CXCL16 protein in biological samples. CXCL16 is a chemokine involved in immune function and cell signaling. The antibody can be used in various analytical techniques, such as Western blotting, ELISA, and immunohistochemistry, to measure CXCL16 levels in cells, tissues, or body fluids.

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Lab products found in correlation

Nylon mesh filters, by Avantor (2 mentions) Gi254023x, by Merck Group (2 mentions) Live dead fixable aqua stain, by Thermo Fisher Scientific (2 mentions) Ifnγ cytokines, by Thermo Fisher Scientific (2 mentions) Rat igg1κ, by BioLegend (2 mentions) Tnf α, by Thermo Fisher Scientific (2 mentions) Facscanto, by BD (2 mentions)

2 protocols using cxcl16 antibody

1

Investigating CXCL16 Expression on Tumor and Mucosal Cells

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4T1 tumor cells were cultured for 24 hours in media containing 20 ng/mL TNFα and 20 ng/ml IFNγ cytokines (Peprotech) in the presence of 1 μM ADAM10 inhibitor to prevent membrane shedding (GI254023X, Sigma). Cells were dissociated with an enzyme-free dissociation buffer and homogenized to a single cell suspension. Mucosal epithelial cells were harvested directly from a tumor naive BALB/c mouse and were mechanically homogenized and filtered over 70 μm nylon mesh filters (VWR) to obtain a single cell suspension. Isolated cells were stained with a LIVE/DEAD Fixable Aqua stain (Thermo Fisher) and blocked with anti-mouse CD16/CD32 Fc Block (2.4G2) for 10 minutes prior to antibody staining. Cells were stained with either CXCL16 antibody (12–81, BD Biosciences) or isotype control (Rat IgG1κ, Biolegend). All data was acquired on a BD FacsCanto flow cytometer (BD Biosciences) and analyzed using FlowJo software (Treestar).
Christian L.S., Wang L., Lim B., Deng D., Wu H., Wang X.F, & Li Q.J. (2021). Resident memory T cells in tumor-distant tissues fortify against metastasis formation. Cell reports, 35(6), 109118.
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2

Investigating CXCL16 Expression on Tumor and Mucosal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
4T1 tumor cells were cultured for 24 hours in media containing 20 ng/mL TNFα and 20 ng/ml IFNγ cytokines (Peprotech) in the presence of 1 μM ADAM10 inhibitor to prevent membrane shedding (GI254023X, Sigma). Cells were dissociated with an enzyme-free dissociation buffer and homogenized to a single cell suspension. Mucosal epithelial cells were harvested directly from a tumor naive BALB/c mouse and were mechanically homogenized and filtered over 70 μm nylon mesh filters (VWR) to obtain a single cell suspension. Isolated cells were stained with a LIVE/DEAD Fixable Aqua stain (Thermo Fisher) and blocked with anti-mouse CD16/CD32 Fc Block (2.4G2) for 10 minutes prior to antibody staining. Cells were stained with either CXCL16 antibody (12–81, BD Biosciences) or isotype control (Rat IgG1κ, Biolegend). All data was acquired on a BD FacsCanto flow cytometer (BD Biosciences) and analyzed using FlowJo software (Treestar).
Christian L.S., Wang L., Lim B., Deng D., Wu H., Wang X.F, & Li Q.J. (2021). Resident memory T cells in tumor-distant tissues fortify against metastasis formation. Cell reports, 35(6), 109118.
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