4 2 aminoethyl benzenesulfonyl fluoride aebsf

Losartan, lisinopril and AngII were purchased from Wako Pure Chemical (Tokyo, Japan). Benazepril was purchase from Tokyo Chemical Industry (Tokyo, Japan). PD123319, ZD7155, A779, SR202 and EHT1864 were purchased from Tocris Bioscience (Bristol, UK). Diphenylene iodium (DPI) and 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF) were purchased from Sigma-Aldrich. A water-soluble derivative of forskolin, 7-deacetyl-7-[O-(N-methylpiperazino)-γ-butyryl]-forskolin (forskolin-ws), was purchased from Calbiochem (San Diego, CA, USA).
Antagonists of AT1R (losartan and ZD7155) and AT2R (PD123319), the Mas receptor (A779) and PPARγ (SR202) and inhibitors of ACE (benazepril and lisinopril), NOX (DPI and AEBSF) and Rac (EHT1864) were dissolved in the perfused solution and administered to the striatum through the probe during the experimental period. Forskolin-ws, which stimulates cAMP production by activating adenylyl cyclase^{48 (link)}, and AngII were dissolved in sterilized physiological saline (Otsuka Pharmaceutical, Tokyo, Japan) and directly administered into the striatum through the thin needle of the MI-A-I-8-03 probe using an ESP-32 pump. The flow rate was 0.1 μL/min, and the total volume was 1 μL for forskolin-ws and 2 μL for AngII.

The NOX1 inhibitor 4-(2-Aminoethyl)benzenesulfonyl fluoride (AEBSF), the singlet oxygen (¹O₂) scavenger histidine, the NOS inhibitor N-omega-nitro-L-arginine methylester hydrochloride (L-NAME) and the HOCl scavenger taurine were obtained from Sigma-Aldrich (Schnelldorf, Germany).
The peroxynitrite decomposition catalyst 5-, 10-, 15-, 20-Tetrakis(4-sulfonatophenyl)porphyrinato iron(III) chloride (FeTPPS), was obtained from Calbiochem (Merck Biosciences GmbH, Schwalbach/Ts, Germany).
The catalase mimetic EUK-134 [chloro([2,2′-[1,2-ethanediylbis[(nitrilo-κN)methylidyne]]bis[6-methoxyphenolato-κO]]]-manganese was a product of Cayman (Ann Arbor, Michigan, U.S.A.) and was obtained from Biomol (Hamburg, Germany).
Detailed information on inhibitors has been previously published^{74 (link),82 (link)–86 (link)}. The site of action of inhibitors and scavengers has been presented in detail in the Supplementary Material of references #81 and #85.

Fetal calf serum, penicillin/streptomycin, and Trizol reagent were from GIBCO (Grand Island, NY, USA). Poly-L-lysine (mol. wt. > 300,000), trypsin, DNAse, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), propidium iodide (PI), cytochrome c, staurosporine, and 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF) were from Sigma (St. Louis, MO, USA). Dihydroethidium and calcein-AM were purchased from Molecular Probes, Invitrogen (Carlsbad, CA, USA). Mouse anti-actin monoclonal antibodies were from Chemicon Int., anti-β-tubulin monoclonal antibodies were from Sigma-Aldrich, and FITC-goat anti-mouse IgG conjugated were from Zymed Laboratories Inc. All other chemicals were of the purest grade available from regular commercial sources. The colonies of NOX2 (gp91phox) knockout mice and NOX3 knockout mice on a C57BL6 background were purchased from the Jackson Laboratory (Bar Harbor, ME, USA) and were bred in the animal house of our institute.