Sodium octanoate c8

To examine multidrug efflux pumps in E. coli fatty acid salt resistance, antibacterial activities were examined on LB plates containing sodium hexanoate (C6) at concentrations from 78 to 40,000 μg/ml, sodium octanoate (C8) at concentrations from 78 to 40,000 μg/ml, sodium decanoate (C10) at concentrations from 0.63 to 40,000 μg/ml, and sodium dodecanoate (C12) at concentrations from 0.08 to 5,000 μg/ml (Sigma-Aldrich, St Louis, MO, United States; Table 3). Plates were prepared using the 2-fold agar dilution technique (Nishino and Yamaguchi, 2004 (link)). To determine minimum inhibitory concentrations (MICs), bacteria were cultured overnight at 37°C in LB, diluted in the same medium, and tested at a final inoculum of 10⁵ colony forming units/spot using McFarland turbidity standards (Eiken Chemical, Tokyo, Japan) and a multipoint inoculator (Sakuma Seisakusyo, Tokyo, Japan). Plates were incubated at 37°C for 20 h.

Antibacterial activities of various agents were determined on LB agar plates
containing sodium hexanoate (C6), sodium octanoate (C8), sodium decanoate (C10)
and sodium dodecanoate (C12) (Sigma-Aldrich, St Louis, MO, USA) at various
concentrations. Agar plates were prepared using the 2-fold agar dilution
technique [21 (link)]. To
determine minimum inhibitory concentrations (MICs), bacteria were grown in LB at
37°C overnight, diluted with the same medium and then tested at a final inoculum
concentration of 10⁵ cfu/μL using a multipoint inoculator (Sakuma
Seisakusyo, Tokyo, Japan) after incubation at 37°C for 20 h. MIC was the lowest
concentration of the compound required to inhibit cellular growth.