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Isopropyl alcohol ipa

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Isopropyl alcohol (IPA) is a clear, colorless, and flammable liquid. It is a common organic solvent used in various laboratory applications. IPA is an effective cleaning agent and can be used to remove grease, oil, and other contaminants from surfaces. It has a wide range of applications in the scientific and research fields.

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6 protocols using isopropyl alcohol ipa

1

Peptide Synthesis and Microbial Assays

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Mueller-Hinton, Agar SPC, Mueller Hinton Broth (MHB), E. coli ATCC 11775, S. maltophilia ATCC 13636, and S. enteritidis ATCC 13076 were obtained from ATCC (Manassas, VA, USA). BLF, N,N-diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-ethanedithiol (EDT), 4-methylpiperidine, pyridine, and ninhydrin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Rink amide resin, Fmoc-amino acids, 6-chloro-1-hydroxy-benzotriazole (6-Cl-HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropylalcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, MI, USA). All reagents were used without further purification.
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2

Solid-Phase Peptide Synthesis Protocol

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Mueller-Hinton, Agar SPC, E. coli ATCC 25922, and E. faecalis ATCC 29212 were obtained from ATCC, USA. Rink amide resin, Fmoc-Arg(Pbf)-OH, Fmoc-Asp(OtBu)-OH, Fmoc-Lys(Boc)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Gln(Trt)-OH, Fmoc-β-Ala-OH, Fmoc-Phe-OH, Fmoc-Met-OH, Fmoc-Leu-OH, Fmoc-Gly-OH, Fmoc-Ala-OH, Fmoc-Ile-OH, Fmoc-Glu(OtBu)OH, Fmoc-Thr(tBu)-OH, Fmoc-Ser(tBu)-OH, Fmoc-Val-OH, Fmoc-Pro-OH, Fmoc-Cys(Trt)-OH, Fmoc-Lys(Fmoc)-OH, 6-(Fmoc-amino)hexanoic acid (Fmoc-Ahx-OH), 1-hydroxybenzotriazole (HOBt), and N,N-dicyclohexylcarbodiimide (DCC) were purchased from AAPPTec (Louisville, KY, USA). N,N-Diisopropylethylamine (DIPEA), triisopropylsilane (TIPS), 1,2-Ethanedithiol (EDT), 4-methyl-piperidine, pyridine, ninhydrin, phenol, and KCN were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, diethyl ether, N,N-dimethylformamide (DMF), absolute ethanol, dichloromethane (DCM), acetonitrile (ACN), isopropyl alcohol (IPA), and trifluoroacetic acid (TFA) were obtained from Honeywell-Burdick & Jackson (Muskegon, Michigan, USA). All reagents were used without further purification.
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3

Chiral Amine Separation Optimized

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All of chiral analytes tested in this study were purchased from Sigma–Aldrich (St Louis, MO, USA) and Anichem (North Brunswick, NJ, USA). Acetonitrile (ACN), methanol (MeOH), ethanol (EtOH), n-heptane (hept), and isopropyl alcohol (IPA) of HPLC grade were obtained from VWR (Sugarland, Texas), and used as received. Butylamine (BA), acetic acid (AA), trifluoroacetic acid (TFA) and triethylamine (TEA) were obtained from Sigma. Table 1 lists the structures of the 39 racemic primary amines used as test compounds. The chiral amines were sorted according to the four different substituents on their asymmetric center.

Chiral primary amines and the best selectivity value for each.

Table 1Image 1Image 2Image 3Image 4

RR/SS or RS/SR: the amine has two stereogenic centers, hence four possible enantiomers, the indicated enantiomers were considered. Hept: heptane; IPA: isopropyl alcohol; BA: butylamine; ACN: acetonitrile; MeOH: methanol; AA: acetic acid; TEA: triethylamine; EtOH:ethyl alcohol. All mobile phase compositions are given in % v/v.

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4

Synthesis of Functionalized Polyethylene Glycol Amines

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2-(2-(2-Methoxyethoxy)ethoxy)ethylamine was
purchased from Aurum Pharmatech LLC (98% purity), and n-decyl amine was purchased from TCI (>98% purity). N,N′-Disopropylcarbodiimide (DIC) was purchased
from Chem-Impex International, Inc. (≥99.5% purity). 4-Methylpiperidine
was purchased from Beantown Chemical (98% purity). Bromoacetic acid
was purchased from ACROS Organics (≥98% purity). Formic acid
(≥99% purity) was purchased from ThermoFisher Scientific Inc.
Rink amide MBHA resin (0.64 mmol/g) was purchased from Protein Technologies,
Inc. N,N′-Dimethylformamide
(DMF) was purchased from OmniSolv Inc. Trifluoroacetic acid (99% purity)
and N-methylpyrrolidinone (NMP) (99% purity) were
purchased from Sigma-Aldrich. All other needed reagents, 1,2-dichloromethane
(DCM), acetonitrile (ACN), and isopropyl alcohol (IPA), were all purchased
from VWR Chemicals. All chemicals and solvents were used without further
purification.
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5

Thrombin-Binding Aptamer Immobilization Protocol

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3-Aminopropyltriethoxysilane (APTES), α-human thrombin, lyophilized bovine serum albumin (BSA) protein, Phosphate-Buffered Saline (PBS) 10× solution, and 1 M MgCl2 solution were purchased from Fisher Scientific (Waltham, MA, USA). Twenty-five percent glutaraldehyde solution was purchased from Sigma-Aldrich (San Luis, MO, USA). A 15-mer thrombin binding aptamer (TBA) with a sequence of 5’–NH2–(T)12–GGTTGGTGTGGTTGG–3’ was purchased from NZYTech, Lda (Lisbon, Portugal). Absolute ethanol, acetone, 47% hydrofluoric acid (HF) solution, hydrogen peroxide, sulfuric acid, and isopropyl alcohol (IPA) were purchased from VWR (Radnor, PA, USA).
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6

Fabrication of Piezoelectric Polymer Composites

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Prime‐graded silicon (Si) substrate (p‐doped, 100) was purchased from Alpha Nanotech, USA. PDMS kit (SYLGARD 184 Silicone Elastomer) was purchased from the Dow Chemical Company, USA. The piezoelectric 1–3 PZT‐5A composite transducers with a thickness of 200 and 420 µm were purchased from Del Piezo Specialties, USA. The transducers were diced using an ADT 7100 dicing saw (thickness 150 µm), Advanced Dicing Technologies, Israel. Ecoflex 0030 (Part A and B) was purchased from Smooth‐On, USA. The Pyralux copper/polyimide film (Cu/PI of 9 µm/12 µm) and Kapton PI film (thickness: 12, 30, 50, and 100 µm) were purchased from DuPont, USA. The water‐soluble tape 5414 was obtained from 3M, USA. The silver epoxy EPO‐TEK H20E was purchased from Epoxy Technologies, USA. The isopropyl alcohol (IPA) was purchased from VWR International, USA.
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