The gastric tissue (100 mg) was homogenized using a small tissue homogeniser in 40 mM phosphate buffer at pH 7.4. The homogenates were centrifuged at 13,000×g for 10 min at 4 °C. MPO activity in the tissue homogenate was estimated as per the protocol described in the commercial MPO kit (BioVision Incorporated, USA). In brief, supernatants were allowed to react with 5, 5-dithiobis-2-nitrobenzoic acid (DTNB) at 25 °C for 120 min and the reaction was terminated by adding the stop mixture. The change in absorbance was measured at 412 nm using a spectrophotometer.
Mpo kit
Manufactured by Abcam
Sourced in United States
The MPO kit is a laboratory equipment product designed for the detection and analysis of myeloperoxidase (MPO) protein. It provides the necessary reagents and components to perform MPO-related experiments and assays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mpo kit
1
Measuring Gastric Tissue MPO Activity
2
Quantifying Cecal MPO Activity
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