Anti pdpn percp efluor710 nz 1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Anti-PDPN-PerCP-eFluor710 (NZ-1.3) is a fluorescently labeled antibody product used for the detection and analysis of podoplanin (PDPN) in flow cytometry experiments. The antibody is conjugated with the PerCP-eFluor710 fluorescent dye, which can be excited by a 488 nm laser and detected in the 710 nm emission channel.

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Close spelling variants of this product

Anti-PDPN (NZ-1.3, Anti-Pdpn

2 protocols using anti pdpn percp efluor710 nz 1

Synovial Cell Immunophenotyping by Flow Cytometry

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The following antibodies and reagents were used for the analysis of synovial cells with flow cytometry and cell sorting: anti-CD45-APC-H7 (2D1, BD Pharmingen), anti-CD235a-APC-Alexa Fluor750 (11E4B-7-6(KC16), Beckman Coulter), anti-CD31-PE-Cyanine7 (WM-59, eBioscience), anti-CD146-BV450 (P1H12, BD Horizon), anti-CD34-PE (4H11, eBioscience), anti-PDPN-PerCP-eFluor710 (NZ-1.3, eBioscience), anti-THY1-FITC (5E10, BD Pharmingen), anti-cadherin-11-biotin (23C6), human TruStain FcX (BioLegend), streptavidin-APC (Jackson ImmunoResearch), Live/Dead fixable aqua dead cell stain kits (Molecular Probes). For immunofluorescence staining of synovial tissue, following antibodies and reagents were used: anti-CD45 (135-4C5, AbD Serotec), anti-CD34 (EP373Y, Abcam), anti-PDPN (NZ-1.3, eBioscience), anti-THY1 (F15-42-1, Merck Millipore, and clone Thy-1A1, R&D Systems), anti-cadherin-11-Biotin (23C6), anti-Ki67 (16A8, BioLegend), anti-mouse IgG1-FITC (Southern Biotech), anti-mouse IgG2a FITC (Southern Biotech), anti-mouse IgG2b-Alexa Fluor 647 (Life Technologies), anti-rat IgG-Alexa Fluor 594 (Life Technologies), anti-rat IgG-Alexa Fluor 647, anti-rabbit IgG-Alexa Fluor 546 (Life Technologies), Hoechst 33258 (Life Technologies), and anti-FITC Alexa Fluor 488 (Life Technologies).

Mizoguchi F., Slowikowski K., Wei K., Marshall J.L., Rao D.A., Chang S.K., Nguyen H.N., Noss E.H., Turner J.D., Earp B.E., Blazar P.E., Wright J., Simmons B.P., Donlin L.T., Kalliolias G.D., Goodman S.M., Bykerk V.P., Ivashkiv L.B., Lederer J.A., Hacohen N., Nigrovic P.A., Filer A., Buckley C.D., Raychaudhuri S, & Brenner M.B. (2018). Functionally distinct disease-associated fibroblast subsets in rheumatoid arthritis. Nature Communications, 9, 789.

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Synovial Cell Immunophenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies and reagents were used for the analysis of synovial cells with flow cytometry and cell sorting: anti-CD45-APC-H7 (2D1, BD Biosciences, CA, USA), anti-CD235a-APC-Alexa Fluor750 (11E4B-7-6, Beckman Coulter, FL, USA), anti-CD31-PE-Cyanine7 (WM-59, eBioscience, CA, USA), anti-CD146-APC (P1H12, eBioscience), anti-CD34-PE (4H11, eBioscience), anti-PDPN-PerCP-eFluor710 (NZ-1.3, eBioscience), anti-THY1-FITC (5E10, BD Bioscience), anti-CD73-PE-CF594 (AD2, BD Bioscience), anti-CD271-APC (ME20.4, eBioscience), anti-CD54-PE-CF594 (HA58 BioLegend, CA, USA), anti-CD44-APC (G44-26 BD Bioscience), anti-CD29-APC (TS2/16 BioLegend), human TruStain FcX (BioLegend), and Live/Dead fixable aqua dead cell stain kits (Molecular Probes, Thermo Fisher Scientific, MA, USA).

Noda S., Hosoya T., Komiya Y., Tagawa Y., Endo K., Komori K., Koga H., Takahara Y., Sugimoto K., Sekiya I., Saito T., Mizoguchi F, & Yasuda S. (2022). CD34+THY1+ synovial fibroblast subset in arthritic joints has high osteoblastic and chondrogenic potentials in vitro. Arthritis Research & Therapy, 24, 45.

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