Western blotting was performed using the standard method to detect cellular proteins. The antibodies used in this study were as follows: polyclonal rabbit anti-LAPTM5 (Biorbyt, Cat. orb184851,1:1000), monoclonal mouse anti-LAMP1 (Abcam, Cat.ab25630, 1:1000), polyclonal goat anti-gp120 (NIH AIDS Reagent Program, Cat.ARP-288, 1:20000), human monoclonal anti-gp41 (NIH AIDS Reagent Program, Cat.ARP-11557, 1:5000), rabbit anti-GAPDH (Thermo, Cat.PA1-987, 1:1000), mouse monoclonal anti-FLAG (SIGMA, Cat.F1804, 1:1000), rabbit polyclonal anti-p24 (Abcam, Cat.ab63913, 1:1000), mouse monoclonal anti-GFP (Abmart, Cat.M20004, 1:2000), mouse monoclonal anti-HA (Abmart, Cat.M20003, 1:2000), mouse Igs-HRP (Abcam, Cat.6789, 1:5000), Higly cross-Adsorbed Alexa Fluor 488-, 647-, or 555-labeled goat anti-rabbit secondary antibody (Thermo, Cat.A32731, 1:200; Cat.A32733; 1:200; Cat.A32732, 1:200), Highly cross-Adsorbed Alexa Fluor 488-, 647-, or 555-labeled goat anti-mouse secondary antibody (Thermo, Cat.A32723, 1:200; Cat.A32728; 1:200; Cat.A32727, 1:200), rabbit and mouse IgG Trueblot (eBioscience, Cat.18-8816-33,1:1000; Cat.18-8817-33, 1:1000), and rabbit or mouse IgG isotype control (Abcam, Cat.18413; Cat.ab18413).
Mouse monoclonal anti gfp
Manufactured by Abmart
Sourced in China
Mouse monoclonal anti-GFP is a laboratory reagent used to detect and identify the presence of Green Fluorescent Protein (GFP) in samples. It is an antibody produced in mice that specifically binds to and recognizes the GFP protein.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit anti gapdh, by Thermo Fisher Scientific (1 mentions)
Rabbit polyclonal anti p24, by Abcam (1 mentions)
Mouse igs hrp, by Abcam (1 mentions)
Mouse monoclonal anti flag, by Merck Group (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Anti actin, by Abmart (1 mentions)
Protease inhibitor cocktail tablet, by Roche (1 mentions)
Gallocatechin, by Merck Group (1 mentions)
Epigallocatechin gallate (egcg), by Merck Group (1 mentions)
Catechin, by Merck Group (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Proteintech (1 mentions)
Mouse monoclonal anti gst, by Proteintech (1 mentions)
3 protocols using mouse monoclonal anti gfp
1
Comprehensive Western Blotting Antibody Protocols
2
Analyzing Protein Expression in Baculovirus-Infected Sf9 Cells
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Sf9-L cells (1 × 106 cells/35-mm-diameter dish) were infected with vAcWT at a multiplicity of infection (MOI) of 10. At the indicated time points p.i., the cells were washed twice with phosphate-buffered saline (PBS) and collected via centrifugation at 1,000 × g for 10 min. The pelleted cells were lysed on ice for 15 min in radio-immunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl [pH 7.6], 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate [SDS]; Thermo Scientific) supplemented with protease inhibitor cocktail tablets (one tablet/25 ml; Roche) and then mixed with an equal volume of 2 × protein sample buffer. Equal volumes of each sample were resolved by SDS-10% PAGE, electrophoretically transferred to polyvinylidene difluoride membranes (Millipore), and probed with mouse monoclonal anti-GFP (1:2,000; Abmart) or anti-actin (1:2,000; Abmart) antibodies according to the manufacturer’s instructions. Specific proteins were detected with a goat anti-mouse horseradish peroxidase (HRP)-conjugated antibody (1:5,000; Pierce) and enhanced chemiluminescence reagents (ECL; Amersham Biosciences) on standard X-ray film (Kodak).
3
Phytochemical Profiling of Specialty Crops
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Tea (C. sinensis L. cv. ‘Shuchazao’), oil tea (C. oleifera Abel.), grape (V. vinifera L.), and persimmon (D. kaki Thunb.) plants were cultivated in the experimental garden at Anhui Agricultural University (Anhui, China). Fresh samples were collected, rapidly frozen in liquid nitrogen, and stored at −80°C until use. Nicotiana tabacum cv. G28 (tobacco) and N. benthamiana were grown in a greenhouse with a light intensity of 150–200 μmol m−2 sec−1, a photoperiod of 16/8 h light/dark, and a temperature of 22 ± 2°C.
Authentic standard compounds, EC, EGC, ECG, EGCG, PGG, catechin, and gallocatechin were purchased from Sigma‐Aldrich (St. Louis, MO, USA). 1‐O‐β‐Glucogallin and caffeoyl glucoside were biosynthesized with CsUGT84A22 according to the methods in our previous report (Cui et al., 2016 (link)). EC‐Caf was provided by Prof. Guanhu Bao. Mouse monoclonal anti‐GFP was purchased from Abmart Inc. (Shanghai, China). Rabbit polyclonal anti‐plant actin was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Mouse monoclonal anti‐GST, mouse monoclonal anti‐MBP, and horseradish peroxidase‐conjugated secondary antibodies were purchased from Proteintech, Inc. (Wuhan, China).
Authentic standard compounds, EC, EGC, ECG, EGCG, PGG, catechin, and gallocatechin were purchased from Sigma‐Aldrich (St. Louis, MO, USA). 1‐O‐β‐Glucogallin and caffeoyl glucoside were biosynthesized with CsUGT84A22 according to the methods in our previous report (Cui et al., 2016 (link)). EC‐Caf was provided by Prof. Guanhu Bao. Mouse monoclonal anti‐GFP was purchased from Abmart Inc. (Shanghai, China). Rabbit polyclonal anti‐plant actin was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Mouse monoclonal anti‐GST, mouse monoclonal anti‐MBP, and horseradish peroxidase‐conjugated secondary antibodies were purchased from Proteintech, Inc. (Wuhan, China).
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