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Plexiglass chamber

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The Plexiglass chamber is a transparent enclosure made of durable acrylic material. It is designed to provide a controlled environment for various applications, such as cell culture, plant growth, and other scientific experiments. The chamber's primary function is to create a contained and isolated space that can be precisely regulated in terms of temperature, humidity, and atmospheric conditions.

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Lab products found in correlation

C57bl 6 mice, by Charles River Laboratories (2 mentions) Hydrocortisone, by Pfizer (2 mentions) C57bl 6n mice, by Charles River Laboratories (1 mentions)

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Plexiglas chamber (9 citations)

7 protocols using plexiglass chamber

1

Postnatal Hyperoxia and Hydrocortisone in Mice

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This study was approved by the Institutional Animal Care and Use Committee at Northwestern University. Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth (10 (link),40 (link)). Dams were rotated every 24 hours between normoxia and hyperoxia cages to prevent toxicity. Pups received one of three doses of hydrocortisone (1 mg/kg, 5 mg/kg, or 10 mg/kg) (Pfizer, New York, NY) subcutaneously every other day or equivalent volume of vehicle (sterile water) for 14d. The pups were euthanized after 14d of exposure.
Perez M., Wisniewska K., Lee K.J., Cardona H.J., Taylor J.M, & Farrow K.N. (2016). Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury. Pediatric research, 79(5), 759-765.
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2

Hyperoxia Exposure in SOD3 Knockout Mice

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This study was approved by the Northwestern University Institutional Animal Care and Use Committee. SOD3−/− mice (The Jackson Laboratory, Bar Harbor, ME, USA) were bred on a background of C57Bl/6N mice (Charles River, Wilmington, MA, USA) and back-crossed through multiple generations. Heterozygotes were then crossed to produce SOD3−/− (KO) and SOD3+/+ (WT) offspring. Cages were checked daily for litters. New litters were placed in either room air (RA, 21% FiO2) or a plexiglass chamber (Biospherix, Lacona, NY, USA) at 75% FiO2 (Hyperoxia, or O2) from birth to P14 continuously. Dams were rotated daily to prevent oxygen toxicity. Mice were euthanized at 14 days.
Mathias M., Taylor J., Mendralla E, & Perez M. (2021). Neonatal Extracellular Superoxide Dismutase Knockout Mice Increase Total Superoxide Dismutase Activity and VEGF Expression after Chronic Hyperoxia. Antioxidants, 10(8), 1236.
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3

Postnatal Hyperoxia and Hydrocortisone in Mice

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This study was approved by the Institutional Animal Care and Use Committee at Northwestern University. Aged-matched C57Bl/6 mice (Charles River, Wilmington, MA) were placed in room air (normoxia) or 75% O2 (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) within 24h of birth (10 (link),40 (link)). Dams were rotated every 24 hours between normoxia and hyperoxia cages to prevent toxicity. Pups received one of three doses of hydrocortisone (1 mg/kg, 5 mg/kg, or 10 mg/kg) (Pfizer, New York, NY) subcutaneously every other day or equivalent volume of vehicle (sterile water) for 14d. The pups were euthanized after 14d of exposure.
Perez M., Wisniewska K., Lee K.J., Cardona H.J., Taylor J.M, & Farrow K.N. (2016). Dose-Dependent Effects of Glucocorticoids on Pulmonary Vascular Development in a Murine Model of Hyperoxic Lung Injury. Pediatric research, 79(5), 759-765.
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4

Postnatal Oxygen-Induced Lung Injury Model

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The animal protocol was approved by the Institutional Animal Care and Use Committee at UC Davis. Timed-pregnant Sprague Dawley dams at E14-E16 were ordered from Charles River Laboratories (Wilmington, MA). Rats were housed in plastic cages with a 12h dark:light cycle and allowed to feed ad libitum with a standard diet (2018 Teklad from Harlan). After birth, pups were pooled and randomly assigned to litters of 10 pups (control) or 17 pups (PNGR). Additionally, pups were randomly assigned to cages maintained in room air or exposed to 75% oxygen in a plexiglass chamber (Biospherix, Lacona, NY) continuously, and dams were rotated with the appropriate control or PNGR dam every 24h. In a second experiment, pups in each group were treated either with 5X106 cfu Lactobacillus reuteri DSM 17938 (Protectis Biogaia, Sweden) or with phosphate-buffered saline (PBS) daily by gavage at a maximum safe volume of 10μl/g body weight. Pups treated with L. reuteri were cohoused with PBS treated pups. At postnatal day 14, the pups were analyzed by echocardiography, weighed and euthanized for tissue harvest. Hearts, lungs and intestines were snap-frozen in liquid nitrogen and stored at −80°C.
Wedgwood S., Warford C., Agvatisiri S.R., Thai P.N., Chiamvimonvat N., Kalanetra K.M., Lakshminrusimha S., Steinhorn R.H., Mills D.A, & Underwood M.A. (2019). The developing gut-lung axis: post-natal growth restriction, intestinal dysbiosis and pulmonary hypertension in a rodent model. Pediatric research, 87(3), 472-479.
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5

Postnatal Oxygen-Induced Lung Injury Model

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The animal protocol was approved by the Institutional Animal Care and Use Committee at UC Davis. Timed-pregnant Sprague Dawley dams at E14-E16 were ordered from Charles River Laboratories (Wilmington, MA). Rats were housed in plastic cages with a 12h dark:light cycle and allowed to feed ad libitum with a standard diet (2018 Teklad from Harlan). After birth, pups were pooled and randomly assigned to litters of 10 pups (control) or 17 pups (PNGR). Additionally, pups were randomly assigned to cages maintained in room air or exposed to 75% oxygen in a plexiglass chamber (Biospherix, Lacona, NY) continuously, and dams were rotated with the appropriate control or PNGR dam every 24h. In a second experiment, pups in each group were treated either with 5X106 cfu Lactobacillus reuteri DSM 17938 (Protectis Biogaia, Sweden) or with phosphate-buffered saline (PBS) daily by gavage at a maximum safe volume of 10μl/g body weight. Pups treated with L. reuteri were cohoused with PBS treated pups. At postnatal day 14, the pups were analyzed by echocardiography, weighed and euthanized for tissue harvest. Hearts, lungs and intestines were snap-frozen in liquid nitrogen and stored at −80°C.
Wedgwood S., Warford C., Agvatisiri S.R., Thai P.N., Chiamvimonvat N., Kalanetra K.M., Lakshminrusimha S., Steinhorn R.H., Mills D.A, & Underwood M.A. (2019). The developing gut-lung axis: post-natal growth restriction, intestinal dysbiosis and pulmonary hypertension in a rodent model. Pediatric research, 87(3), 472-479.
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6

Hypoxia Exposure Effects in Mice

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Cultures treated with the appropriate conditions were placed in humidified airtight incubation chambers (Billups-Rothenberg, Del Mar, CA) which were gassed with 1% O2, 5% CO2, and balance N2. For the duration of the specified hypoxia exposures the incubation chambers were kept inside a 37°C incubator. Normoxic cells were kept in a tissue culture incubator maintained at 5% CO2 and 37°C.
Male C57/bl6 mice at 8 weeks of age (n = 5) were randomized to normoxic or hypoxic exposure. Hypoxic mice were exposed to 10% O2 in a plexiglass chamber (Biospherix, Inc.) monitored by Pro:Ox oxygen controller (model 350; Reming Bioinstruments, Redfield, NY), as previously described [16 (link)]. Designated groups were administered with 3mg/kg olomoucine in 1% DMSO or vehicle via an intraperitoneal injection 24 hours before exposure to hypoxia, and another dose 4 hours prior to hypoxia exposure.
Kim B.S., Serebreni L., Fallica J., Hamdan O., Wang L., Johnston L., Kolb T., Damarla M., Damico R, & Hassoun P.M. (2015). Cyclin-Dependent Kinase Five Mediates Activation of Lung Xanthine Oxidoreductase in Response to Hypoxia. PLoS ONE, 10(4), e0124189.
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7

Neonatal Hyperoxia Exposure and Recovery

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Litters of vehicle and IUGR pups were placed in either 75% oxygen (hyperoxia) in a Plexiglass chamber (Biospherix, Lacona, NY) or 21% oxygen (room air) within 24 hours after birth for 14 days [35 (link)], [36 (link)]. Exposure to hyperoxia was continuous, with brief interruption only for animal care (<10 minutes/day). The concentration of oxygen was maintained with an oxygen controller (ProOx, Biospherix). Ventilation within the chamber was adjusted to remove CO2 such that it did not exceed 0.5%. A hygro-thermometer was used in the chamber to monitor temperature and humidity. Temperature in the chamber did not exceed 23°C and humidity level was maintained using dishes of desiccant in the bottom of the chamber. A foster dam was placed in the hyperoxia chamber with each vehicle or IUGR litter, and foster dams were rotated from hyperoxia to room air every 24–48 hours to prevent excessive oxygen toxicity to the adult animals. The litters were removed from the hyperoxia chamber at 14 days and allowed to recover in room air until day of life 28. The pups continued to be fostered until weaned at 21 days.
Chang J.L., Bashir M., Santiago C., Farrow K., Fung C., Brown A.S., Dettman R.W, & Dizon M.L. (2018). Intrauterine growth restriction and hyperoxia as a cause of white matter injury. Developmental neuroscience, 40(4), 344-357.
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