Total RNA was isolated from tissue and cells using RNA-Bee reagent (Amsbio) and was reverse transcribed into cDNA using iSCRIPT (BioRad). Reactions were performed on the LightCycler 480 II (Roche) using 0.4 uM primers and Faststart Universal SYBR Green PCR Master Mix (Applied Biosystems). Primer sequences are described in Supplementary Table S1 .
Faststart universal sybr green pcr master mix
Manufactured by Thermo Fisher Scientific
Sourced in United States
Faststart Universal SYBR Green PCR Master Mix is a ready-to-use solution designed for real-time PCR amplification. It contains all the necessary components, including SYBR Green I dye, for conducting quantitative PCR (qPCR) reactions.
Automatically generated - may contain errors
Lab products found in correlation
Iscript, by Bio-Rad (2 mentions)
Lightcycler 480 2, by Roche (2 mentions)
Rna bee reagent, by AMS Biotechnology (2 mentions)
Reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Ez press rna purification kit, by EZBioscience (1 mentions)
Lightcycler 480 2 system, by Roche (1 mentions)
Nanodrop nd 2000 spectrometer, by Thermo Fisher Scientific (1 mentions)
3 protocols using faststart universal sybr green pcr master mix
1
Quantitative RT-PCR for Gene Expression
2
RNA Isolation and qRT-PCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA from harvested cells or tissues was isolated by EZ-press RNA Purification Kit (EZBioscience, Roseville, CA, USA). The purity and quality of RNA were measured by a NanoDrop ND-2000 spectrometer (NanoDrop Technologies, Wilmington, DE, USA). Then, the first strand of cDNA was synthesized using a reverse transcription kit (Thermo Fisher Scientific, Waltham, MA, USA). qRT-PCR was then performed with FastStart Universal SYBR Green PCR Master mix (Applied Biosystems, Waltham, MA, USA) using a LightCycler 480 II System (Roche Diagnostics GmbH, Mannheim, Germany). The gene-specific primers are listed in Table . Glyceraldehyde 3-phosphate dehydrogenase as used as the endogenous control for each sample. The relative target mRNA expression was calculated using the following equation: relative expression = 2 [∆Ct(control)–∆Ct(target)].
3
Quantitative RT-PCR for Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was isolated from tissue and cells using RNA-Bee reagent (Amsbio) and was reverse transcribed into cDNA using iSCRIPT (BioRad). Reactions were performed on the LightCycler 480 II (Roche) using 0.4 uM primers and Faststart Universal SYBR Green PCR Master Mix (Applied Biosystems). Primer sequences are described in Supplementary Table S1 .
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