Plastic 96 well plates were coated with purified Der p1 and blocked with 1% bovine serum albumin dissolved in PBS. Recombinant human CD163 (R&D Systems, Inc., Minneapolis, MN) (120 ng) was added to the Der p1-coated plates overnight, washed 3 times with 0.05% Tween 20 in PBS, and the quantity of CD163 that bound to the immobilized Der p1 was quantified using a CD163 immunoassay kit (R&D Systems, Inc., Minneapolis, MN) with a horseradish peroxidase conjugated anti-CD163 antibody. Non-specific binding of the anti-CD163 antibody to Der p1-coated plates in the absence of CD163, as well as background related to the immobilized Der p1 protein in the absence of the anti-CD163 antibody were subtracted from the reported values.
Recombinant human cd163
Manufactured by R&D Systems
Recombinant human CD163 is a soluble protein produced in HEK293 cells. It corresponds to the extracellular domain of the human CD163 protein.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using recombinant human cd163
1
Quantification of Der p1-CD163 Binding
2
Quantification of Der p1-CD163 Binding
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Plastic 96 well plates were coated with purified Der p1 and blocked with 1% bovine serum albumin dissolved in PBS. Recombinant human CD163 (R&D Systems, Inc., Minneapolis, MN) (120 ng) was added to the Der p1-coated plates overnight, washed 3 times with 0.05% Tween 20 in PBS, and the quantity of CD163 that bound to the immobilized Der p1 was quantified using a CD163 immunoassay kit (R&D Systems, Inc., Minneapolis, MN) with a horseradish peroxidase conjugated anti-CD163 antibody. Non-specific binding of the anti-CD163 antibody to Der p1-coated plates in the absence of CD163, as well as background related to the immobilized Der p1 protein in the absence of the anti-CD163 antibody were subtracted from the reported values.
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