Axiocam mrm monochrome digital camera

Manufactured by Zeiss

The Axiocam MRm is a monochrome digital camera designed for microscopy applications. It features a high-resolution sensor and advanced imaging capabilities to capture detailed, high-quality microscopic images. The camera's core function is to provide a reliable and accurate means of recording and documenting microscopic samples and specimens.

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Lab products found in correlation

Axiovert 200m microscope, by Zeiss (2 mentions) Fluoromount g, by Southern Biotech (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Prolong gold mounting media, by Thermo Fisher Scientific (1 mentions) Ec plan neofluar 100 1.3 oil ph3 objective, by Zeiss (1 mentions) Axio imager z1 microscope, by Zeiss (1 mentions) Axiovision, by Zeiss (1 mentions) Aqua poly mount, by Polysciences (1 mentions)

Close spelling variants of this product

AxioCam MRm (847 citations) AxioCam MRm camera (708 citations) AxioCam (641 citations) AxioCam camera (328 citations) AxioCam digital camera (134 citations) Digital camera (68 citations) AxioCam MRm digital camera (60 citations) AxioCam digital (18 citations) MRm camera (16 citations) AxioCam MRm monochrome camera (11 citations)

3 protocols using axiocam mrm monochrome digital camera

Immunofluorescence Microscopy Protocol

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Immunofluorescence procedures were carried out with antibodies diluted in DMEM media supplemented with 10% FBS (as a blocking agent) and 0.1% saponin (as a permeabilizing agent) [25 (link),30 (link),33 (link)]. Briefly, upon a 15 min fixation with 4% formaldehyde, cells were incubated with primary antibodies for 1 h at room temperature. Secondary antibodies with fluorescent probe conjugation were incubated for 45 min at room temperature in the dark. DAPI was used to label the nucleus, and coverslips were mounted on glass slides using Fluoromount-G (0100-01, SouthernBiotech, Birmingham, AL, USA).
All fluorescence imaging was carried out with a Zeiss Axiovert 200 M microscope along with a Zeiss Axiocam MRm monochrome digital camera and the Carl Zeiss Axiovision image acquisition software (version 4.4) [25 (link),30 (link),33 (link)]. Random fields of the entire coverslip were imaged, and exposure times for each channel were kept consistent within independent experiments. Total magnification for images were kept at 400× using the Zeiss Objective EC “Plan-Neofluar” 40×/0.75 M27.

Lee J.J., Ramadesikan S., Black A.F., Christoffer C., Pacheco A.F., Subramanian S., Hanna C.B., Barth G., Stauffacher C.V., Kihara D, & Aguilar R.C. (2023). Heterogeneity in Lowe Syndrome: Mutations Affecting the Phosphatase Domain of OCRL1 Differ in Impact on Enzymatic Activity and Severity of Cellular Phenotypes. Biomolecules, 13(4), 615.

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Centriole Proximity Imaging Assay

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hTERT-RPE1 and HFF cells were grown on glass coverslips. Cells were fixed as described with either cold 100% methanol for 5 min, 4% formaldehyde for 10 min, or methanol/acetone (1:1) for 2 min. Cells fixed with formaldehyde were permeabilized with 0.2% Triton X-100 in PBS for 10 min. Before fixation and cilia staining, cells were incubated on ice for 30 min to depolymerize microtubules. Fixed cells were blocked in blocking buffer (2.5% FBS, 0.1% Triton X-100 in PBS) for 1 h and then incubated with primary antibodies for 1 h at room temperature, followed by 1-h incubation with secondary antibodies (Alexa Fluor 488, 594, and 647; Thermo Fisher Scientific). DNA was stained with Hoechst 33342 (Thermo Fisher Scientific) for 2 min. Coverslips were mounted on glass slides using ProLong Gold mounting media (Thermo Fisher Scientific). Cells were imaged with AxioVision software on an inverted Axiovert 200M microscope (NA: 0.55, working distance: 26 mm; ZEISS) equipped with an EC Plan-Neofluar 100×/1.3 oil Ph3 objective. Images were captured with an AxioCam MRm monochrome digital camera (ZEISS). The distance between centrioles was measured using AxioVision software.

Loukil A., Tormanen K, & Sütterlin C. (2017). The daughter centriole controls ciliogenesis by regulating Neurl-4 localization at the centrosome. The Journal of Cell Biology, 216(5), 1287-1300.

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Immunofluorescence Staining of iPSC and HK2 Cells

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All cells were grown on coverslips and fixed with 4% formaldehyde/PBS for 10min at room temperature. After washing with PBS, the iPSC cells were incubated with the corresponding primary antibody diluted in blocking solution (10% FBS containing DMEM medium with 0.1% saponin) at 4°C, overnight while HK2 cells (WT and OCRL1 KO) were stained with specific primary antibodies (see S1 Table) for 1h at room temperature, Following two washes with PBS, the cells were incubated with appropriate secondary antibodies diluted in blocking solution for 45min at room temperature. The cells were washed and stained with DAPI for 5min, washed and mounted on slides with Aqua-PolyMount (Polysciences).
Images were acquired using a Zeiss Axioimager.Z1 microscope equipped with Zeiss Axiocam MRm monochrome digital camera and Carl Zeiss Axiovision image acquisition software.

Hsieh W.C., Ramadesikan S., Fekete D, & Aguilar R.C. (2018). Kidney-differentiated cells derived from Lowe Syndrome patient’s iPSCs show ciliogenesis defects and Six2 retention at the Golgi complex. PLoS ONE, 13(2), e0192635.

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