Ecs 400 400 mhz nmr spectrometer

All reactions were carried out under nitrogen unless indicated otherwise. All reagents were obtained from available commercial sources and were used without further purification unless otherwise noted. The silica gel used in flash chromatography was 60 Å, 230–400 mesh. Analytical TLC was performed on Uniplate 250 µm silica gel plates with detection by UV light. NMR spectra were acquired on a JEOL ECS-400 400 MHz NMR spectrometer with multinuclear capability and 24-sample autosampler, with solvent as internal reference; the chemical shifts are reported in ppm, in δ units. Infrared spectra were acquired on a Nicolet Avatar FTIR. Ultraviolet-visible spectroscopy experiments were conducted on a Shimadzu UV-2450 spectrometer fitted with a TCC-240A temperature-controlled cell chamber. HPLC experiments were conducted using an Agilent 1200 system with a degasser, photodiode array detector, and temperature-controlled autosampler. High resolution mass spectroscopic data were obtained at the Mass Spectrometry & Analytical Proteomics Laboratory at the University of Kansas (Lawrence, KS). Regression analyses were completed using the GraphPad Prism 6 software suite.

All reactions were carried out under nitrogen unless indicated otherwise. All reagents were obtained from available commercial sources and were used without further purification unless otherwise noted. The silica gel used in flash chromatography was 60 Å, 230–400 mesh. Analytical TLC was performed on Uniplate 250 µm silica gel plates with detection by UV light. NMR spectra were acquired on a JEOL ECS-400 400 MHz NMR spectrometer with multinuclear capability and 24-sample autosampler, with solvent as internal reference; the chemical shifts are reported in ppm, in δ units. Infrared spectra were acquired on a Nicolet Avatar FTIR. Ultraviolet-visible spectroscopy experiments were conducted on a Shimadzu UV-2450 spectrometer fitted with a TCC-240A temperature-controlled cell chamber. HPLC experiments were conducted using an Agilent 1200 system with a degasser, photodiode array detector, and temperature-controlled autosampler. High resolution mass spectroscopic data were obtained at the Mass Spectrometry & Analytical Proteomics Laboratory at the University of Kansas (Lawrence, KS). Regression analyses were completed using the GraphPad Prism 6 software suite.

All reactions were carried out under nitrogen unless indicated otherwise. All reagents were obtained from available commercial sources and were used without further purification unless otherwise noted. Melting point analyses were conducted using an open capillary tube, unless otherwise noted. The silica gel used in flash chromatography was 60 A, 230–400 mesh. Analytical TLC was performed on Uniplate 250 pm silica gel plates with detection by UV light. NMR spectra were acquired on a JEOL ECS- 400 400 MHz NMR spectrometer with multinuclear capability and 24- sample autosampler, with solvent as internal reference; the chemical shifts are reported in ppm, in δ units. Infrared spectra were acquired on a Nicolet Avatar FTIR. High resolution mass spectroscopic data were obtained at the Mass Spectrometry & Analytical Proteomics Laboratory at the University of Kansas (Lawrence, KS). Cell viability absorption wasmeasured using a SpectraMax M2 Multi-Mode Microplate Reader. Firefly luciferase activity was measured using a Glomax 96-well Luminometer (Promega). RNA integrity was determined using an Agilent 2100 Bioanalyzer. Quantitative real-time PCR analysis was conducted using an Applied Biosystems 7500 Real Time PCR System.