L-cysteine, γ-benzyl-L-glutamate ((Bzl)Glu), ɛ-Z-L-lysine ((Z)Lys), L-leucine (Leu), triphosgene, α-pinene, trifluoromethanesulfonic acid (TFMSA), trifluoroacetic acid (TFA), 2-(p-tolyl)-pyridine (NCtpy) and other reagents were purchased from Sigma–Aldrich (Germany) and used as received. 1,4-Dioxane, n-hexane were purchased from Vecton Ltd. (Russia) and distilled prior to use. Dimethyl sulfoxide (DMSO), also purchased from Vecton Ltd. (Russia), was dried under molecular sieves 4 Ǻ and distilled under vacuum. Ethyl acetate purchased from Panreac (Spain) was distilled before application. All solvents were purified and distilled using standard procedures.
HEK 293 (human embryonic kidney cells), HELF (human embryonic lung fibroblasts) and U937 (human lymphoblast lung cells) cell lines were purchased from BioloT (Russia). The cells were cultured in Dulbecco’s Modified Eagle Medium (BioloT, Russia) and grown in Dulbecco’s Modified Eagle’s Medium containing 10% (v/v) heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, UT, USA), 1% L-glutamine, 1% sodium pyruvate, 50 U/mL penicillin and 50 μg/mL streptomycin (BioloT).
HEK 293 (human embryonic kidney cells), HELF (human embryonic lung fibroblasts) and U937 (human lymphoblast lung cells) cell lines were purchased from BioloT (Russia). The cells were cultured in Dulbecco’s Modified Eagle Medium (BioloT, Russia) and grown in Dulbecco’s Modified Eagle’s Medium containing 10% (v/v) heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, UT, USA), 1% L-glutamine, 1% sodium pyruvate, 50 U/mL penicillin and 50 μg/mL streptomycin (BioloT).