Cells were enumerated using Perfect-Count Microspheres™ (Cytognos, Salamanca, Spain) microbeads in a Navios EX flow cytometer (Beckman Coulter, Pasadena, CA, USA). Viability (%) was determined using the 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, Pasadena, CA, USA) exclusion method. Data were analyzed with Navios EX Software v2.0 (Beckman Coulter) software. In accordance with de International Society on Cell and Gene Therapy (ISCT) criteria [3 (link)], identity of MSC was evaluated by the expression of surface markers CD31 PacificBlue-conjugated (clone 5.6E; Beckman Coulter), CD45 KromeOrange-conjugated (clone J33; Beckman Coulter), CD73 PE-conjugated (clone AD-2; Beckman Coulter), CD90 FITC-conjugated (clone F15-42–1-5, Beckman Coulter), CD105 PC7-conjugated (clone TEA3/17.1.1; Beckman Coulter), and HLA-DR APC-conjugated (clone Immu-357; Beckman Coulter) in a Navios EX Device. Cells were stained for 15 min at room temperature, washed and resuspendend with DPBS. Acquisition was done using Navios EX, and raw data were analyzed with Navios EX Software (version 2.0, Beckman Coulter).
Cd105 pc7 conjugated clone tea3 17.1.1
Manufactured by Beckman Coulter
CD105 PC7-conjugated (clone TEA3/17.1.1) is a fluorochrome-conjugated monoclonal antibody used for the detection and analysis of CD105-positive cells in flow cytometry applications. The antibody is conjugated with the PC7 fluorochrome, which allows for detection in the appropriate channel of a flow cytometer.
Automatically generated - may contain errors
Lab products found in correlation
Navios ex, by Beckman Coulter (2 mentions)
Navios ex software v2, by Beckman Coulter (2 mentions)
Cd31 pacificblue conjugated clone 5.6e, by Beckman Coulter (2 mentions)
Cd45 kromeorange conjugated clone j33, by Beckman Coulter (2 mentions)
Cd73 pe conjugated clone ad 2, by Beckman Coulter (2 mentions)
Cd90 fitc conjugated clone f15 42 1 5, by Beckman Coulter (2 mentions)
Navios ex software, by Beckman Coulter (2 mentions)
Perfect count microspheres, by Cytognos (2 mentions)
7 aminoactinomycin d, by Beckman Coulter (2 mentions)
2 protocols using cd105 pc7 conjugated clone tea3 17.1.1
1
Characterization of Mesenchymal Stem Cells by Flow Cytometry
2
Characterization of Mesenchymal Stem Cells by Flow Cytometry
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Cells were enumerated using Perfect-Count Microspheres™ (Cytognos, Salamanca, Spain) microbeads in a Navios EX flow cytometer (Beckman Coulter, Pasadena, CA, USA). Viability (%) was determined using the 7-Amino-Actinomycin D (7-AAD, Beckman Coulter, Pasadena, CA, USA) exclusion method. Data were analyzed with Navios EX Software v2.0 (Beckman Coulter) software. In accordance with de International Society on Cell and Gene Therapy (ISCT) criteria [3 (link)], identity of MSC was evaluated by the expression of surface markers CD31 PacificBlue-conjugated (clone 5.6E; Beckman Coulter), CD45 KromeOrange-conjugated (clone J33; Beckman Coulter), CD73 PE-conjugated (clone AD-2; Beckman Coulter), CD90 FITC-conjugated (clone F15-42–1-5, Beckman Coulter), CD105 PC7-conjugated (clone TEA3/17.1.1; Beckman Coulter), and HLA-DR APC-conjugated (clone Immu-357; Beckman Coulter) in a Navios EX Device. Cells were stained for 15 min at room temperature, washed and resuspendend with DPBS. Acquisition was done using Navios EX, and raw data were analyzed with Navios EX Software (version 2.0, Beckman Coulter).
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