Antibody against idh1

Manufactured by R&D Systems

The Antibody against IDH1 is a laboratory reagent used to detect the presence of the IDH1 protein in biological samples. IDH1 is an enzyme involved in cellular metabolism. This antibody can be used in various research applications such as Western blotting, immunohistochemistry, and immunocytochemistry to study the expression and localization of IDH1 in different cell and tissue types.

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Lab products found in correlation

Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit igg h l secondary antibody, by Thermo Fisher Scientific (2 mentions) P67phox, by Santa Cruz Biotechnology (1 mentions) Anti nox2 antibody, by Abcam (1 mentions) Antibody against ha, by Santa Cruz Biotechnology (1 mentions)

2 protocols using antibody against idh1

Antibody Characterization for Immunoblotting

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Antibodies against DYKDDDDK (FLAG) tag, p-Tyrosine (p-Tyr-100) were from Cell Signaling Technology (CST). Antibodies against β-actin and were from Sigma-Aldrich. Antibody against IDH1 was from R&D SYSTEMS. Antibodies against SHP2, PTPMT1 and PTP1B was from PROTEINTECH. Goat anti-Mouse IgG (H+L) secondary antibody and goat anti-rabbit IgG (H+L) secondary antibody were from Thermo Fisher Scientific. Antibodies against p-IDH1 Y42 and p-IDH1 Y391 were custom-made by SHANGHAI GENOMICS, INC. Anti-NOX2 antibody was purchased from Abcam and Bioss. p67phox was purchased from Santa Cruz. Anti-rabbit IgG (H+L) F(ab')2 Fragment (Alexa Fluor 488 conjugate) and Anti-mouse IgG (H+L) F(ab')2 Fragment (Alexa Fluor 555 conjugate) was purchase from Cell Signaling Technology.

Zhang R., Chen D., Fan H., Wu R., Tu J., Zhang F.Q., Wang M., Zheng H., Qu C.K., Elf S.E., Faubert B., He Y.Y., Bissonnette M.B., Gao X., DeBerardinis R.J, & Chen J. (2022). Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells. Cell chemical biology, 29(7), 1200-1208.e6.

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Antibody Characterization for Protein Analysis

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Antibodies against DYKDDDDK (FLAG) tag, p-Tyrosine (p-Tyr-100), Jak2, p-Jak2 (Tyr1007/1008), di-methyl-histone H3 (Lys9), histone H3, FLT3 and Src were from Cell Signaling Technology (CST). Antibodies against β-actin and p-Src (Tyr418) were from Sigma-Aldrich. Antibody against HA was from Santa Cruz Biotechnology. Antibody against trimethyl (Lys9) was from Abcam. Antibodies against IDH1-R132H and Trimethyl-Histone H3 (Lys4) were from EMD Millipore. Antibody against IDH1 was from R&D SYSTEMS. Antibody against PE-Cy^™5 Mouse Anti-Human CD235a was from BD Pharmingen^™. Goat anti-Mouse IgG (H+L) secondary antibody and goat anti-rabbit IgG (H+L) secondary antibody were from Thermo Fisher Scientific. Antibodies against p-IDH1 Y42 and p-IDH1 Y391 were custom-made by SHANGHAI GENOMICS, INC. Antibodies against Ki67 and IDH1 for IHC staining studies were from Abcam.

Chen D., Xia S., Wang M., Lin R., Li Y., Mao H., Aguiar M., Famulare C.A., Shih A.H., Brennan C.W., Gao X., Pan Y., Liu S., Fan J., Jin L., Song L., Zhou A., Mukherjee J., Pieper R.O., Mishra A., Peng J., Arellano M., Blum W.G., Lonial S., Boggon T.J., Levine R.L, & Chen J. (2019). Mutant and wild-type isocitrate dehydrogenase 1 share enhancing mechanisms involving distinct tyrosine kinase cascades in cancer. Cancer discovery, 9(6), 756-777.

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