Dp72 cooled ccd camera

UV-vis spectra were recorded using Cary 50 Bio UV-vis spectrophotometer and zeta potentials were determined by Brookhaven 90Plus nanoparticle size analyzer with BI-Zeta module. Attenuated total reflectance-Fourier Transform infrared spectroscopy (ATR-FTIR) was carried out with PerkinElmer Spectrum 100. Fluorescence spectroscopy was measured using QuantaMaster Model C-60/2000 spectrofluorimeter at the excitation wavelength of 295 nm to minimize tyrosine fluorescence. Scanning electron microscopy and transmission electron microscopy were performed with Hitachi S-4800 and FEI Tecnai T-12 respectively. Olympus fluorescence microscope BX 53 coupled with mercury-vapor short arc lamp (X-cite series 120Q) as light source and LMPlanFL N 20x lens was used to record images of β-LG intrinsic fluorescence on Olympus DP72 cooled CCD camera with 330 and 420 nm excitation and emission filter sets at an exposure time of 60 s. A pH Meter (Accumet Research AR50) was used to monitor the pH adjustments by 1 M HCl or NaOH.