Cd45 pacific blue

Manufactured by Beckman Coulter

The CD45 Pacific blue is a fluorochrome-conjugated monoclonal antibody that binds to the CD45 antigen, a transmembrane glycoprotein expressed on the surface of most human leukocytes. It is designed for use in flow cytometry applications to identify and quantify various leukocyte populations.

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Lab products found in correlation

Cd69 fitc, by BD (1 mentions) Cd31 pe, by BD (1 mentions) Cd56 apc, by BD (1 mentions) Il 17 pe, by Thermo Fisher Scientific (1 mentions) Cd4 fitc, by BioLegend (1 mentions) Cd19 apc cy7, by BioLegend (1 mentions) Cd4 bv421, by BioLegend (1 mentions) Ifn γ fitc, by BD (1 mentions) Il 2 pe, by BioLegend (1 mentions) Cd107a pe, by BD (1 mentions) Cd4 pe cy7, by Beckman Coulter (1 mentions) Cd21 pe cy7, by BioLegend (1 mentions) Cd21 pe cy7, by BD (1 mentions) Vα24 pe, by Beckman Coulter (1 mentions) Vβ11 fitc, by Beckman Coulter (1 mentions) Ccr7 pe, by R&D Systems (1 mentions) Il 4 apc, by Thermo Fisher Scientific (1 mentions) Igm alexa fluor 647, by Jackson ImmunoResearch (1 mentions) Bcl 6, by Leica (1 mentions) Flag tag (flag), by Santa Cruz Biotechnology (1 mentions)

2 protocols using cd45 pacific blue

Comprehensive Immune Cell Phenotyping

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The following antibodies were used in this study: CD3 AF700, CD4 BV421, CD4 FITC, CD6 APC, CD10 BV605, CD19 APC-Cy7, CD21 PE-Cy7, CD25 PerCP-Cy5.5, CD27 BV421, CD28 PerCP-Cy5.5, CD38 PerCp-Cy5.5, CD45RA APC-Cy7, TCR αβ PerCP-Cy5.5, IL-2 PE, LAT PE, NTAL/LAB APC, and biotin anti–human TCR-Vd2 (BioLegend); TCR-Vd1 APC (Miltenyi Biotec); CD8 APC, CD8 Pacific blue, CD21 PE-Cy7, CD31 PE, CD56 APC, CD69 FITC, CD107a PE, CD127 Alexa Fluor 647, IFN-γ FITC, TCR γδ PE, IgG Alexa Fluor 700, IκBα PE, ERK1/2(pT202/pY204) AF647, and ZAP70(pY319)/SYK(pY352) APC (BD); IgD FITC and IgA PE (SouthernBiotech); CD3 PE-Cy7, CD4 PE-Cy7, CD8 PE, CD16 FITC, CD45RA FITC, CD45 Pacific blue, Vα24 PE, and Vβ11 FITC (Beckman Coulter); CCR7 PE (R&D Systems); Bruton tyrosine kinase/ITK(pY551/pY511) PE, IL-17 PE, IL-4 APC, and ICOS PE (eBioscience); IgM Alexa Fluor 647 (Jackson ImmunoResearch Laboratories, Inc.); and PLCγ1(pY783) and goat anti–rabbit AF647 (Cell Signaling Technology). For immunohistochemistry, CD21 (Dako), CD20 (Invitrogen), CD3 (Cell Marque), CD4 and CD8 (Spring Bioscience), and Bcl-6 (Leica Biosystems) were used. For immunoblotting LAT (sc-7948; Santa Cruz Biotechnology, Inc.), FLAG tag (AHP1074; AbD Serotec), actin (sc-1616; Santa Cruz Biotechnology, Inc.), PLCγ1 (pY783; no. 2821; Cell Signaling Technology), and ZAP70 (pY319; no. 2701; Cell Signaling Technology) were used.

Keller B., Zaidman I., Yousefi O.S., Hershkovitz D., Stein J., Unger S., Schachtrup K., Sigvardsson M., Kuperman A.A., Shaag A., Schamel W.W., Elpeleg O., Warnatz K, & Stepensky P. (2016). Early onset combined immunodeficiency and autoimmunity in patients with loss-of-function mutation in LAT. The Journal of Experimental Medicine, 213(7), 1185-1199.

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Comprehensive Cytometric Analysis of T Cell Subsets

Cited 1 time

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Flow cytometry was performed using the following antibodies from BioLegend, unless otherwise stated. Antibodies to the following human molecules were used: CD69-AF647 (FN50), CD69-PE (FN50), CD3-BV421 (OKT3), γδTCR-PeCy7 (IMMU510; Beckman Coulter), CD45-PacificBlue (HI30), TCRVδ2-FITC (B6). Antibodies to the following murine molecules were used: TCRδ-PerCPe710 (GL3), TCRδ-APC (GL3), Vγ7-AF647 (F2.67, provided by P.Pereira). Other antibodies were as follows: DYKDDDDK-PE (FLAG, L5), HA-AF647 (16B12). 6xHis-APC (Biolegend, J095G46). Data were acquired on BD Canto II or Fortessa cytometers. sTCR staining was performed as in Melandri et al. (2018) (link).

Willcox C.R., Vantourout P., Salim M., Zlatareva I., Melandri D., Zanardo L., George R., Kjaer S., Jeeves M., Mohammed F., Hayday A.C, & Willcox B.E. (2019). Butyrophilin-like 3 Directly Binds a Human Vγ4+ T Cell Receptor Using a Modality Distinct from Clonally-Restricted Antigen. Immunity, 51(5), 813-825.e4.

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