Ml171

The complete NCI-60 panel was obtained from National Cancer Institute (NCI) Anticancer Drug Screen (Bethesda, MD, USA). Pancreatic cell lines were obtained from ATCC (Manassas, VA, USA). All cells were cultivated in RPMI including 2 mM glutamine and 11.11 mM glucose supplemented by 10% FBS and penicillin/streptomycin (GIBCO, Grand Island, NY, USA).
Romidepsin (depsipeptide, NSC 630176) was provided by the Cancer Therapy Evaluation Program, NCI. Tariquidar was provided by the NCI Anticancer Drug Screen. PD-0325901 (MEK inhibitor), MK-2206 (AKT inhibitor) were purchased from ChemieTek (Indianapolis, IN, USA). Glutaminase inhibitor 968 compound was obtained from Millipore (Burlington, MA, USA); oligomycinA, NAC, glutathione, dimethyl-α-ketoglutarate, dimethyl-glutamate, sodium citrate, and sodium acetate were obtained from Sigma-Aldrich (St. Louis, MO, USA); DPI, ML171, and etomoxir was obtained from Selleckchem (Houston, TX). MitoQ was provided by Dan L. Sackett (NIH, NICHD).
Unless otherwise specified, t-tests were performed to evaluate significance of results, and asterisks were added to graphs when the p-value was lower than 0.05.

Restriction enzymes for molecular cloning were purchased from New England BioLab (New England Biolabs, Ipswich, MA, USA). KOD-Plus-Neo PCR kit was purchased from Toyobo (Osaka, Japan). ClonExpress Ultra One Step Cloning Kit was purchased from Vazyme Biotech (Vazyme Biotech, Nanjing, China; C115). Plasmid mini prep kit was purchased from Tiangen (TIANGEN, Beijing, China; DP103). For cell cultures, DMEM medium was purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA), FBS was purchased from Hyclone (GE Healthcare Life Sciences, Chicago, IL, USA), Lipofectamine 3000 transfection reagent kit was purchased from Invitrogen (Invitrogen, Carlsbad, CA, USA). For ROS detection assays, HBSS was purchased from Gibco (Thermo Fisher Scientific), BSA was purchased from Mpbio (MP Biomedicals, Irvine, CA, USA), isoluminol, HRP and PMA were purchased from Sigma-Aldrich (St. Louis, MO, USA). For NOX1 inhibition, ML171 was purchased from Selleck Chemicals LLC (Houston, TX, USA; S5304), VAS2870 was purchased from Sigma-Aldrich (St. Louis, MO, USA; SML0273), GKT137831 was purchased from Selleck Chemicals LLC (Houston, TX, USA; S7171).