Cell death in the hippocampal region was assessed using the immunofluorescent terminal deoxynucleotidyl transferase‐mediated deoxyuridine triphosphate nick‐end labeling (TUNEL) technique (kit G3250, Promega, Madison, WI, USA). The slides were mounted with Vectashield mounting solution with 4′, 6′‐diamidino‐2‐phenylindole dihydrochloride hydrate (DAPI; H‐1200; Vector) and visualized by 20× (dentate gyrus) and 5× tile‐scan confocal microscopy (Leica, Wetzlar, Germany). A blinded evaluator counted the density of TUNEL‐positive nuclei in whole brain on coronal brain sections. Six coronal sections (+0.95 mm to −0.11 mm/bregma) were counted from each brain.
Kit g3250
Manufactured by Promega
Sourced in United States
Kit G3250 is a laboratory product offered by Promega. It is a kit designed for a specific purpose, but no further details about its core function or intended use can be provided in an unbiased and factual manner without the risk of extrapolation.
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Lab products found in correlation
3 protocols using kit g3250
1
Quantifying Neuronal Apoptosis in Hippocampus
2
TUNEL Assay for Brain Cell Death
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According to the manufacturer’s protocol, brain cell death was assessed using terminal transferase-mediated biotin dUTP nick end labeling (TUNEL) assay (kit G3250, Promega, Madison, WI, USA). We blindly evaluated the number of TUNEL positive cells using three non-overlapping fields in three coronal sectioned brains (+0.95 mm to −0.11 mm/bregma) in the peri-infarct area of each brain.
3
Quantifying Brain Cell Death via TUNEL
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Brain cell death was evaluated via a terminal transferase-mediated biotin dUTP nick end labeling (TUNEL) assay (kit G3250, Promega, Madison, WI, USA), according to the manufacturer’s protocol. TUNEL positive cells were counted in a blinded manner on three non-overlapping fields in three coronal sectioned brains (+0.95 mm to −0.11 mm/bregma) in the peri-infarct area of each brain.
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