Zen lite 2012 analysis software

Manufactured by Zeiss

Sourced in Germany

ZEN lite 2012 is a microscope image analysis software developed by Zeiss. It provides core functionality for image acquisition, processing, and analysis.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Formaldehyde solution, by Merck Group (1 mentions) Lsm 510 meta confocal microscope, by Zeiss (1 mentions)

Spelling variants of this product

ZEN software (2479 citations) ZEN 2012 software (475 citations) ZEN lite software (176 citations) ZEN lite (78 citations) ZEN lite 2012 (42 citations) Zen analysis software (12 citations) ZEN 2012 lite software (10 citations)

4 protocols using zen lite 2012 analysis software

Visualizing Actin Networks in Root Hair Development

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F-actin networks at root hair initiation sites were visualised in seedlings expressing GFP-FABD, GFP-ABD2-GFP, Lifeact-Venus or in seedlings probed with BODIPY FL phallacidin (Nishimura et al., 2003 (link)). For display, images of early trichoblast cells acquired on longitudinal cross-sections at distances of 0.52 µm (GFP-FABD or Lifeact-Venus), 0.91 µm (GFP-ABD2-GFP) or 0.45 µm (BODIPY FL phallacidin) from each other, respectively, were processed by maximum intensity projection, employing ZEN lite 2012 analysis software (Zeiss).
To visualise cell file-specific AIP1-2-mCherry expression, longitudinal sections at a distance of 3.54 µm from a top view of the analysed seedling root were processed by maximum-intensity projection, employing ZEN lite 2012 analysis software. Cross-sectional overviews were generated by transversally projecting selected areas of 15.1 µm in length into one image.

Kiefer C.S., Claes A.R., Nzayisenga J.C., Pietra S., Stanislas T., Hüser A., Ikeda Y, & Grebe M. (2015). Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity. Development (Cambridge, England), 142(1), 151-161.

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Quantifying Lipid Content in Spheroids

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The NileRed lipid stain (Sigma-Aldrich) was used for fluorescence microscopy. Spheroids were fixed in 4% formaldehyde solution (Sigma-Aldrich) at room temperature for one hour and thereafter washed three times with PBS. Next, spheroids were incubated in 2 µM NileRed stain in PBS together with 1 µg/ml Hoechst 33342 (Thermo Fisher Scientific, USA) O/N at room temperature. Before imaging, spheroids were again washed three times with PBS. All fluorescent images were acquired using an LSM710 confocal microscope (Zeiss, Germany) and images were processed with ZEN lite 2012 analysis software (Zeiss, Germany). The intensity of neutral lipid staining was quantified and normalized for number of nuclei per spheroid using CellProfiler software.

Kozyra M., Johansson I., Nordling Å., Ullah S., Lauschke V.M, & Ingelman-Sundberg M. (2018). Human hepatic 3D spheroids as a model for steatosis and insulin resistance. Scientific Reports, 8, 14297.

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Visualizing CCR5 Expression Using Antibody Staining

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JC53 cells expressing high CCR5 levels were first incubated with a CCR5 N-terminus antibody (purified mouse anti-human CCR5, clone T21/8, BioLegend) or CCR5 ECL2 mAb (mouse monoclonal IgG2b, clone 45531, R&D Systems), or both antibodies in combination, at 100 µg/ml (N-terminus) or 50 µg/ml (ECL2) for 2 h at room temperature. We also tested a multi epitope CCR5 primary antibody, anti-human CCR5 mAb, clone 45523 (Endres et al., 1996 (link)) (NIH AIDS Research and Reference Reagent Program, Germantown, MD), which was incubated on the cells in the same manner. Samples were then washed and incubated with a fluorescent FLSC IgG1 Zenon Alexa 488 complex for 1 h in the dark at room temperature. The fluorescent FLSC IgG1 complex was created using a Zenon Alexa 488 human IgG kit (Invitrogen) following manufacturer protocols. Cell-associated fluorescence was visualized using a Zeiss META LSM 510 confocal microscope and inhibition of staining with FLSC IgG1 Zenon Alexa 488 by CCR5 mAbs was analyzed using ZEN Lite 2012 analysis software (Carl Zeiss).

Latinovic O., Schneider K., Szmacinski H., Lakowicz J.R., Heredia A, & Redfield R.R. (2014). Binding of fusion protein FLSC IgG1 to CCR5 is enhanced by CCR5 antagonist Maraviroc. Antiviral research, 112, 80-90.

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Confocal Imaging with Zeiss LSM710

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All fluorescent images were acquired using an LSM710 confocal microscope (Zeiss, Germany). Images were processed with ZEN lite 2012 analysis software (Zeiss, Germany).

Bell C.C., Hendriks D.F., Moro S.M., Ellis E., Walsh J., Renblom A., Fredriksson Puigvert L., Dankers A.C., Jacobs F., Snoeys J., Sison-Young R.L., Jenkins R.E., Nordling Å., Mkrtchian S., Park B.K., Kitteringham N.R., Goldring C.E., Lauschke V.M, & Ingelman-Sundberg M. (2016). Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease. Scientific Reports, 6, 25187.

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