Opn4-/- and age-matched wild-type mice were maintained on normal chow for three days on either a 14h:10h red LD or 14h:10h blue LD cycle in Oxymax metabolic chambers (Columbus Instruments, Columbus, OH). After this “habituation run”, animals were returned to polystyrene cages under the same LD cycle where they remained on normal chow for four days. On the eighth day, animals were returned to the metabolic chambers under their designated LD cycle and were introduced to the previously described ketogenic diet. This period of time was designated as the “data run” and lasted for three days. Food and water intake, activity, oxygen consumption and carbon dioxide production were simultaneously monitored. Respiratory exchange ratios and heat production were automatically calculated from these parameters. Only the data from the last three days were used for statistical comparisons (t-tests).
Oxymax metabolic chambers
Manufactured by Columbus Instruments
Sourced in United States
The Oxymax metabolic chambers from Columbus Instruments are designed to measure the oxygen consumption and carbon dioxide production of small laboratory animals. The chambers provide a controlled environment to monitor the metabolic activity of the subjects.
Automatically generated - may contain errors
6 protocols using oxymax metabolic chambers
1
Metabolic Adaptation to Ketogenic Diet
Check if the same lab product or an alternative is used in the 5 most similar protocols
Opn4-/- and age-matched wild-type mice were maintained on normal chow for three days on either a 14h:10h red LD or 14h:10h blue LD cycle in Oxymax metabolic chambers (Columbus Instruments, Columbus, OH). After this “habituation run”, animals were returned to polystyrene cages under the same LD cycle where they remained on normal chow for four days. On the eighth day, animals were returned to the metabolic chambers under their designated LD cycle and were introduced to the previously described ketogenic diet. This period of time was designated as the “data run” and lasted for three days. Food and water intake, activity, oxygen consumption and carbon dioxide production were simultaneously monitored. Respiratory exchange ratios and heat production were automatically calculated from these parameters. Only the data from the last three days were used for statistical comparisons (t-tests).
+ Expand
Opn4-/- and age-matched wild-type mice were maintained on normal chow for three days on either a 14h:10h red LD or 14h:10h blue LD cycle in Oxymax metabolic chambers (Columbus Instruments, Columbus, OH). After this “habituation run”, animals were returned to polystyrene cages under the same LD cycle where they remained on normal chow for four days. On the eighth day, animals were returned to the metabolic chambers under their designated LD cycle and were introduced to the previously described ketogenic diet. This period of time was designated as the “data run” and lasted for three days. Food and water intake, activity, oxygen consumption and carbon dioxide production were simultaneously monitored. Respiratory exchange ratios and heat production were automatically calculated from these parameters. Only the data from the last three days were used for statistical comparisons (t-tests).
2
Molecular Modulation of Gonadal Function
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anaesthetized with isofluorane and received analgesics (0.01mg/mL buprenorphine, 0.58mg/mL carprofen) pre- and post- surgery. Bilateral ovariectomy and castration surgery with complete removal of the ovaries or the testes was performed on adult mice. For Figure 4 , sham or gonadectomized control mice (XXF and XYM) and gonadectomized FCG mice from separate experimental batches are shown together. The Cre-dependent AAV8-hM3Dq-mCherry DREADD (Addgene, titer ≥ 4×1012 vg/mL, 200 nL to each side) was injected bilaterally into the VMHvl of adult female mice (coordinates: A-P: −1.56 mm from Bregma; lateral: ±0.85 mm from Bregma; D-V: 5.6 mm from the cortex). After 2 weeks of recovery, mice received i.p. injections of CNO (0.3 mg/kg) or vehicle (saline, 0.15% DMSO) 3 hr after the onset of the light phase. Saline and CNO were administered on consecutive days in a randomized balanced design. siRNA pools against Rprm or non-targeting controls (Dharmacon, 0.4 mM, 350 nL to each side) were delivered to the VMHvl as described above. Indirect calorimetry was performed in Oxymax metabolic chambers (Columbus Instruments) at room temperature. Gross movement and core body temperature were measured using an IP-implanted G2 eMitter and VitalView software (Starr Life Sciences).
3
Molecular Modulation of Gonadal Function
4
Metabolic Response to LPS Challenge
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Energy expenditure was measured using the Oxymax Metabolic Chambers (Columbus Instruments, Columbus, OH, United States). HIF-1DF and HIF-1DF/LysM mice were randomly allocated to the chambers where they had free access top food and water. Baseline VO2, VCO2, and RER readings were recorded for 48 h but the data for the initial 24 h was discarded. Once mice had acclimatized to the chamber, LPS (15 mg/kg) was administered by I.p. injection and recordings were obtained for a further 6 h.
5
Comprehensive Metabolic Profiling of NC-Fed Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Oxygen consumption, carbon dioxide production, food and water consumption, and ambulatory movement were determined in NC-fed male mice using the metabolic chambers (Oxymax metabolic chambers, Columbus Instruments).
6
Comprehensive Metabolic Profiling of Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For determination of energy expenditure and respiratory exchange ratios, mice were placed in Oxymax metabolic chambers (Columbus Instruments), under a constant environmental temperature (22 °C) and a 12hour light, 12-hour dark cycle as previously described (15) . Oxygen consumption (VO2), carbon dioxide production (VCO2), and ambulatory activity were determined for each mouse every 15 min over a 72-h period.
Experimental mice were acclimated to the cages during the first 24 hours. Measurements taken during the following 48 hours were used for data analysis. Respiratory exchange ratios (RER) were calculated as carbon dioxide production/oxygen consumption (VCO2/VO2). Carbohydrate utilization was calculated as (20 kJ/L × VO2 uptake) × [(RER -0.7)/0.3] and fat utilization was calculated as (20kJ/ × VO2uptake) × (1 -[(RER × 0.7)/0.3)] (16). Both body fat and lean mass were included as covariates in the models used to analyze data obtained from the CLAMS experiments. Mice in each chamber had free access to water and food. Locomotor activity was monitored by a multidimensional infrared light beam system surrounding each cage.
Experimental mice were acclimated to the cages during the first 24 hours. Measurements taken during the following 48 hours were used for data analysis. Respiratory exchange ratios (RER) were calculated as carbon dioxide production/oxygen consumption (VCO2/VO2). Carbohydrate utilization was calculated as (20 kJ/L × VO2 uptake) × [(RER -0.7)/0.3] and fat utilization was calculated as (20kJ/ × VO2uptake) × (1 -[(RER × 0.7)/0.3)] (16). Both body fat and lean mass were included as covariates in the models used to analyze data obtained from the CLAMS experiments. Mice in each chamber had free access to water and food. Locomotor activity was monitored by a multidimensional infrared light beam system surrounding each cage.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!