1 4 dithiotreitol

Reduced GSH, sodium hypochlorite, L-methionine, and 1,4-dithiotreitol were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals were of analytical grade. All experiments were performed in 145 mM potassium phosphate buffer pH 7.4.

All chemicals were of analytical grade, if not otherwise stated. Sodium chloride (NaCl), sodium hydroxide (NaOH), SDS, 2‐(N‐moprholino)ethanesulfate acid (MES), Tween‐20, sulfuric acid (95–97%, H₂SO₄), uranyl acetate were purchased from Merck (Darmstadt, Germany).
4‐(2‐Hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES) (≥99.5%), 2‐propanol, bovine serum albumin (BSA) (≥99.5%), 1,4‐dithiotreitol (DTT), anti‐mouse IgG (γ‐chain specific)‐alkaline phosphatase antibody (#3438), BCIP^®/NBT solution, triton X‐100, glutaraldehyde solution (grade I), acetonitrile (MS grade), formic acid (98–100%), and iodacetamide (≥99%) were purchased from Sigma Aldrich (St. Louis, MO, USA). Anti‐rabbit IgG (H+L) secondary antibody (#31460), anti‐mouse IgG (H+L) superclonal secondary antibody (#A28177) were purchased from Thermo Fisher (Waltham, MA, USA). SeeBlue^® plus 2 prestained protein standard and 4× LDS sample buffer were purchased from Invitrogen (Carlsbad, CA, USA). C‐LEcta Denarase^® was purchased from VWR (Radnor, PA, USA), HIV‐1 p24 antibody (ab9071) and ACV5 (ab49581) from Abcam (Cambridge, UK), influenza A virus H1N1 HA (GTX127357) from GeneTex (Irvine, CA, USA) and trypsin from Promega (Madison, WI, USA).