Agilent 6495 triple quadrupole

Manufactured by Agilent Technologies

The Agilent 6495 Triple Quadrupole is a mass spectrometry instrument designed for sensitive and accurate quantitative analysis. It features a triple quadrupole configuration, which allows for precise fragmentation and detection of analytes. The core function of the Agilent 6495 is to provide high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) capabilities for a variety of analytical applications.

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Lab products found in correlation

Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions) 1290 infinity 2 uplc system, by Agilent Technologies (1 mentions) Agilent 1290 infinity 2 uplc system, by Agilent Technologies (1 mentions)

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Agilent 6495 triple quadrupole mass spectrometer (13 citations) Agilent 6495 (6 citations) 6495 Triple Quadrupole (4 citations)

2 protocols using agilent 6495 triple quadrupole

Quantitative Glycerol-d8 and Propanediol-d8 Analysis

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Detailed methods are provided in Supplementary Methods. Briefly, using deuterated glycerol-d8 [Sigma. 447498] and 6 (±)-1,2-propanediol-d8 [CDN, D-1656]) as an internal standard, samples were analyzed by LCMS/MS (Agilent 1290 Infinity II UPLC system with Agilent Zorbax Eclipse Plus C18 column; Santa Clara, CA). Eluted compounds were further separated and quantified through the coupled Agilent 6495 Triple Quadrupole equipped with an electrospray ion (ESI) source. The coefficient of variation for PG from 14-blinded repeat samples was 15.4%.

Song M.A., Reisinger S.A., Freudenheim J.L., Brasky T.M., Mathé E.A., McElroy J.P., Nickerson Q.A., Weng D.Y., Wewers M.D, & Shields P.G. (2019). Effects of Electronic Cigarette Constituents on the Human Lung: A Pilot Clinical Trial. Cancer prevention research (Philadelphia, Pa.), 13(2), 145-152.

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Nicotine Metabolite and Propylene Glycol Analysis

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Trans-3 -hydroxycotinine (3HC) and cotinine (COT) were analysed as previously reported by liquid chromatography-tandem mass spectrometry (LC-MS/MS).³¹ Urine creatinine was used to normalize 3HC and COT levels, and then molar sums were calculated for the nicotine equivalent combination using 3HC +COT as 2 nicotine metabolites (2NE).³⁹
Propylene Glycol (PG) was measured with deuterated 6 (±)-1,2-propanediol-d8 [CDN, D-1656]) as an internal standard and then analysed by LC-MS/MS (Agilent 1290 Infinity II UPLC system).³¹ The coupled Agilent 6495 Triple Quadrupole in addition to an electrospray ion (ESI) source was used to separate and quantify the eluted compound.

Mori K.M., McElroy J.P., Weng D.Y., Chung S., Fadda P., Reisinger S.A., Ying K.L., Brasky T.M., Wewers M.D., Freudenheim J.L., Shields P.G, & Song M.A. (2022). Lung mitochondrial DNA copy number, inflammatory biomarkers, gene transcription and gene methylation in vapers and smokers. eBioMedicine, 85, 104301.

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