Bone marrow MSCs were transfected with a miR-21a-5p inhibitor, NC inhibitor, miR-21a-5p mimic, or NC mimic at a concentration of 150 nM using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. After 48 h of transfection, qPCR was performed to assess the expression of miR-21a-5p. The miR-21a-5p inhibitor, NC inhibitor, miR-21a-5p mimic, and NC mimic were synthesized by GenePharma (Shanghai, China) using the following sequences: miR-21a-5p inhibitor, 5′-UCAACAUCAGUCUGAUAAGCUA-3′; and NC inhibitor, 5′-CAGUACUUUUGUGUAGUACAA-3′; miR-21a-5p mimic: sense, 5′-UAGCUUAUCAGACUGAUGUUG-3′ and antisense, 5′-AACAUCAGUCUGAUAAGCUAUU-3′; NC mimic: sense, 5′-UUCUCCGAACGUGUCACGUTT-3′ and antisense, 5′-ACGUGACACGUUCGGAGAATT-3′.
Mir 21a 5p mimic
Manufactured by GenePharma
Sourced in China
MiR-21a-5p mimics are synthetic RNA molecules designed to mimic the function of the naturally occurring miR-21a-5p microRNA. MiR-21a-5p is involved in various biological processes, including cell proliferation, apoptosis, and gene regulation. The mimics are used in research and experimental settings to study the effects of increased miR-21a-5p expression.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
Mimic nc, by GenePharma (1 mentions)
Pmirglo stat3 wt 3 utr, by GenePharma (1 mentions)
Pmirglo stat3 mut 3 utr, by GenePharma (1 mentions)
Dual luciferase reporter assay system, by Promega (1 mentions)
Lipo6000 transfection reagent, by Beyotime (1 mentions)
3 protocols using mir 21a 5p mimic
1
Modulating miR-21a-5p expression in MSCs
2
Validating miR-21a-5p Regulation of STAT3
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The miR-21a-5p target genes in the BV-2 cells were determined using the target prediction databases TARGETSCAN-VERT (http://www.targetscan.org ) and MIRDB (http://mirdb.org/miRDB/ ). The results showed that STAT3 is one of the target genes of miR-21a-5p. The plasmids pmirGLO-STAT3 WT-3′-UTR and pmirGLO-STAT3 MUT-3′-UTR were purchased from Gene Pharma (Suzhou, Jiangsu Province, China). BV-2 cells were transfected with the pmirGLO-WT/Mut-STAT3, miR-21a-5p mimics, and miR-21a-5p NC (Gene Pharma), using Lipofectamine 2000 according to the manufacturer’s instructions (Thermo Fisher Scientific). A Dual-Luciferase Reporter Assay System (Promega Corporation, Madison, WI, USA) was used to detect firefly and renilla luciferase activity.
3
Transfecting miRNA into Podocyte Cells
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miRNA mimics NC or miR-21a-5-p mimics (GenePharma Shanghai) were transfected into podocyte MPC5 cells using Lipo6000 transfection reagent (C0526FT, Beyotime, Shanghai, China) according to the specific manufacturer’s instructions. The transfection lasted for 48 h, and the podocyte was collected for experiments.
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