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I3 plate reader

Manufactured by Molecular Devices
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The I3 plate reader is a laboratory instrument designed for high-throughput analysis and detection of samples in microplates. It provides accurate and reliable measurement of fluorescence, luminescence, and absorbance.

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Lab products found in correlation

Softmax pro 6, by Molecular Devices (2 mentions) Ramos blue cells, by InvivoGen (1 mentions) Quanti blue, by InvivoGen (1 mentions)

Spelling variants of this product

SpectraMax i3 plate reader (89 citations) SpectraMax i3 multi-mode plate reader (7 citations) I3 multimode plate reader (2 citations) SpectraMax i3 fluorescent plate reader (2 citations)

4 protocols using i3 plate reader

1

Quantifying PLGA Biodistribution in Mice

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Two weeks before PLGA or saline injection and imaging of mice, animals were switched to a low-fluorescence, alfalfa-free diet (Research Diets, TD.97184 (purified)), provided ad libitum. Mice (n = 1/group) were fasted overnight (12–14 h) and then injected through a penile vein with 30 mg/kg of PLGA-Eu. Four hours later, mice were euthanized and organs depicted in Fig. 1 were extracted. The organs were positioned on a low-fluorescence mat that was placed on a 96-well plate insert of Molecular Devices i3 plate reader with a TRF module (WB cartridge) and the plate was imaged using excitation of 340 nm and emission 615 nm. Large Stokes shift of europium coupled with TRF tracking allowed for minimal background auto-fluorescence and high signal-to-noise ratio imaging of PLGA disposal in the liver and throughout the digestive system. Images were analyzed using Molecular Devices SoftMax Pro 6.2 software.
Chaplin A., Gao H., Asase C., Rengasamy P., Park B., Skander D., Bebek G., Rajagopalan S, & Maiseyeu A. (2020). Systemically-delivered biodegradable PLGA alters gut microbiota and induces transcriptomic reprogramming in the liver in an obesity mouse model. Scientific Reports, 10, 13786.
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2

Ramos-blue Cell Cytotoxicity Assay

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Each payload (as a stock solution in DMA) was diluted in a 5× serial dilution in 10% DMSO in PBS. Ramos-blue cells (InvivoGen, cat# rmssp) were cultured using high-glucose IMDM supplied with 10% FBS according to the manufacturer’s guidelines. The medium was supplemented with 50 μg/mL penicillin, 50 μg/mL streptomycin, 100 μg/mL normocin, and 500 μg/mL zeomycin to prevent bacterial contamination. Cells were seeded (in triplicate or quadruplicate) at a density of 0.2 × 106 cells/mL per well. 135 μL of the cell suspension was added to each well in a 96-well plate along with 15 μL of the corresponding payload. Cells were incubated at 37 °C under 5% CO2 for 24 or 72 h. The cells were centrifuged at 1990 rpm for 10 min, and 40 μL of the cell supernatant was added to 160 μL of the SEAP substrate (Quanti-Blue, InVivogen cat# repqbs). After incubation at 37 °C for 24 h, the absorbance at 630 nm was determined using a Molecular Devices i3× plate reader.
Fang S., Brems B.M., Olawode E.O., Miller J.T., Brooks T.A, & Tumey L.N. (2022). Design and Characterization of Immune-Stimulating Imidazo[4,5-c]quinoline Antibody-Drug Conjugates. Molecular pharmaceutics, 19(9), 3228-3241.
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3

Measuring Protein Diffusion Interaction

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Samples dialyzed against 10 mM histidine·HCl (pH 6.0) were diluted to 1.0 to 4.5 mg/ml in 0.5 mg/ml increments and loaded in triplicate into an AcroPrep 384-well 0.45-μm filter plate (Pall Life Sciences) set into a Greiner Bio-One UV-STAR 384-well plate. The plate sandwich was centrifuged briefly, and the apparent diffusion coefficient (Dapp) was measured in each well using a Wyatt DynaPro Plate Reader II dynamic light scattering instrument. Data were fit with the cumulants model, and the fidelity of the fit was verified by filtering the data with an SOS of 0.5. The absorbance of each well at 280 nm was determined using a Molecular Devices i3 plate reader. The mean Dapp (in cm2/s) of each triplicate sample was plotted as a function of the mean concentration (in grams per milliliter) and linearly regressed to determine the diffusion interaction parameter (kD; in milliliters per gram).
Kingsbury J.S., Saini A., Auclair S.M., Fu L., Lantz M.M., Halloran K.T., Calero-Rubio C., Schwenger W., Airiau C.Y., Zhang J, & Gokarn Y.R. (2020). A single molecular descriptor to predict solution behavior of therapeutic antibodies. Science Advances, 6(32), eabb0372.
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4

Nanoparticle Biodistribution in Mice

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Two weeks before the experiment, mice were switched to a low-fluorescence, alfalfa-free diet, provided ad libitum. Mice were fasted overnight (12-14 h) and then injected with 100 mg/kg of nanoparticles through a penile vein. Twenty four hours later, mice were euthanized and organs were extracted (depicted in Figure 3). The organs were positioned on a low-fluorescence mat that was placed on a 96-well plate insert of Molecular Devices i3 plate reader with a TRF module (WB cartridge) and the plate was imaged using excitation of 340 nm and emission 615 nm. Images were analyzed using Molecular Devices SoftMax Pro 6.2 software.
Mog B., Asase C., Chaplin A., Gao H., Rajagopalan S, & Maiseyeu A. (2019). Nano-Antagonist Alleviates Inflammation and Allows for MRI of Atherosclerosis. Nanotheranostics, 3(4), 342-355.
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