Human neuroblastoma SK-N-SH cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) under humidified atmosphere supplemented with 5% CO2 at 37°C. SK-N-SH cells were transfected with ΔC-TBP/Q36 or ΔC-TBP/Q79 construct by Lipofectamine 2000 (Thermo Fisher Scientific) and selected by 20 µg/mL of blasticidin (Sigma-Aldrich Co., St Louis, MO, USA) and the stable cell lines established. Expression EGFP-conjugated protein was induced by doxycycline (20 µg/mL, Thermo Fisher Scientific) for 4 days.
Sk n sh cells
Manufactured by Thermo Fisher Scientific
Sourced in United States
SK-N-SH cells are a neuroblastoma cell line derived from a human neuroblastoma tumor. They are used for in vitro studies related to neuroblastoma research and development.
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Lab products found in correlation
Sk n sh cells, by American Type Culture Collection (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin glutamine (psg), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions)
C11095500bt, by Thermo Fisher Scientific (2 mentions)
C11995500bt, by Thermo Fisher Scientific (2 mentions)
Hek293t cell, by American Type Culture Collection (2 mentions)
U251 cells, by American Type Culture Collection (2 mentions)
Ev71 strain vr 784, by National Collection of Authenticated Cell Cultures (2 mentions)
Doxycycline, by Thermo Fisher Scientific (1 mentions)
Blasticidin, by Merck Group (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Minimal essential medium nonessential amino acids, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
5 protocols using sk n sh cells
1
Inducible Expression of Mutant TBP in SK-N-SH Cells
2
Culturing NB9 and SK-N-SH Cell Lines
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NB9 cells were acquired from the RIKEN Cell Bank (Tsukuba, Japan) and were cultured in RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) with 15% fetal bovine serum (FBS) (Sigma Aldrich, St. Louis, MO) and 1% penicillin-streptomycin-glutamine (Thermo Fisher Scientific) at 37 °C in a 5% CO 2 incubator. Cells were subcultured to a fresh medium when the growth reached 90% confluence. SK-N-SH cells were acquired from the American Type Culture Collection (ATCC Manassas, VA, USA). SK-N-SH cells were cultured as monolayers in Dulbecco's modified Eagle medium (Thermo Fisher Scientific) with 10% FBS, 1% Minimal Essential Medium non-essential amino acids (Thermo Fisher Scientific), 1% sodium pyruvate (Thermo Fisher Scientific), and 1% penicillin-streptomycin-glutamine, at 37 °C in a 5% CO 2 incubator. Cells were subcultured to a fresh medium when the growth reached 90% confluence.
3
Culturing Human Neuroblastoma Cell Lines
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SK-N-SH cells (human neuroblastoma cell line) and SK-N-AS (p53-null cell line) were purchased from the Chinese Academy of Sciences (Beijing, China). As described by Huang et al. [24 (link)], SK-N-SH cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, USA) at 37°C in a 5% CO2 humidified atmosphere, with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin. When cells were70-80% confluent, Opti-MEM was used with 2 h prior to transfection.
4
Cultivation and Characterization of Cell Lines and Viruses
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HeLa, human rhabdomyosarcoma RD cells, human neuroblastoma SK-N-SH cells, human glioblastoma U251 cells, and HEK293T cells were obtained from the American Type Culture Collection (ATCC). SK-N-SH cells were cultured in MEM (C11095500BT; Gibco) containing 10% fetal bovine serum (FBS; Sunrise) and 100 U/ml of penicillin and streptomycin (10378016; Gibco). The other cells were cultured in DMEM (C11995500BT; Gibco) containing 10% FBS and 100 U/ml of penicillin and streptomycin. All cells were maintained in a humidified incubator with 5% CO2 at 37°C.
EV71 strain VR-784 was purchased from China Center for Type Culture Collection (CCTCC). EV71 strain SHAPHC695F/SH/CHN/10 (EV71-695F; GenBank:JQ736684.2 ) was isolated from fecal sample of a 1.8-yr-old patient in Shanghai public health clinical centre (SHPHC) in 2010 and provided by Dr. Zhigang Yi (Fudan University, Shanghai, China; Zhang et al., 2013 (link)). Coxsackievirus A16 (CVA16-G10; GeneBank: U05876.1 ) was provided by Dr. Dan Xu (Fudan University, Shanghai, China). VSV was from Genhong Cheng Laboratory (University of California, Los Angeles, CA, USA). HSV virus was kindly provided by Dr. Chunfu Zheng (Soochow University, Suzhou, China). Viral titers were determined using the TCID50 assay according to standard procedures.
EV71 strain VR-784 was purchased from China Center for Type Culture Collection (CCTCC). EV71 strain SHAPHC695F/SH/CHN/10 (EV71-695F; GenBank:
5
Cultivation and Characterization of Cell Lines and Viruses
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HeLa, human rhabdomyosarcoma RD cells, human neuroblastoma SK-N-SH cells, human glioblastoma U251 cells, and HEK293T cells were obtained from the American Type Culture Collection (ATCC). SK-N-SH cells were cultured in MEM (C11095500BT; Gibco) containing 10% fetal bovine serum (FBS; Sunrise) and 100 U/ml of penicillin and streptomycin (10378016; Gibco). The other cells were cultured in DMEM (C11995500BT; Gibco) containing 10% FBS and 100 U/ml of penicillin and streptomycin. All cells were maintained in a humidified incubator with 5% CO2 at 37°C.
EV71 strain VR-784 was purchased from China Center for Type Culture Collection (CCTCC). EV71 strain SHAPHC695F/SH/CHN/10 (EV71-695F; GenBank:JQ736684.2 ) was isolated from fecal sample of a 1.8-yr-old patient in Shanghai public health clinical centre (SHPHC) in 2010 and provided by Dr. Zhigang Yi (Fudan University, Shanghai, China; Zhang et al., 2013 (link)). Coxsackievirus A16 (CVA16-G10; GeneBank: U05876.1 ) was provided by Dr. Dan Xu (Fudan University, Shanghai, China). VSV was from Genhong Cheng Laboratory (University of California, Los Angeles, CA, USA). HSV virus was kindly provided by Dr. Chunfu Zheng (Soochow University, Suzhou, China). Viral titers were determined using the TCID50 assay according to standard procedures.
EV71 strain VR-784 was purchased from China Center for Type Culture Collection (CCTCC). EV71 strain SHAPHC695F/SH/CHN/10 (EV71-695F; GenBank:
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