All reagents used in the experiments were of analytical grade. Deionized water (resistivity ≥18 MΩ cm at 25°C) was obtained from a Milli-Q Advantage-0.10 purification system (Millipore). Human ACE2 Fc Chimera was obtained from GenScript. SP was kindly donated by Dr. Scott Hensley from the University of Pennsylvania. Graphene oxide conjugated with G-PEG amine functionalized, PEI, K3[Fe(CN)6], K4[Fe(CN)6], BSA, Nafion, EDAC, and NHS with a degree of purity ≥98% and phosphate buffer saline solution, pH = 7.4, were purchased from Sigma-Aldrich. Carbon and Ag/AgCl conductive inks of medical grade and dielectric ink were acquired from Creative Materials.
Milli q advantage 0.10 purification system
Manufactured by Merck Group
The Milli-Q Advantage-0.10 purification system is a laboratory water purification device designed to produce ultrapure water. It utilizes a combination of filtration and deionization technologies to remove impurities and contaminants from the input water, resulting in high-purity water with a resistivity of up to 18.2 megohm-cm.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffer saline solution, by Merck Group (3 mentions)
Polyethylenimine (pei), by Merck Group (2 mentions)
Nafion, by Merck Group (2 mentions)
Human ace2 fc chimera, by GenScript (2 mentions)
Graphene oxide conjugated with g peg amine functionalized, by Merck Group (2 mentions)
Glutaraldehyde, by Merck Group (2 mentions)
Cysteamine hydrochloride cys, by Merck Group (2 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Gold 3 chloride trihydrate, by Merck Group (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (1 mentions)
N hydroxysuccinimide, by Merck Group (1 mentions)
Sodium borohydride nabh4, by Merck Group (1 mentions)
Ace2 fc chimera, by GenScript (1 mentions)
Gold 3 chloride trihydrate haucl4 3h2o, by Merck Group (1 mentions)
4 protocols using milli q advantage 0.10 purification system
1
Electrochemical Sensor for COVID-19 Spike Protein
2
Electrochemical Detection of SARS-CoV-2 Spike Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All reagents used in this work were of analytical grade. The deionized water (resistivity ≥18 MΩ cm at 25 °C) was obtained from a Milli-Q Advantage-0.10 purification system (Millipore). ACE2 Fc Chimera, Human was obtained from GenScript. Spike protein was kindly donated by Scott Hensley, University of Pennsylvania, Philadelphia. EDC and NHS with a degree of purity ≥98%, gold(III) chloride trihydrate (HAuCl40.3H2O) (99.99%), sodium borohydride (NaBH4) with ≥98% purity, cysteamine hydrochloride (cys) with 98% purity, phosphate buffer saline solution, pH = 7.4, and glutaraldehyde (25%, vol/vol) were purchased from Sigma-Aldrich. Graphite pencils 0.7-mm and 0.9-mm diameter under the trade name of Pentel were purchased in a local store in Philadelphia. Ag/AgCl conductive ink was acquired from Creative Materials.
Electrochemical measurements were carried out using a MULTI AUTOLAB M101 potentiostat with six channels, controlled by the NOVA 2.1 software. Cryogenic vials were used as the electrochemical cell (2.0-mL volume), each containing three electrodes. Graphite pencils (0.9 mm) were used as reference and counterelectrodes, and graphite pencils (0.7 mm) were used as the WE. The Ag/AgCl ink was painted over one of the graphites to create the reference electrode.
Electrochemical measurements were carried out using a MULTI AUTOLAB M101 potentiostat with six channels, controlled by the NOVA 2.1 software. Cryogenic vials were used as the electrochemical cell (2.0-mL volume), each containing three electrodes. Graphite pencils (0.9 mm) were used as reference and counterelectrodes, and graphite pencils (0.7 mm) were used as the WE. The Ag/AgCl ink was painted over one of the graphites to create the reference electrode.
3
SARS-CoV-2 Spike Protein Functionalization
4
Electrochemical Sensor for COVID-19 Spike Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All reagents used in the experiments were of analytical grade. Deionized water (resistivity ≥18 MΩ cm at 25°C) was obtained from a Milli-Q Advantage-0.10 purification system (Millipore). Human ACE2 Fc Chimera was obtained from GenScript. SP was kindly donated by Dr. Scott Hensley from the University of Pennsylvania. Graphene oxide conjugated with G-PEG amine functionalized, PEI, K3[Fe(CN)6], K4[Fe(CN)6], BSA, Nafion, EDAC, and NHS with a degree of purity ≥98% and phosphate buffer saline solution, pH = 7.4, were purchased from Sigma-Aldrich. Carbon and Ag/AgCl conductive inks of medical grade and dielectric ink were acquired from Creative Materials.
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