Proteospin

Example 20

Two plasma samples from a single preterm infant, one from the umbilical cord blood, and one from a peripheral blood sample taken on day of life 28, underwent abundant plasma protein removal (PROTEOSPIN, NORGEN) prior to 2D-electrophoresis using separation first by isoelectric focusing (pH range 3-8) and then by size (TGX PRECAST GEL, BIORAD). The resulting gels were compared for differential protein content. Six differentially expressed protein clusters were analyzed. Following trypsin digestion and tandem mass spectrometry using an LTQ-FT ion-trap/FTMS hybrid mass spectrometer (THERMOELECTRON), candidate proteins/peptides were identified as potential NET-inhibitory substances.

Example 20

Two plasma samples from a single preterm infant, one from the umbilical cord blood, and one from a peripheral blood sample taken on day of life 28, underwent abundant plasma protein removal (PROTEOSPIN, NORGEN) prior to 2D-electrophoresis using separation first by isoelectric focusing (pH range 3-8) and then by size (TGX PRECAST GEL, BIORAD). The resulting gels were compared for differential protein content. Six differentially expressed protein clusters were analyzed. Following trypsin digestion and tandem mass spectrometry using an LTQ-FT ion-trap/FTMS hybrid mass spectrometer (THERMOELECTRON), candidate proteins/peptides were identified as potential NET-inhibitory substances.

Example 20

Two plasma samples from a single preterm infant, one from the umbilical cord blood, and one from a peripheral blood sample taken on day of life 28, underwent abundant plasma protein removal (PROTEOSPIN, NORGEN) prior to 2D-electrophoresis using separation first by isoelectric focusing (pH range 3-8) and then by size (TGX PRECAST GEL, BIORAD). The resulting gels were compared for differential protein content. Six differentially expressed protein clusters were analyzed. Following trypsin digestion and tandem mass spectrometry using an LTQ-FT ion-trap/FTMS hybrid mass spectrometer (THERMOELECTRON), candidate proteins/peptides were identified as potential NET-inhibitory substances.