Rabbit anti ndrg1

Standard Western blots were undertaken using RIPA (radioimmunoprecipitation assay) lysates as previously described [16 (link)]. A total of 10–20 ug of lysate was resolved via SDS-PAGE. Primary antibodies were as follows: anti-vinculin (1:5000) (Cat#4650, Cell Signalling Technology, Danvers, MA, USA), mouse anti-β-actin (1:5000) (Cat#sc-69879, Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-TGM2 (1:1000) (Cat#ab2386, Abcam, Cambridge, UK), rabbit anti-FTL (Cat#ab69090, Abcam, Cambridge, UK), rabbit anti-ANXA6 (Cat#31026, Abcam, Cambridge, UK), mouse anti-MVP (Cat#ab97311, Abcam, Cambridge, UK), rabbit anti-NDRG1 (Cat#5196S, Cell Signalling Technology, Danvers, MA, USA), and rabbit anti-SOD2 (Cat#13194S, Cell Signalling Technology, Danvers, MA, USA). HRP-conjugated goat anti-mouse (Cat#1706516, Bio-Rad, Hercules, CA, USA) and goat anti-rabbit (Cat#1706515, Bio-Rad, Hercules, CA, USA) secondary antibodies were used at 1:5000 and 1:3000, respectively.