Baf645

Manufactured by R&D Systems

BAF645 is a laboratory instrument designed for the analysis and quantification of biological samples. It utilizes advanced technologies to provide accurate and reliable data. The core function of BAF645 is to facilitate the measurement and evaluation of specific analytes in a range of research and diagnostic applications.

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Lab products found in correlation

Irdye 800cw goat anti rat, by LI COR (2 mentions) Odyssey blocking buffer, by LI COR (2 mentions) Odyssey system, by LI COR (2 mentions) Protease inhibitor cocktail, by Merck Group (2 mentions) Nupage 4 12 bis tris gradient gel, by Thermo Fisher Scientific (2 mentions) Irdye 800cw goat anti rabbit, by LI COR (2 mentions) Bis tris gel, by Thermo Fisher Scientific (2 mentions) Hif2a nb100 132b, by Novus Biologicals (2 mentions) Hif1a, by Cayman Chemical (1 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (1 mentions) Alexa fluor series, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Mab6838, by R&D Systems (1 mentions)

3 protocols using baf645

Western Blot Analysis of Ear Tissue

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Example 4

Western Analysis.

Ear tissue samples (3 ear hole donuts/ear from 3 separate mice) were homogenized in radio-immunoprecipitation assay buffer (50 mM Tris-HCl pH 7.6, containing 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 1 mM EDTA and 0.1% SDS) with 1 mM PMSF and a protease inhibitor cocktail (Sigma). Samples with equal amounts of protein (about 40 μg) were loaded into a NuPAGE 4-12% Bis-Tris gradient gel or 8% Bis-Tris gel (Life Technologies, Grand Island, N.Y.), electrophoresed and then electro-transferred onto a PVDF-FL membrane (Immobilon, Billerica, Mass.). The membrane was subsequently blocked with Odyssey blocking buffer (LI-COR, Lincoln, Nebr.), probed with primary antibodies (HIF1a (Ser. No. 10/006,421, Cayman Chemical, Ann Arbor, Mich.), HIF2a (NB100-132B, Novus, Littleton, Colo.), Wnt5a (BAF645, R&D System) or a-Tubulin (Sigma) overnight at 4° C., then further incubated with Alexa Fluor-labeled secondary antibodies (IRDye 800CW goat-anti rat or IRDye 800CW goat-anti rabbit (LI-COR, Lincoln, Nebr.) for 1 hr and scanned using the Odyssey system (LI-COR, Lincoln, Nebr.).

US11033541B2. Epimorphic regeneration and related hydrogel delivery systems (2021-06-15). Northwestern University [US], The Wistar Institute of Anatomy and Biology [US]. Inventors: Phillip B. Messersmith [US], Iossif A. Strehin [US], Ellen Heber-Katz [US].

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Western Blotting Analysis of Ear Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

Western Analysis.

Ear tissue samples (3 ear hole donuts/ear from 3 separate mice) were homogenized in radio-immunoprecipitation assay buffer (50 mM Tris-HCl pH 7.6, containing 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 1 mM EDTA and 0.1% SDS) with 1 mM PMSF and a protease inhibitor cocktail (Sigma). Samples with equal amounts of protein (about 40 μg) were loaded into a NuPAGE 4-12% Bis-Tris gradient gel or 8% Bis-Tris gel (Life Technologies, Grand Island, N.Y.), electrophoresed and then electro-transferred onto a PVDF-FL membrane (Immobilon, Billerica, Mass.). The membrane was subsequently blocked with Odyssey blocking buffer (LI-COR, Lincoln, Nebr.), probed with primary antibodies (HIF1a (10006421, Cayman Chemical, Ann Arbor, Mich.), HIF2a (NB100-132B, Novus, Littleton, Colo.), Wnt5a (BAF645, R&D System) or a-Tubulin (Sigma) overnight at 4° C., then further incubated with Alexa Fluor-labeled secondary antibodies (IRDye 800CW goat-anti rat or IRDye 800CW goat-anti rabbit (LI-COR, Lincoln, Nebr.) for 1 hr and scanned using the Odyssey system (LI-COR, Lincoln, Nebr.).

US10307415B2. Epimorphic regeneration and related hydrogel delivery systems (2019-06-04). Northwestern University [US], The Wistar Institute of Anatomy and Biology [US]. Inventors: Phillip B. Messersmith [US], Iossif A. Strehin [US], Ellen Heber-Katz [US].

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Immunofluorescence Staining of Cell Markers

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Samples were fixed in 4% paraformaldehyde for 20 minutes followed by 1 hour treatment with blocking buffer (0.2% each of triton-x, BSA, gelatin and casein and 0.02% sodium azide). Cells were incubated in primary antibody and incubated overnight at 4°C. Following day, cells were washed in PBS and incubated with appropriate secondary antibodies (Alexa fluor series, Invitrogen, 1:2000) at room temperature for 1 hour. Cells were washed in PBS, incubated with DAPI (Invitrogen, 1:10,000) and mounted in Prolong Gold anti-fade reagent (Invitrogen). Images were captured on a Leica TCS SPII scanning laser confocal system. Primary antibodies used were as follows: Ki67 (1:400, #9027, Cell Signaling), sFRP2 (1:100, MAB6838, R&D Systems), biotinylated Wnt5A (500 ng/mL, BAF645, R&D Systems).

Fane M., Ecker B.L., Kaur A., Marino G.E., Alicea G., Douglass S., Chhabra Y., Webster M.R., Marshall A., Colling R., Espinosa O., Coupe N., Maroo N., Campo L., Middleton M.R., Corrie P., Xu X., Karakousis G.C, & Weeraratna A.T. (2020). SFRP2 supersedes VEGF as an age-related driver of angiogenesis in melanoma, affecting response to anti-VEGF therapy in older patients.. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(21), 5709-5719.

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