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Trehalose 6 6 dibehenate

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Trehalose-6,6-dibehenate is a synthetic compound consisting of the disaccharide trehalose linked to two behenate (C22:0) fatty acid chains. It is a water-soluble, non-toxic molecule that can be used as a stabilizing agent for various biological molecules and formulations.

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Lab products found in correlation

Gm csf, by InvivoGen (1 mentions) Lipopolysaccharides (lps), by InvivoGen (1 mentions) Zymosan tlrl zyn, by InvivoGen (1 mentions) Hkca tlrl hkca, by InvivoGen (1 mentions) Stat3 in 1, by MedChemExpress (1 mentions) Sis17, by MedChemExpress (1 mentions) L nmma, by Beyotime (1 mentions) Jsh 23, by MedChemExpress (1 mentions) Bafilomycin, by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) Halofuginone, by Santa Cruz Biotechnology (1 mentions) Prostaglandin e2 pge2, by Merck Group (1 mentions) Tlrl 1826, by InvivoGen (1 mentions) Tlrl picw, by Merck Group (1 mentions) Forskolin, by Cayman Chemical (1 mentions) Fla bs, by InvivoGen (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Tunicamycin, by Merck Group (1 mentions) Tlrl r848, by InvivoGen (1 mentions) Tert butylhydroquinone tbhq, by Merck Group (1 mentions)

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Trehalose‐6,6‐dibehenate (TDB, (4 citations)

3 protocols using trehalose 6 6 dibehenate

1

Pleural Immune Cell Activation Assay

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Pleural immune cells were cultured in RPMI-1640 GlutaMax supplemented with 10% FCS, 25mM of Hepes, 1 mM sodium pyruvate, 100U/ml of Penicillin-Streptomycin and 20µg/ml of Gentamicin at 37°C in the presence of 5% CO2. Pleural immune cells were stimulated in 12-well plates (106 cells/ml) during 24h by LPS (2µg/ml), Nec-1s (50µM), GM-CSF (20ng/ml) or Trehalose-6,6-dibehenate (TDB, a Mincle agonist) (10µg/ml) (Invivogen). Then supernatants were collected for cytokine measurement. For analyzing the level of phosphorylation of Syk by flow cytometry, PS cells were stimulated during 30min by TDB (10µg/ml).
Bénard A., Podolska M.J., Czubayko F., Kutschick I., Klösch B., Jacobsen A., Naschberger E., Brunner M., Krautz C., Trufa D.I., Sirbu H., Lang R., Grützmann R, & Weber G.F. (2022). Pleural Resident Macrophages and Pleural IRA B Cells Promote Efficient Immunity Against Pneumonia by Inducing Early Pleural Space Inflammation. Frontiers in Immunology, 13, 821480.
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2

Immune Response Modulation Protocols

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Heat-killed C. albicans (HKCA; tlrl-hkca), zymosan (tlrl-zyn) and Trehalose-6,6-dibehenate (TDB; tlrl-tdb) were purchased from Invivogen. Mannan (M3640) were purchased from Sigma-Aldrich. The HDAC11 inhibitors FT895 (HY-112285) and SIS17 (HY-128918), SYK inhibitor R406 (HY-12067), NF-κB inhibitor JSH-23 (HY-13982) and STAT3 inhibitor STAT3-IN-1 (HY-100753) were purchased from MedChemExpress. iNOS inhibitors S-methylisothiourea hemisulfate salt (SMT; S0008) and L-NMMA (S0011) were purchased from Beyotime Biotechnology. The SYK inhibitor R406 (S2194) was purchased from Selleck. The antibodies used in this study shown in Supplementary Table 1.
Wu H., Yin X., Zhao X., Wu Z., Xiao Y., Di Q., Sun P., Tang H., Quan J, & Chen W. (2022). HDAC11 negatively regulates antifungal immunity by inhibiting Nos2 expression via binding with transcriptional repressor STAT3. Redox Biology, 56, 102461.
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3

Innate Immune Stimuli Combination Protocol

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300 ng/mL Pam3Cys-SKKKK (EMC-microcollections L2000), 20 μg/mL LMW polyinosinicpolycytidylic acid (InvivoGen tlrl-picw), 100 ng/mL lipopolysaccharides E. coli 055:B5 (Sigma L6529), 5 μg/mL FLA (B. subtilis) ~32 kDa Gram-positive flagellin (InvivoGen FLA-BS), 10 μg/mL R848 imidazoquinoline compound (InvivoGen tlrl-r848), 5 μM CpG (ODN 1826) unmethylated CpG dinucleotides (InvivoGen tlrl-1826), 10 μg/mL muramyl dipeptide (InvivoGen tlrl-mdp), 10 μg/mL synthetic analog of trehalose-6,6- dimycolate (TDB, Trehalose-6,6-dibehenate) (InvivoGen tlrl-tdb), MOI ~10 Sendai virus (Sendai strain: Cantell) (ATCC VR-907), 5 μg/mL zymosan (S. cerevisiae) (Sigma Z4250), 20 μg/mL HT-DNA (herring testis DNA) (Trevigen). DMSO (Sigma), 10 μM forskolin (Cayman Chemical), 25 μM tert-Butylhydroquinone (tBHQ) (Sigma), 5 μM prostaglandin E2 (pGE2) (Sigma), 1 μM torin (Axon Medchem), 10 μM halofuginone (Santa Cruz Biotechnology), 100 nM bafilomycin (Sigma), 20 μM ZVAD pan-caspase inhibitor (Calbiochem), caspase I inhibitor IV (Calbiochem), 5 μg/mL brefeldin A, 5 μg/mL tunicamycin (Sigma).
O'Connell D.J., Kolde R., Sooknah M., Graham D.B., Sundberg T.B., Latorre I., Mikkelsen T.S, & Xavier R.J. (2016). Simultaneous Pathway Activity Inference and Gene Expression Analysis Using RNA Sequencing. Cell systems, 2(5), 323-334.
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