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Human ifn γ elispot kit

Manufactured by Abcam
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Sourced in United States

The Human IFN-γ ELISPOT Kit is a laboratory tool used to detect and quantify the production of interferon-gamma (IFN-γ) by human cells. It provides a sensitive and reliable method for measuring the frequency of IFN-γ-secreting cells in a sample.

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Lab products found in correlation

Immunospot plate reader, by Cellular Technology (1 mentions)

2 protocols using human ifn γ elispot kit

1

IFN-γ ELISPOT Assay for T Cell-Tumor Interaction

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Human IFN-γ ELISPOT Kit (with precoated plates, Abcam, USA) was performed as the protocol’s procedure. Briefly, 2 × 104 T cells, rested in cytokines-free media overnight, and 1 × 104 PBS-washed autologous tumor cells were incubated together for approximately 20 h in the absence of exogenous cytokines at 37 °C with 5% CO2. The number of colored spots was determined by ImmunoSpot plate reader and associated software (Celluar Technologies, USA).
Tan Q., Zhang C., Yang W., Liu Y., Heyilimu P., Feng D., Xing L., Ke Y, & Lu Z. (2019). Isolation of T cell receptor specifically reactive with autologous tumour cells from tumour-infiltrating lymphocytes and construction of T cell receptor engineered T cells for esophageal squamous cell carcinoma. Journal for Immunotherapy of Cancer, 7, 232.
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2

IFN-γ ELISPOT Assay for LMP2A Peptide

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Human IFN-γ ELISPOT Kit (Abcam, USA) was performed as the manufacturer’s protocols. Briefly, 2×104 T cells, rested in cytokine-free media overnight, were incubated with 1×104 PBS-washed autologous DCs pulsed with LMP2A peptide mix at a concentration of 10 µg/mL for approximately 20 hours in the absence of exogenous cytokines. The number of colored spots was calculated by ImmunoSpot plate reader and associated software (Cellular Technologies, USA).
Zhang C., Tan Q., Li S., Shen L., Zhang J., Liu Y., Yang W, & Lu Z. (2021). Induction of EBV latent membrane protein-2A (LMP2A)-specific T cells and construction of individualized TCR-engineered T cells for EBV-associated malignancies. Journal for Immunotherapy of Cancer, 9(7), e002516.
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